Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization
LIU Xue1, 2, ZHU Chang-xiong1, Frank Delvigne2, GONG Ming-bo3
1 Institute of Agricultural Environment and Sustainable Development, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China 2 Université de Liège, Gembloux Agro-Bio Tech, Unité de Bio-Industries, Gembloux 5030, Belgium 3 Key Laboratory of Microbial Resources, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
Abstract A primary cDNA library of Aspergillus niger H1 was constructed using the switching mechanism at the 5´ end of the RNA transcript (SMART) technique. A total of 169 clones exhibited halos when grown on tricalcium phosphate medium, and the H-46 clone displayed a clear halo. The full-length cDNA of the clone H-46 clone was 1 407 bp in length with a complete open reading frame (ORF) of 816 bp, and it encoded a protein that contained 272 amino acids. Multiple alignment analysis revealed a high degree of homology between the ORFs of the H-46 clone and the Bax inhibitor family (BI-1-like) proteins of other fungi. Acetic acid was secreted by Escherichia coli DH5α that express the BI-1-like gene. The level attained was 492.52 mg L−1, which was associated with the release of 0.212 mg mL−1 of soluble phosphate at 28 h. These results showed that the heterologous expression of BI-1-like genes in Eschericha coli DH5α increased the secretion of acetic acid by altering the membrane permeability and enhancing the solubility of phosphate (P).
This work was supported by the National Natural Science Foundation of China (41440008).
Corresponding Authors: Correspondence GONG Ming-bo, Tel: +86-10-82108683, E-mail: firstname.lastname@example.org
About author: LIU Xue, Tel: +86-10-82108683, E-mail: email@example.com;
Cite this article:
LIU Xue, ZHU Chang-xiong, Frank Delvigne, GONG Ming-bo. Screening of genes of secreting acetic acid from Aspergillus niger H1 to improve phosphate solubilization[J]. Journal of Integrative Agriculture,
2017, 16(09): 2110-2114.
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