? The characterization of acid and pepsin soluble collagen from ovine bones (Ujumuqin sheep)
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    2018, Vol. 17 Issue (03): 704-711     DOI: 10.1016/S2095-3119(17)61751-9
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The characterization of acid and pepsin soluble collagen from ovine bones (Ujumuqin sheep)
GAO Ling-ling, WANG Zhen-yu, LI Zheng, ZHANG Cai-xia, ZHANG De-quan  
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Processing, Ministry of Agriculture, Beijing 100193, P.R.China
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Abstract Ovine bones are the major by-products after slaughtered.  The present study was conducted to extract and characterize acid soluble collagens (ASC) and pepsin soluble collagens (PSC) from ovine bones (Ujumuqin sheep).  Ovine bones collagen were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) as type I collagen.  The results of Fourier transform infrared (FTIR) spectra analysis testified the existence of triple superhelical structure in both ASC and PSC, showing pepsin did not disrupt the triple helical structure of ovine bones collagen.  Glycine, accounting for one-third of total amino acids, was the major amino acid for ovine bones collagen.  Higher imino acid content was responsible for higher thermal denaturation temperature of ovine bones collagen compared to fish collagens.  The isoelectric point of ASC was lower than PSC due to the higher content of acidic amino acids.  Therefore, this study provides the potential reference for collagen extraction and application of ovine bones by-procduct.
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Key words�� ovine bones     collagen     characterization     amino acid composition     denaturation temperature     
Received: 2017-05-11; Published: 2017-06-07

This work has been funded by the emarked fund for China Agriculture Research System (CARS-39), the National Agricultural Science and Technology Innovation Program.

Corresponding Authors: Correspondence ZHANG De-quan, Tel/Fax: +86-10-62818740, E-mail: dequan_zhang0118@126.com   
About author: GAO Ling-ling, E-mail: gaolinglingwork@163.com;
Cite this article:   
GAO Ling-ling, WANG Zhen-yu, LI Zheng, ZHANG Cai-xia, ZHANG De-quan. The characterization of acid and pepsin soluble collagen from ovine bones (Ujumuqin sheep)[J]. Journal of Integrative Agriculture, 2018, 17(03): 704-711.
http://www.chinaagrisci.com/Jwk_zgnykxen/EN/ 10.1016/S2095-3119(17)61751-9      or     http://www.chinaagrisci.com/Jwk_zgnykxen/EN/Y2018/V17/I03/704
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