23个小麦抗叶锈病近等基因系SRAP多态性

doi:10.3864/j.issn.0578-1752.2008.05.010

中国农业科学 ›› 2008, Vol. 41 ›› Issue (5): 1333-1340 .doi: 10.3864/j.issn.0578-1752.2008.05.010

23个小麦抗叶锈病近等基因系SRAP多态性

刘雅辉,闫红飞,杨文香,李星,李亚宁,孟庆芳,张立荣,刘大群,张汀

河北农业大学植物病理系/河北省农作物病虫害生物防治工程技术研究中心

收稿日期:2007-03-29 修回日期:1900-01-01 出版日期:2008-05-10 发布日期:2008-05-10
通讯作者: 刘大群

Molecular Diversity of 23 Wheat Leaf Rust Resistance Near-isogenic Lines Determined by Sequence-Related Amplified Polymorphism

Ya-Hui LIU Hong-Fei RAN Wen-Xiang YANG Xing LI Ya-Ning LI Qing-Fang MENG Li-Rong ZHANG Ting ZHANG Da-Qun LIU

河北农业大学植物病理系/河北省农作物病虫害生物防治工程技术研究中心

Received:2007-03-29 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10

摘要/Abstract

摘要： 【目的】分析以Thatcher为遗传背景的小麦抗叶锈病近等基因系的遗传多样性，探讨SRAP技术在小麦抗叶锈病基因标记及基因克隆研究中应用的可行性。【方法】采用SRAP（sequence-related amplified polymorphism）技术对23个以Thatcher为遗传背景的小麦抗叶锈病近等基因系和感病对照Thatcher进行分析。【结果】从128对引物中筛选得到41对具有多态性引物，每个引物组合产生6～41个多态性条带，共产生537个多态性条带，多态性条带率达49.5%，平均每个引物组合产生13.1个多态性条带；每个引物组合产生1～13个特异性条带，共产生115个特异性条带，特异性条带率为10.6%，平均每个引物组合产生2.8个特异性条带。聚类分析将23个小麦抗叶锈病近等基因系和Thatcher在相似系数0.72处分为A、B两大类，其中96%的个体归入B类。B类又分为Ⅰ、Ⅱ两个亚类，95%的个体聚在第Ⅱ亚类。第Ⅱ亚类在相似系数为0.785附近分为4小类。【结论】23个小麦抗叶锈病近等基因系间存在一定的差异。SRAP技术是一种简单有效的分子标记系统，可在小麦叶锈病抗病基因的研究中应用。

关键词: 小麦抗叶锈病基因, 近等基因系, SRAP

Abstract: 【Objective】The objective of the research were analysis the Genetic diversity of Wheat Leaf Rust Resistance Near-isogenic Lines in Thatcher Background, and investigating the feasibility of SRAP marker applied on study of Wheat Leaf rust Resistance Genes marker and clone. 【Method】Total 23 wheat leaf rust resistance near-isogenic lines and Thatcher were carried out using SRAP(sequence-related amplified polymorphism)markers. 【Result】41of 128 SRAP primer combinations identified a total of 537 reproducible fragments. Among them, 49.5% were polymorphic, and 115 of them were specific bands (10.6%). Between 6 and 41 fragments were amplified per primer combination, with an average of 13.1 bands. The number of polymorphic fragments for each primer combination varied from 1 to 13, with an average of 2.8. A cluster analysis was performed using NTSYSpc Version 2.10e. Cluster analysis revealed 23 wheat leaf resistance genes and Thatcher into two clusters of A and B with the similarity coefficient of 0.72 . 96% of them were clustered into cluster B, which was divided into two groups,ⅠandⅡ. 95% of the near isogenic lines in cluster B fell into group Ⅱ. Group Ⅱ included four small groups with 0.785 similarity coefficient. 【Result】It was concluded that the 23 wheat leaf rust resistance near isogenic lines have more differences in molecular level. All these results showed SRAP marker is simple, economic and effective,it could be widely used in study of Wheat Leaf Rust resistance genes.

Key words: Wheat Leaf rust Resistance Genes, NILs, SRAP

刘雅辉,闫红飞,杨文香,李星,李亚宁,孟庆芳,张立荣,刘大群,张汀. 23个小麦抗叶锈病近等基因系SRAP多态性[J]. 中国农业科学, 2008, 41(5): 1333-1340 .

Ya-Hui LIU Hong-Fei RAN Wen-Xiang YANG Xing LI Ya-Ning LI Qing-Fang MENG Li-Rong ZHANG Ting ZHANG Da-Qun LIU. Molecular Diversity of 23 Wheat Leaf Rust Resistance Near-isogenic Lines Determined by Sequence-Related Amplified Polymorphism[J]. Scientia Agricultura Sinica, 2008, 41(5): 1333-1340 .

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23个小麦抗叶锈病近等基因系SRAP多态性

Molecular Diversity of 23 Wheat Leaf Rust Resistance Near-isogenic Lines Determined by Sequence-Related Amplified Polymorphism

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