A callus transformation system for gene functional studies in soybean
XU Kun1*, ZHANG Xiao-mei1*, FAN Cheng-ming1, CHEN Fu-lu1, ZHU Jin-long1, ZHANG Shi-long2, CHEN Qing-shan3, FU Yong-fu1
1 Key Lab of Soybean Biology (Beijing), Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China 2 Bijie Agricultural Science Research Institute of Guizhou, Bijie 551700, P.R.China 3 College of Agriculture, Northeast Agricultural University, Harbin 150030, P.R.China
Abstract Obtaining transgenic plants is a common method for analyzing gene function. Unfortunately, stable genetic transformation is difficult to achieve, especially for plants (e.g., soybean), which are recalcitrant to genetic transformation. Transient expression systems, such as Arabidopsis protoplast, Nicotiana leaves, and onion bulb leaves are widely used for gene functional studies. A simple method for obtaining transgenic soybean callus tissues was reported recently. We extend this system with simplified culture conditions to gene functional studies, including promoter analysis, expression and subcellular localization of the target protein, and protein-protein interaction. We also evaluate the plasticity of this system with soybean varieties, different vector constructs, and various Agrobacterium strains. The results indicated that the callus transformation system is efficient and adaptable for gene functional investigation in soybean genotype-, vector-, and Agrobacterium strain-independent modes. We demonstrated an easy set-up and practical homologous strategy for soybean gene functional studies.
This work was supported by the Transgenic Programs, China (2014ZX0800930B and 2016ZX08009-001) and the National Natural Science Found of China (31371703).
Corresponding Authors: Correspondence FU Yong-fu, Tel/Fax: +86-10-82105864, E-mail: email@example.com, firstname.lastname@example.org
About author: XU Kun, E-mail: email@example.com; ZHANG Xiao-mei, E-mail: firstname.lastname@example.org;
* These authors contributed equally to this study.
Cite this article:
XU Kun, ZHANG Xiao-mei, FAN Cheng-ming, CHEN Fu-lu, ZHU Jin-long, ZHANG Shi-long, CHEN Qing-shan, FU Yong-fu. A callus transformation system for gene functional studies in soybean[J]. Journal of Integrative Agriculture,
2017, 16(09): 1913-1922.
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