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Irrigation and nitrogen fertiliser optimisation in protected vegetable fields of northern China: Achieving environmental and agronomic sustainability

Bingqian Fan, Yitao Zhang, Owen Fenton, Karen Daly, Jungai Li, Hongyuan Wang, Limei Zhai, Xiaosheng Luo, Qiuliang Lei, Shuxia Wu, Hongbin Liu
2024, 23 (3): 1022-1033.   DOI: 10.1016/j.jia.2023.12.019
Abstract123)      PDF in ScienceDirect      

Globally, sub-optimal use of nitrogen (N) fertiliser and elevated N irrigation groundwater have led to high leached nitrate (NO3) losses from protected vegetable field systems.  Optimising fertiliser and irrigation management in different soil types is crucial to reduce future N loads from such systems.  The present 4-year study examined leached N loads from lysimeter monitoring arrays set up across 18 protected vegetable system sites encompassing the dominant soil types of northern China.  The treatments applied at each field site were: 1) a high N and high irrigation input treatment (HNHI); 2) a low N but high irrigation input treatment (LNHI) and 3) a low N with low irrigation input treatment (LNLI).  Results showed that the mean annual leached total nitrogen loads from the HNHI, LNHI and LNLI treatments were 325, 294 and 257 kg N ha–1 in the fluvo-aquic soil, 114, 100 and 78 kg N ha–1 in the cinnamon soil and 79, 68 and 57 kg N ha–1 in the black soil, respectively.  The N dissolved in irrigation water in the fluvo-aquic soil areas was 8.26-fold higher than in the cinnamon areas.  A structural equation model showed that N fertiliser inputs and leaching water amounts explained 14.7 and 81.8% of the variation of leached N loads, respectively.  Correspondingly, reducing irrigation water by 21.5% decreased leached N loads by 20.9%, while reducing manure N and chemical N inputs by 22 and 25% decreased leached N loads by only 9.5%.  This study highlights that protected vegetable fields dominated by fluvo-aquic soil need management to curtail leached N losses in northern China.

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The shaping of milk-flavored white tea: More than a change in appearance
Jiao Feng, Weisu Tian, Jinyuan Wang, Shuping Ye, Guanjun Pan, Bugui Yu, Fang Wang, Hongzheng Lin, Zhilong Hao
2024, 23 (11): 3912-3922.   DOI: 10.1016/j.jia.2024.09.010
Abstract64)      PDF in ScienceDirect      
Tea’s popularity and flavor are influenced by factors like cultivation and processing methods and shaping techniques also have an impact on tea flavor.  This study employed targeted metabolomics and chemometrics to investigate how shaping techniques affect the flavor of milk-flavored white tea (MFWT).  The results showed that the tea cake sample with the shortest pressing time (Y90) has the highest amino acid content and milky aroma intensity.  There were variations in amino acids, catechins, and soluble sugars among MFWT samples with different shaping techniques.  The total contents of amino acids and catechins in tea cake sample (Y90) were significantly lower than those in the loose tea sample (SC) and bundle-like tea sample (SG), while the total sugar content was significantly higher than that in SC (P<0.05).  Additionally, the content of volatiles presenting milky aroma (VIP&OAV>1) in Y90 remained lower relative to SC and SG (P<0.05), but the proportion was not different from that in SC and SG, minimally affecting the overall flavor.  The short-time pressing method might be suitable for mass production of MFWT.  These findings provide insights into improving the tightness of the appearance of MFWT with minimal impact on tea flavor.


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GbLMI1 over-expression improves cotton aboveground vegetative growth
Zhili Chong, Yunxiao Wei, Kaili Li, Muhammad Aneeq Ur Rahman, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Liangrong He, Rui Zhang
2024, 23 (10): 3457-3467.   DOI: 10.1016/j.jia.2023.05.037
Abstract131)      PDF in ScienceDirect      
Leaves are the main organ for photosynthesis and organic synthesis in cotton.  Leaf shape has important effects on photosynthetic efficiency and canopy formation, thereby affecting cotton yield.  Previous studies have shown that LMI1 (LATE MERISTEM IDENTITY1) is the main gene regulating leaf shape.  In this study, the LMI1 gene was inserted into the 35S promoter expression vector, and cotton plants overexpressing LMI1 (OE) were obtained through genetic transformation.  Statistical analysis of the biological traits of the T1 and T2 populations showed that compared to the wild type (WT), OE plants had significantly larger leaves, thicker stems and significantly greater dry weight.  Furthermore, plant sections of the main vein and petiole showed that the numbers of cells in those tissues of OE plants were significantly greater.  In addition, RNA-seq analysis revealed the differential expression of genes related to gibberellin synthesis and NAC gene family (genes containing the NAC domain) between the OE and WT plants, suggesting that LMI1 is involved in secondary wall formation and cell proliferation, which promotes stem thickening.  Moreover, Gene Ontology (GO) analysis revealed enrichment in the terms of calcium ion binding, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed enrichment in the terms of fatty acid degradation, phosphatidylinositol signal transduction system, and cAMP (cyclic adenosine monophosphate) signal pathway.  These results suggested that LMI1 OE plants are responsive to gibberellin hormone signals, and have altered messenger signals (cAMP, Ca2+) which amplify this function, to promote stronger aboveground vegetative growth.  This study found the LMI1 greatly increased the vegetative growth in cotton, which is the basic requirement for higher yield.


