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Colonization by Klebsiella variicola FH-1 stimulates soybean growth and alleviates the stress of Sclerotinia sclerotiorum

ZHAI Qian-hang, PAN Ze-qun, ZHANG Cheng, YU Hui-lin, ZHANG Meng, GU Xue-hu, ZHANG Xiang-hui, PAN Hong-yu, ZHANG Hao

2023, 22 (9): 2729-2745. DOI: 10.1016/j.jia.2023.01.007

Abstract （240） PDF in ScienceDirect

Sclerotinia stem rot, caused by Sclerotinia sclerotiorum, is a destructive soil-borne disease leading to huge yield loss. We previously reported that Klebsiella variicola FH-1 could degrade atrazine herbicides, and the vegetative growth of atrazine-sensitive crops (i.e., soybean) was significantly increased in the FH-1-treated soil. Interestingly, we found that FH-1 could promote soybean growth and induce resistance to S. sclerotiorum. In our study, strain FH-1 could grow in a nitrogen-free environment, dissolve inorganic phosphorus and potassium, and produce indoleacetic acid and a siderophore. The results of pot experiments showed that K. variicola FH-1 promoted soybean plant development, substantially improving plant height, fresh weight, and root length, and induced resistance against S. sclerotiorum infection in soybean leaves. The area under the disease progression curve (AUDPC) for treatment with strain FH-1 was significantly lower than the control and was reduced by up to 42.2% within 48 h (P<0.001). Moreover, strain FH-1 rcovered the activities of catalase, superoxide dismutase, peroxidase, phenylalanine ammonia lyase, and polyphenol oxidase, which are involved in plant protection, and reduced malondialdehyde accumulation in the leaves. The mechanism of induction of resistance appeared to be primarily resulted from the enhancement of transcript levels of PR10, PR12, AOS, CHS, and PDF1.2 genes. The colonization of FH-1 on soybean root, determined using CLSM and SEM, revealed that FH-1 colonized soybean root surfaces, root hairs, and exodermis to form biofilms. In summary, K. variicola FH-1 exhibited the biological control potential by inducing resistance in soybean against S. sclerotiorum infection, providing new suggestions for green prevention and control.

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A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development

WANG Li-xin, WANG Lin-xia, ZHANG Meng-ling, QU Ying-yue, YUAN Ye, Ehsan SADEGHNEZHAD, GAO Meng-jiao, ZHAO Ruo-yu, QI Chao-feng, GUO Xiao-xue, ZHU Wen-hui, LI Rui-mei, DAI Li, LIU Meng-jun, LIU Zhi-guo

2023, 22 (7): 2094-2110. DOI: 10.1016/j.jia.2023.04.039

Abstract （179） PDF in ScienceDirect

3´,5´-Cyclic adenosine monophosphate (cAMP) is an important metabolite that is specifically enriched in jujube. However, the effect of cAMP on jujube cellular responses has not been comprehensively studied. Here, we established jujube cell suspension cultures and investigated the calcium influx in response to cAMP treatment through protoplast isolation and fluorescence intensity. Firstly, cAMP treatment could promote jujube growth and increase the content of endogenous cAMP. Using transcriptome analysis with transgenic Arabidopsis plants overexpressing adenylate cyclase (ZjAC) as a positive control, we identified 60 calcium-related differential expressed genes (DEGs) that contributed to the calcium signaling and inter- or intra-cellular responses. Pharmacological treatments such as cAMP and the calcium ionophore A23187 could induce ZjAC expression, the accumulation of cAMP and calcium influx in jujube cells, while ethylene glycol tetraacetic acid (EGTA) or bithionol treatment inhibited these changes. Moreover, the calcium channels and transporters in calcium influx, such as the ZjCNGC2 channel and the mitogen activated protein (MAP) kinase pathway, could be activated by cAMP treatment. In summary, our findings demonstrated that cAMP biosynthesis is dependent on calcium influx and the amplifying effect between calcium and cAMP may be involved in intracellular signal induction, which might contribute to the growth and development of jujube.

