Tick-borne encephalitis (TBE) is an important zoonotic viral disease transmitted by ticks. In recent decades, global climate change has increased human exposure to ticks, and mortality rates have gradually risen. Effective vaccines are essential for controlling TBE as specific antiviral treatment is unavailable. Vaccine candidates based on virus-like particles (VLPs) have previously been demonstrated to be efficient in eliciting excellent immune responses against influenza virus and SARS-CoV-2. Here, we constructed TBE virus (TBEV) VLPs containing the envelope and membrane proteins derived from the Far Eastern TBEV strain (WH2012) using an insect cell-baculovirus expression system. Induction of immune responses was investigated in mice following intramuscular injection with the TBEV VLPs vaccine candidates formulated of Poly(I:C) & Montanide ISA 201VG combination adjuvants. Mice produced memory T-cells and serum-specific IgG antibodies that averaged up to 1:10^4.6 and remained at 1:10⁴ (mean) for 24 weeks after three immunizations. TBEV VLPs vaccine was able to provide long-term antibody protection against TBEV, making it a promising subunit vaccine candidate for this disease.

Rapeseed (Brassica napus L.) is one of the most important oilseed crops worldwide.  Development of rapeseed varieties with high-quality oil is a long-term breeding goal.  Reducing the contents of palmitic acid, the main saturated fatty acid in rapeseed oil, could greatly improve oil quality.  Here, we performed genome-wide association study (GWAS) and transcriptome-wide association study (TWAS) of seed palmitic acid content (SPAC) using 393 diverse B. napus accessions.  Four genes (BnaA08.DAP, BnaA08.PAA1, BnaA08. DUF106, and BnaC03.DAP) were identified by both GWAS and TWAS.  The transcripts per million (TPM) values of these candidate genes at 20 and 40 days after flowering (DAF) were significantly correlated with SPAC in this association panel.  Based on genetic variation in the candidate genes, we identified four low-SPAC haplotypes by combining candidate gene association analysis and haplotype analysis.  Brassica napus accessions carrying low-SPAC haplotypes had lower SPAC than those carrying high-SPAC haplotypes without affecting seed oil content, seed protein content, or seed yield.  Based on the functional single-nucleotide polymorphism (SNP) chrA08_9529850 (C/A) in the promoter of BnaA08.DUF106, we developed a molecular marker (Bn_A8_SPAC_Marker) that could be used to facilitate breeding for low SPAC in B. napus.  Our findings provide valuable information for studying the genetic control of SPAC in B. napus.  Moreover, the candidate genes, favorable haplotypes, and molecular marker identified in this study will be useful for breeding low-SPAC B. napus varieties.