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Research on grain supply and demand matching in the Beijing–Tianjin–Hebei Region based on ecosystem service flows
Jiaxin Miao, Peipei Pan, Bingyu Liu, XiaowenYuan, Zijun Pan, Linsi Li, Xinyun Wang, Yuan Wang, Yongqiang Cao, Tianyuan Zhang
DOI: 10.1016/j.jia.2025.04.024 Online: 22 April 2025
Abstract4)      PDF in ScienceDirect      

A comprehensive assessment of grain supply, demand, and ecosystem service flows is essential for identifying grain movement pathways, ensuring regional grain security, and guiding sustainable management strategies. However, current studies primarily focus on short-term grain provision services while neglecting the spatiotemporal variations in grain flows across different scales. This gap limits the identification of dynamic matching relationships and the formulation of optimization strategies for balancing grain flows. This study examined the spatiotemporal evolution of grain supply and demand in the Beijing–Tianjin–Hebei (BTH) region from 1980 to 2020. Using the Enhanced Two-Step Floating Catchment Area method, the grain provision ecosystem service flows were quantified, the changes in supply–demand matching under different flow scenarios were analyzed and the optimal distance threshold for grain flows was investigated. The results revealed that grain production follows a spatial distribution pattern characterized by high levels in the southeast and low levels in the northwest. A significant mismatch exists between supply and demand, and it shows a scale effect. Deficit areas are mainly concentrated in the northwest, while surplus areas are mainly located in the central and southern regions. As the spatial scale increases, the ecosystem service supply–demand ratio (SDR) classification becomes more clustered, while it exhibits greater spatial SDR heterogeneity at smaller scales. This study examined two distinct scenarios of grain provision ecosystem service flow dynamics based on 100 km and 200 km distance thresholds. The flow increased significantly, from 2.17 to 11.81 million tons in the first scenario and from 2.41 to 12.37 million tons in the second scenario over nearly 40 years, forming a spatial movement pattern from the central and southern regions to the surrounding areas. Large flows were mainly concentrated in the interior of urban centers, with significant outflows between cities such as Baoding, Shijiazhuang, Xingtai, and Hengshui. At the county scale, supply–demand matching patterns remained consistent between the grain flows in the two scenarios. Notably, incorporating grain flow dynamics significantly reduced the number of grain-deficit areas compared to scenarios without grain flowIn 2020, grain-deficit counties decreased by 28.79% and 37.88%, and cities by 12.50% and 25.0% under the two scenarios, respectively. Furthermore, the distance threshold for achieving optimal supply and demand matching at the county scale was longer than at the city scale in both flow scenarios. This study provides valuable insights into the dynamic relationships and heterogeneous patterns of grain matching, and expands the research perspective on grain and ecosystem service flows across various spatiotemporal scales.

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ZmCals12 impacts the maize growth and development by regulating symplastic transport
Ziwen Shi, Sheng Zhang, Qing He, Xiaoyuan Wang, Bo yang, Tao Yu, Hongyang Yi, Tingzhao Rong, Moju Cao
DOI: 10.1016/j.jia.2024.05.010 Online: 29 May 2024
Abstract34)      PDF in ScienceDirect      
Carbohydrate partitioning from source to sink tissues is critical for plant growth and development. However, in maize (Zea mays L.), the molecular mechanisms of callose synthase gene regulating this process have seldom been reported.  Here, we show that the mutation of maize callose synthase12 (ZmCals12) caused increased accumulation of carbohydrate in the photosynthetic leaves but reduced carbohydrate content in sink tissues, which led to plant dwarfing and male sterile.  Histochemical GUS activity assay and mRNA in situ hybridization (ISH) indicated that ZmCals12 was mainly expressed in the vascular transport system.  Loss-of-function of ZmCals12 reduced callose synthase activity and callose deposition in plasmodesmatas (PDs) and around phloem cells (PCs) of vascular bundle.  The drop-and-see (DANS) assay revealed that the PD permeability in the photosynthetic cells and the transport competence of leaf veins were reduced in the Zmcals12 mutants, which led to the reduced symplastic transport.  Paraffin section experiment revealed that less-developed vascular cells (VCs) in the Zmcals12 mutants potentially disturbed sugar transport, thus resulting in the pleiotropic phenotype of the Zmcals12 mutants.  In addition, the impaired sugar transport hindered the internode development by inhibiting auxin (IAA) biosynthesis and signaling in the Zmcals12 mutant.  Collectively, our results provide insights into the mechanism of ZmCals12-mediated callose deposition and symplastic transport governing maize growth and development.
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