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Genetic diversity analysis and GWAS reveal the adaptive loci of milling and appearance quality of japonica (oryza sativa L.) in Northeast China

XU Xin, YE Jun-hua, YANG Ying-ying, LI Ruo-si, LI Zhen, WANG Shan, SUN Yan-fei, ZHANG Meng-chen, XU Qun, FENG Yue, WEI Xing-hua, YANG Yao-long

2022, 21 (6): 1539-1550. DOI: 10.1016/S2095-3119(21)63701-2

Abstract （387） PDF in ScienceDirect

Milling and appearance quality are important contributors to rice grain quality. Abundant genetic diversity and a suitable environment are crucial for rice improvement. In this study, we investigated the milling and appearance quality-related traits in a panel of 200 japonica rice cultivars selected from Liaoning, Jilin and Heilongjiang provinces in Northeast China. Pedigree assessment and genetic diversity analysis indicated that cultivars from Jilin harbored the highest genetic diversity among the three geographic regions. An evaluation of grain quality indicated that cultivars from Liaoning showed superior milling quality, whereas cultivars from Heilongjiang tended to exhibit superior appearance quality. Single- and multi-locus genome-wide association studies (GWAS) were conducted to identify loci associated with milling and appearance quality-related traits. Ninety-nine significant single-nucleotide polymorphisms (SNPs) were detected. Three common SNPs were detected using the mixed linear model (MLM), mrMLM, and FASTmrMLM methods. Linkage disequilibrium decay was estimated and indicated three candidate regions (qBRR-1, qBRR-9 and qDEC-3) for further candidate gene analysis. More than 300 genes were located in these candidate regions. Gene Ontology (GO) analysis was performed to discover the potential candidate genes. Genetic diversity analysis of the candidate regions revealed that qBRR-9 may have been subject to strong selection during breeding. These results provide information that will be valuable for the improvement of grain quality in rice breeding.

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The use of widely targeted metabolite profiling to reveal the senescence changes in postharvest ‘Red Globe’ (Vitis vinifera) grape berries

XU Teng-fei, YANG Xin, ZHANG Meng, GUO Shui-huan, FU Wen-jing, ZHOU Bi-jiang, LIU Yu-jia, MA Hai-jun, FANG Yu-lin, YANG Gang, MENG Jiang-fei

2022, 21 (4): 1028-1043. DOI: 10.1016/S2095-3119(21)63725-5

Abstract （159） PDF in ScienceDirect

Changes in the metabolites of table grapes (Vitis vinifera) reportedly occur during postharvest senescence. The aim of this study was to determine the metabolomic differences in postharvest table grapes (‘Red Globe’) after being subjected to different senescence periods. To this end, we used widely targeted metabolomics based on ultra-performance liquid chromatography and tandem mass spectrometry. A total of 135 differential metabolites were identified. During postharvest senescence, the levels of most differential flavonoids (e.g., pelargonidin 3-O-glucoside, quercetin-3-O-glucoside, and cyanidin 3-O-glucoside) and L-aspartic acid decreased, while the levels of phenolic acids (e.g., trans-4-hydroxycinnamic acid methyl ester) and pantothenol increased. During early and late senescence, the levels of most differential lipids, especially LysoPC, as well as those of nucleotides and their derivatives, such as uridine, decreased and increased, respectively. Collectively, the findings of this study provide fundamental insights into the reasonable control of table grape fruit postharvest senescence and lay a solid foundation for further research.

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A sensitive SYBR Green RT-qPCR method for grapevine virus E and its application for virus detection in different grapevine sample types

REN Fang, ZHANG Zun-ping, FAN Xu-dong, HU Guo-jun, ZHANG Meng-yan, DONG Ya-feng

2020, 19 (7): 1834-1841. DOI: 10.1016/S2095-3119(19)62784-X

Abstract （137） PDF in ScienceDirect

To develop a rapid and high-sensitivity method for detection of grapevine virus E (GVE), a SYBR Green based real-time fluorescence quantitative RT-PCR method (RT-qPCR) was established. This method could be used to detect GVE specifically, and the sensitivity was about 100 times greater than conventional RT-PCR. An excellent linear correlation (R²=0.997) and a high amplification efficiency (E=97.5%) were obtained from the standard curve of this method. Reproducibility tests revealed that the coefficients of variation in the intra- and inter-assay results were 0.31–1.03% and 0.82–2.62%, respectively, indicating a good reproducibility. The RT-qPCR method could be used to detect GVE in a wide range of grapevine sample types. The detection rates of RT-qPCR for nearly all sample types from different positions and seasons were higher than conventional RT-PCR. The detection rates in spring, summer, autumn and winter increased gradually. Samples in autumn and winter were best for detection, and the detection rates of most samples were 80–100%, which were 10 to 40% higher than conventional RT-PCR. In general, old petioles and branches were the best tissues for GVE detection. The detection rates of these samples in each season were all 100%, which were 20 to 40% higher than conventional RT-PCR. The second highest rates were in the old leaf, with detection rates for RT-qPCR of 80–100% in all seasons, which were 20 to 40% higher than conventional RT-PCR. GVE could be difficultly detected in young leaves by conventional RT-PCR, and the detection rates were only 0–50%, while by RT-qPCR the rates could increase to 0–80%. A total of 33 out of 363 samples (belonging to 68 cultivars) from 20 regions in China were detected to be positive by RT-qPCR (9.1%), which was more than twice the rate of the conventional RT-PCR (3.9%).

