应用SRAP分子标记构建红麻种质资源分子身份证

doi:10.3864/j.issn.0578-1752.2013.10.002

摘要/Abstract

摘要： 【目的】以来源于不同国家和地区的127份红麻栽培种、野生种和近缘种为材料，利用SRAP标记构建红麻种质资源分子身份证。【方法】利用SRAP标记对127份红麻种质进行遗传分析，计算其遗传相似系数。利用UPGMA法作聚类图，构建分子身份证。【结果】40对引物组合在127份红麻种质材料中共扩增出383条DNA片段，其中，375条为多态性片段，总的多态性条带比率（PPB）为97.9%。UPGMA聚类分析结果表明，相似系数为0.70时，127份红麻种质资源被划分为4个类群。用SRAP特征谱带和多种引物组合2种方法可有效区分所有材料，并构建出127份红麻种质资源特异性分子身份证，置信概率达到99.99%。【结论】基于15对SRAP核心引物组合的36条谱带构建了一套127份红麻种质资源唯一性的分子身份证。

关键词: 红麻种质资源 , SRAP标记 , DNA指纹 , 分子身份证

Abstract: 【Objective】One hundred and twenty-seven accessions of kenaf gemplasm collected from different countries and areas, which contain cultivated, wild and related species, were selected and SRAP markers were used to establish molecular identity of kenaf germplasm.【Method】 Sequence- related amplified polymorphism(SRAP) marker was used for analyzing genetic relationships of the 127 different type accessions. Nei's similarity coefficients were generated and dendrogram was constructed using UMPGA by NTSYS-PC program.【Result】Three hundred and eighty-three bands were amplified by 40 SRAP primers, of which 375 were polymorphic and PPB was 97.9%. The cluster analysis based on UPGMA showed that the 127 accessions of kenaf germplasm were divided into four major groups at similarity coefficient of 0.70. All of the accessions could be effectively distinguished by specific bands and different primer combinations. The specific molecular identity for 127 accessions of kenaf germplasm were established with 99.99% probability of confidence.【Conclusion】A set of unique molecular identity was established for 127 kenaf germplasm based on 36 bands which were amplified by 15 core primer combinations.

Key words: kenaf germplasm , SRAP marker , DNA fingerprint , molecular identity

刘倩, 戴志刚, 陈基权, 温岚, 龚友才, 粟建光. 应用SRAP分子标记构建红麻种质资源分子身份证[J]. 中国农业科学, 2013, 46(10): 1974-1983.

LIU Qian, DAI Zhi-Gang, CHEN Ji-Quan, WEN Lan, GONG You-Cai, SU Jian-Guang. Establishment of Molecular Identity for Kenaf Germplasm Using SRAP Marker[J]. Scientia Agricultura Sinica, 2013, 46(10): 1974-1983.

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