Effect of yeast Saccharomyces cerevisiae supplementation on serum antioxidant capacity, mucosal sIgA secretions and gut microbial populations in weaned piglets
ZHU Cui1, 2*, WANG Li1*, WEI Shao-yong1, CHEN Zhuang2, MA Xian-yong1, ZHENG Chun-tian1, JIANG Zong-yong1, 2
1 Key Laboratory of Animal Nutrition and Feed (South China), Ministry of Agriculture/Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China 2 Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China
Abstract This study was conducted to determine the effect of different forms of yeasts Saccharomyces cerevisiae supplementation on serum antioxidant capacity, mucosal secretory immunoglobulin A (sIgA) secretions and gut microbial populations in weaned piglets. A total of 96 piglets weaned at 14 d of age were randomly allotted to 4 dietary treatments: (1) basal diet without yeast (Control); (2) basal diet supplemented with 3.00 g kg–1 live yeast (LY); (3) basal diet supplemented with 2.66 g kg–1 heat-killed whole yeast (HKY); and (4) basal diet supplemented with 3.00 g kg–1 superfine yeast powders (SFY). Each treatment had 4 replicates (pens), with 6 piglets per replicate. The experiment lasted for 3 wk. At d 7 and 21 of the experiment, the samples of serum, mucosa and mesenteric lymph node (MLN) from jejunum, and digesta from the ileum and cecum were collected for determinations. Compared with the Control, dietary SFY supplementation increased serum superoxide dismutase (SOD) activity and lysozyme levels at d 7, and jejunum mucosal sIgA secretions at d 21 of the experiment (P<0.05). Dietary LY supplementation increased serum SOD activity and jejunum mucosal sIgA secretions, but decreased serum malondialdehyde (MDA) concentration at d 7 and 21 (P<0.05). Piglets fed diets supplemented with LY and SFY had lower pH values and decreased numbers of Escherichia coli in the ileum and cecum contents at d 21 compared with the Control (P<0.05). Moreover, the ratio of Lactobacilli to E. coli in the ileum and cecum contents was increased by dietary LY and SFY supplementations (P<0.05). Collectively, different forms of yeasts, especially LY and SFY, may modulate body antioxidant capacity and enhance the intestinal immunity by regulation of secretions of mucosal sIgA and reduction of pathogenic bacteria colonization, thus improving intestinal health of weaned piglets.
This study was financially supported by grants from the National Natural Science Foundation of China (31472112 and 31501967), the China Agriculture Research System (CARS-36), the Special Fund for Agro-scientific Research in the Public Interest, China (201403047), the Science and Technology Program of Guangdong Province, China (2013A061401020, 2013B020306004, 2016A020210041, 2016B070701013), the Hundred Outstanding Talents Training Program at Guangdong Province, China, the Science and Technology Program of Guangzhou, China (201607020035), and the Presidential Foundation of Guangdong Academy of Agricultural Sciences, China (201612).
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