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Prevalence and genetic diversity of grapevine fabavirus isolates from different grapevine cultivars and regions in China

FAN Xu-dong, ZHANG meng-yan, ZHANG Zun-ping, REN Fang, HU Guo-jun, DONG Ya-feng

2020, 19 (3): 768-774. DOI: 10.1016/S2095-3119(19)62677-8

Abstract （129） PDF in ScienceDirect

A total of 288 grapevine samples of 61 different grapevine cultivars, collected from 22 provinces and regions, were analyzed by reverse transcription-PCR (RT-PCR) for the presence of grapevine fabavirus (GFabV). PCR detection results showed the incidences of GFabV were 12.8% (30/235) and 48.1% (25/52) in the asymptomatic and symptomatic vines, respectively. The genetic diversity of GFabV isolates was analyzed based on partial nucleotide and encoded amino acid sequences of the RNA1 and RNA2 polyprotein genes. Phylogenetic analyses of the RNA1 and RNA2 gene sequences divided the GFabV isolates into five well-defined groups. Groups 1, 2, and 4 comprised only Chinese isolates. This article represents the first report for the prevalence and genetic diversity of GFabV in grapevines grown in China.

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Investigation on the co-infections of Toxoplasma gondii with PRRSV, CSFV or PCV-2 in swine in part of China

Wang Shuai, ZHang Meng, LIU Xin-chao, LIn Tao, Yang Han-chun, YUan Shi-shan, ZHao guang-wei, Ia Hassan, Yan Ruo-feng, Song Xiao-kai, XU Li-xin, LI Xiang-rui

2015, 14 (9): 1838-1844. DOI: 10.1016/S2095-3119(15)61044-9

Abstract （1515） PDF in ScienceDirect

The objective of the present investigation was to estimate the prevalence of Toxoplasma gondii infection and co-infection with porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV) and porcine circovirus type 2 (PCV-2) in pigs in China. A total of 372 tissues or serum samples collected from pigs distributed in 9 provinces/ municipalities of China during the period from February 2011 to November 2012 were assayed for T. gondii antigens and antibodies using enzyme linked immunosorbent assay (ELISA) technique, while the PCR was designed for the detection of the PRRSV, CSFV and PCV-2, respectively. The total positive rate of T. gondii, PRSSV, CSFV and PCV-2 was 9.14% (34/372), 50.00% (186/372), 37.10% (138/372) and 3.23% (12/372), respectively. Among the 34 T. gondii positive samples, 26 samples were simultaneously infected with T. gondii and viruses, while the remaining eight samples were infected with T. gondii alone. In addition, the co-infection rate of T. gondii with PRSSV, T. gondii with PRSSV and CSFV, T. gondii with PRSSV and PCV-2, T. gondii with CSFV and PCV-2, T. gondii with PRSSV, CSFV and PCV-2 was 1.61% (6/372), 4.03% (15/372), 0.27% (1/372), 0.27% (1/372) and 0.81% (3/372), respectively. The results of the present survey revealed that PRRSV and CSFV were the common pathogens co-existing with porcine toxoplasmosis in China, and both of them could increase the chances of T. gondii infection in pig. This is the first report of T. gondii co-infections with viruses in pigs. It is very important to understand the interactions of parasite and virus, and can be used as reference data for the control and prevention of co-infections of T. gondii and viruses in pigs.

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Type I strain of Toxoplasma gondii from chicken induced different immune responses with that from human, cat and swine in chicken

Zhao Guang-wei, WanG Shuai, WanG Wang, ZhanG Zhen-chao, XIe Qing, ZhanG Meng, I a hassan, Yan Ruo-feng, SonG Xiao-kai, Xu Li-xin, LI Xiang-rui

