Arbuscular mycorrhizal fungi combined with exogenous calcium improves the growth of peanut (Arachis hypogaea L.) seedlings under continuous cropping
CUI Li1, 2, GUO Feng1, 2, ZHANG Jia-lei1, 2, YANG Sha1, 2, MENG Jing-jing1, 2, GENG Yun1, 2, WANG Quan1, 2, 3, LI Xin-guo1, 2, WAN Shu-bo2, 4
1 Biotechnology Research Center, Shandong Academy of Agricultural Sciences/Key Laboratory of Crop Genetic Improvement and Ecological Physiology of Shandong Province, Jinan 250100, P.R.China 2 Scientific Observing and Experimental Station of Crop Cultivation in East China, Ministry of Agriculture, Jinan 250100, P.R.China 3 College of Life Sciences, Shandong Normal University, Jinan 250014, P.R.China 4 Shandong Academy of Agricultural Sciences, Jinan 250100, P.R.China
The growth and yield of peanut are negatively affected by continuous cropping. Arbuscular mycorrhizal fungi (AMF) and calcium ions (Ca2+) have been used to improve stress resistance in other plants, but little is known about their roles in peanut seedling growth under continuous cropping. This study investigated the possible roles of the AMF Glomus mosseae combined with exogenous Ca2+ in improving the physiological responses of peanut seedlings under continuous cropping. G. mosseae combined with exogenous Ca2+ can enhance plant biomass, Ca2+ level, and total chlorophyll content. Under exogenous Ca2+ application, the Fv/Fm in arbuscular mycorrhizal (AM) plant leaves was higher than that in the control plants when they were exposed to high irradiance levels. The peroxidase, superoxide dismutase, and catalase activities in AM plant leaves also reached their maximums, and accordingly, the malondialdehyde content was the lowest compared to other treatments. Additionally, root activity, and content of total phenolics and flavonoids were significantly increased in AM plant roots treated by Ca2+ compared to either G. mosseae inoculation or Ca2+ treatment alone. Transcription levels of AhCaM, AhCDPK, AhRAM1, and AhRAM2 were significantly improved in AM plant roots under exogenous Ca2+ treatment. This implied that exogenous Ca2+ might be involved in the regulation of G. mosseae colonization of peanut plants, and in turn, AM symbiosis might activate the Ca2+ signal transduction pathway. The combination of AMF and Ca2+ benefitted plant growth and development under continuous cropping, suggesting that it is a promising method to cope with the stress caused by continuous cropping.
Fund: This work was supported by the National Natural Science Foundation of China (31601261, 31601252, 31571581 and 31571605) and the China Postdoctoral Science Foundation (2016M592236).
Corresponding Authors: Correspondence LI Xin-guo, Tel: +86-531-66659047, E-mail: email@example.com; WAN Shu-bo, Tel: +86-531-66658217, E-mail: firstname.lastname@example.org
Cite this article:
CUI Li, GUO Feng, ZHANG Jia-lei, YANG Sha, MENG Jing-jing, GENG Yun, WANG Quan, LI Xinguo, WAN Shu-bo. 2019. Arbuscular mycorrhizal fungi combined with exogenous calcium improves the growth of peanut (Arachis hypogaea L.) seedlings under continuous cropping. Journal of Integrative Agriculture,