MAPKs and acetyl-CoA are associated with Curvularia lunata pathogenicity and toxin production in maize
NI Xuan, GAO Jin-xin, YU Chuan-jin, WANG Meng, Sun Jia-nan, LI Ya-qian, CHEN Jie
School of Agriculture and Biology, Shanghai Jiao Tong University/State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai 200240, P.R.China
Abstract Mitogen-activated protein kinase (MAPK) cascades play an important role in extracellular signal transduction and are involved in the pathogenicity of fungal pathogens to host plants. In Curvularia lunata, the roles of two MAPK genes, Clk1 and Clm1, have already been studied. Clk1 is involved in conidia formation and pathogenicity, and Clm1 is closely related to pathogen cell wall formation and pathogenicity to maize leaves. In this study, a third C. lunata MAPK gene, Clh1, which is homologous to hog1, was successfully cloned. We found that a Clh1 deletion mutant had lower intracellular glycerol accumulation than the wild-type stain and was unable to grow normally under osmotic stress conditions. Furthermore, the deletion mutants of three C. lunata MAPK genes (Clk1, Clm1 and Clh1) had lower levels of acetyl-CoA, which is an important intermediate product in the synthesis of melanin and furan toxin, and down-regulated expression of pathogenicity-associated genes. Furthermore, pathogenicity and the ability to produce toxin were restored after adding acetyl-CoA to the culture medium, suggesting that acetyl-CoA is closely involved in the pathogen MAPK signaling pathway.
This work was supported by the National Natural Science Foundation of China (31471734 and 31672072) and the earmarked fund for China Agriculture Research System (CARS-02). All authors have declared no conflict of interest.
NI Xuan, GAO Jin-xin, YU Chuan-jin, WANG Meng, Sun Jia-nan, LI Ya-qian, CHEN Jie. MAPKs and acetyl-CoA are associated with Curvularia lunata pathogenicity and toxin production in maize[J]. Journal of Integrative Agriculture,
2018, 17(01): 139-148.
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