2015, 14 (5): 956-965. DOI: 10.1016/S2095-3119(14)60861-3

Abstract （2216） PDF in ScienceDirect

In this study, four strains of Toxoplasma gondii with the same genetic type (Type I) originated from chicken, human, cat and swine were used to compare the immune responses in resistant chicken host to investigate the relationships between the parasite origins and the pathogenicity in certain host. A total of 300, 10-day-old chickens were allocated randomly into five groups which named JS (from chicken), CAT (from cat), CN (from swine), RH (from human) and a negative control group (–Ve) with 60 birds in each group. Tachyzoites of four different T. gondii strains (JS, CAT, CN and RH) were inoculated intraperitoneally with the dose of 1×107 in the four designed groups, respectively. The negative control (–Ve) group was mockly inoculated with phosphate-buffered saline (PBS) alone. Blood and spleen samples were obtained on the day of inoculation (day 0) and at days 4, 11, 25, 39 and 53 post-infection to screen the immunopathological changes. The results demonstrated some different immune characters of T. gondii infected chickens with that of mice or swine previous reported. These differences included up-regulation of major histocompatibility complex class II (MHC II) molecules in the early stage of infection, early peak expressions of interleukin (IL)-12 (IL-12) and -10 (IL-10) and long keep of IL-17. These might partially contribute to the resistance of chicken to T. gondii infection. Comparisons to chickens infected with strains from human, cat and swine, chickens infected with strain from chicken showed significant high levels of CD4+ and CD8+ T cells, interferon gamma (IFN-γ), IL-12 and IL-10. It suggested that the strain from chicken had different ability to stimulate cellular immunity in chicken.

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18S ribosomal RNA methyltransferase METTL5-mediated CDX2 translation regulates porcine early embryo development

XU Teng-teng, ZHANG Meng-ya, LIU Qiu-chen, WANG Xin, LUO Peng-fei, LIU Tong, YAN Ye-lian, ZHOU Na-ru, MA Yang-yang, YU Tong, LI Yun-sheng, CAO Zu-bing, ZHANG Yun-hai

DOI: 10.1016/j.jia.2023.10.013 Online: 19 October 2023

Abstract （96） PDF in ScienceDirect

N6-methyladenosine (m6A) plays a key role in mammalian early embryonic development and cell lineage differentiation. However, the role and mechanisms of 18S ribosomal RNA (rRNA) m6A methyltransferase METTL5 in early embryonic development remain unclear. Here, we found that 18S rRNA m6A methyltransferase METTL5 plays an important role in porcine early embryonic development. METTL5 knockdown and overexpression significantly reduced the developmental efficiency of porcine early embryos and impaired cell lineage allocation. METTL5 knockdown apparently decreased the global translation efficiency in blastocyst, while METTL5 overexpression increased the global translation efficiency. Furthermore, METTL5 knockdown did not affect the abundance of CDX2 mRNA, but resulted in a significant reduction in CDX2 protein levels. Moreover, the low developmental efficiency and abnormal lineage distribution of METTL5 knockdown embryos could be rescued by CDX2 overexpression. Collectively, our results demonstrated that 18S rRNA methyltransferase METTL5 regulates porcine early embryonic development via modulating the translation of CDX2.

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MiR-21-5p ameliorates Busulfan-induced testicular dysfunction and maintains spermatogenesis

ZHANG Meng-fei, WAN Shi-cheng, CHEN Wen-bo, YANG Dong-hui, WANG Cong-liang, LI Ba-lun, Aili Aierken, DU Xiao-min, LI Yun-xiang, WU Wen-ping, WANG Yu-qi, XIE Fang-de, LUO Xuan, LI Na, LI Xue-ling, YANG Lei, Ahmed Hamed Arisha, HUA Jin-lian

DOI: 10.1016/j.jia.2024.02.004 Online: 07 March 2024

Abstract （103） PDF in ScienceDirect

Spermatogonial stem cells (SSCs) are the key to maintaining production of the sperms and healthy offsprings, and also treating breeding livestock’s reproductive damage and infertility. MicroRNAs act a decisive role in regulating gene expression in many cells and tissues, including in processes such as proliferation, self-renewal, differentiation, and apoptosis of stem cells. However, the miRNA mechanism in regulation of SSCs is still unclear. Here, high-throughput sequencing was used to identify specific miRNAs. We confirmed that miR-21-5p was concentrated in both goat and mouse SSCs, and enhanced the proliferation and antiapoptotic ability of SSCs. In vivo experiments have shown that miR-21-5p resisted the damage of the chemotherapy drug Busulfan to germ cells, ameliorated Busulfan-induced testicular dysfunction, and maintained spermatogenesis. Further RNA-seq and target gene prediction revealed that SPRY1 and FASLG are targets of miR-21-5p, thereby activating downstream signaling pathways such as MAPK/ERK, PI3K-AKT, and apoptosis. In summary, miR-21-5p is crucial for the self-renewal and maintenance of SSCs. This study provides new avenues for treating breeding livestock’s reproductive damages, infertility, oligospermia, and other conditions.

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