Up-regulation of a homeodomain-leucine zipper gene HD-1 contributes to trichome initiation and development in cotton
NIU Er-li, CAI Cai-ping, BAO Jiang-hao, WU Shuang, ZHAO Liang, GUO Wang-zhen
State Key Laboratory of Crop Genetics & Germplasm Enhancement, Ministry of Science and Technology/Hybrid Cotton R&D Engineering Research Center, Ministry of Education/College of Agriculture, Nanjing Agricultural University, Nanjing 210095, P.R.China
Plant trichomes originate from epidermal cells. In this work, we demonstrated that a homeodomain-leucine zipper (HD-Zip) gene, Gh_A06G1283 (GhHD-1A), was related to the leaf trichome trait in allotetraploid cotton and could be a candidate gene for the T1 locus. The ortholog of GhHD-1A in the hairless accession Gossypium barbadense cv. Hai7124 was interrupted by a long terminal repeat (LTR) retrotransposon, while GhHD-1A worked well in the hairy accession Gossypium hirsutum acc. T586. Sequence and phylogenetic analysis showed that GhHD-1A belonged to the HD-Zip IV gene family, which mainly regulated epidermis hair development in plants. Silencing of GhHD-1A and its homoeologs GhHD-1D in allotetraploid T586 and Hai7124 could significantly reduce the density of leaf hairs and affect the expression levels of other genes related to leaf trichome formation. Further analysis found that GhHD-1A mainly regulated trichome initiation on the upper epidermal hairs of leaves in cotton, while the up-regulated expression of GhHD-1A in different organs/tissues also altered epidermal trichome development. This study not only helps to unravel the important roles of GhHD-1A in regulating trichome initiation in cotton, but also provides a reference for exploring the different forms of trichome development in plants.
About author: NIU Er-li, E-mail: firstname.lastname@example.org;
Cite this article:
NIU Er-li, CAI Cai-ping, BAO Jiang-hao, WU Shuang, ZHAO Liang, GUO Wang-zhen. 2019. Up-regulation of a homeodomain-leucine zipper gene HD-1 contributes to trichome initiation and development in cotton. Journal of Integrative Agriculture,