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Model fitting of the seasonal population dynamics of the soybean aphid, Aphis glycines Matsumura, in the field

XU Lei, ZHAO Tong-hua, Xing Xing, XU Guo-qing, XU Biao, ZHAO Ji-qiu
2023, 22 (6): 1797-1808.   DOI: 10.1016/j.jia.2023.02.021
Abstract179)      PDF in ScienceDirect      

The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is one of the greatest threats to soybean production, and both trend analysis and periodic analysis of its population dynamics are important for integrated pest management (IPM).  Based on systematically investigating soybean aphid populations in the field from 2018 to 2020, this study adopted the inverse logistic model for the first time, and combined it with the classical logistic model to describe the changes in seasonal population abundance from colonization to extinction in the field.  Then, the increasing and decreasing phases of the population fluctuation were divided by calculating the inflection points of the models, which exhibited distinct seasonal trends of the soybean aphid populations in each year.  In addition, multifactor logistic models were then established for the first time, in which the abundance of soybean aphids in the field changed with time and relevant environmental conditions.  This model enabled the prediction of instantaneous aphid abundance at a given time based on relevant meteorological data.  Taken as a whole, the successful approaches implemented in this study could be used to build a theoretical framework for practical IPM strategies for controlling soybean aphids.

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Grain yield and nitrogen use efficiency of an ultrashort-duration variety grown under different nitrogen and seeding rates in direct-seeded and double-season rice in Central China
WANG Xin-yu, YANG Guo-dong, XU Le, XIANG Hong-shun, YANG Chen, WANG Fei, PENG Shao-bing
2023, 22 (4): 1009-1020.   DOI: 10.1016/j.jia.2022.08.019
Abstract277)      PDF in ScienceDirect      

Nitrogen (N) and seeding rates are important factors affecting grain yield and N use efficiency (NUE) in direct-seeded rice.  However, these factors have not been adequately investigated on direct-seeded and double-season rice (DDR) in Central China.  The objective of this study was to evaluate the effects of various N and seeding rates on the grain yield and NUE of an ultrashort-duration variety grown under DDR.  Field experiments were conducted in 2018 in Wuxue County and 2019 in Qichun County, Hubei Province, China with four N rates and three seeding rates.  The results showed that the grain yield of the ultrashort-duration variety ranged from 6.32 to 8.23 t ha–1 with a total growth duration of 85 to 97 days across all treatments with N application.  Grain yield was increased significantly by N application in most cases, but seeding rate had an inconsistent effect on grain yield.  Furthermore, the response of grain yield to the N rates was much higher than the response to seeding rates.  The moderate N rates of 100–150 and 70–120 kg N ha–1 in the early and late seasons, respectively, could fully express the yield potential of the ultrashort-duration variety grown under DDR.  Remarkably higher N responses and agronomic NUE levels were achieved in the early-season rice compared with the late-season rice due to the difference in indigenous soil N supply capacity (INS) between the two seasons.  Seasonal differences in INS and N response should be considered when crop management practices are optimized for achieving high grain yield and NUE in ultrashort-duration variety grown under DDR.

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Grain yield and lodging-related traits of ultrashort-duration varieties for direct-seeded and double-season rice in Central China
WANG Xin-yu, XU Le, LI Xiao-xiao, YANG Guo-dong, WANG Fei, PENG Shao-bing
2022, 21 (10): 2888-2899.   DOI: 10.1016/j.jia.2022.07.035
Abstract204)      PDF in ScienceDirect      
Lodging is the most common constraint on grain yield of direct-seeded rice.  There is limited information about lodging resistance and its related plant traits in direct-seeded and double-season rice (DDR) in Central China.  This study aims  to identify the plant traits that achieve high lodging resistance in ultrashort-duration varieties (about 95 days) of DDR.  Field experiments were conducted in 2017 and 2018 in Wuxue County, Hubei Province, China, with four ultrashort-duration varieties grown under two nitrogen (N) rates.  Lodging-related traits were measured on the 15th day after heading, and yield and yield attributes were measured at maturity.  The grain yield of the four varieties ranged from 4.59 to 7.61 t ha–1 across the two N rates, with a total growth duration of 85 to 97 days.  Varietal differences in lodging index were mainly explained by the bending moment, which was closely related to plant height.  Breaking resistance did not affect the lodging index significantly.  Shortening plant height from 95.4 to 80.5 cm decreased the lodging index by 22.4% but did not reduce grain yield.  Our results suggested that reducing plant height was effective in improving the lodging resistance of ultrashort-duration varieties of DDR.  Lodging resistance should be enhanced by improving breaking resistance rather than reducing plant height to increase DDR grain yield further.
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OsHemA gene, encoding glutamyl-tRNA reductase (GluTR) is essential for chlorophyll biosynthesis in rice (Oryza sativa)
ZENG Zhao-qiong, LIN Tian-zi, ZHAO Jie-yu, ZHENG Tian-hui, XU Le-feng, WANG Yi-hua, LIU Ling-long, JIANG Ling, CHEN Sai-hua, WAN Jian-min
2020, 19 (3): 612-623.   DOI: 10.1016/S2095-3119(19)62710-3
Abstract142)      PDF in ScienceDirect      
Chlorophyll (Chl) biosynthesis is essential for photosynthesis and plant growth.  Glutamyl-tRNA reductase (GluTR) catalyzes glutamyl-tRNA into glutamate-1-semialdehyde (GSA) and initiates the chlorophyll biosynthesis.  Even though the main role of GluTR has been established, the effects caused by natural variations in its corresponding gene remain largely unknown.  Here, we characterized a spontaneous mutant in paddy field with Chl biosynthesis deficiency, designated as cbd1.  With intact thylakoid lamellar structure, the cbd1 plant showed light green leaves and reduced Chl and carotenoids (Cars) content significantly compared to the wild type.  By map-based gene cloning, the mutation was restricted within a 57-kb region on chromosome 10, in which an mPingA miniature inverted-repeat transposable element (MITE) inserted in the promoter region of OsHemA gene.  Both leaf color and the pigment contents in cbd1 were recovered in a complementation test, confirming OsHemA was responsible for the mutant phenotype.  OsHemA was uniquely predicted to encode GluTR and its expression level was dramatically repressed in cbd1.  Transient transformation in protoplasts demonstrated that GluTR localized in chloroplasts and a signal peptide exists in its N-terminus.  A majority of Chl biosynthesis genes, except for POR and CHLG, were down-regulated synchronously by the repression of OsHemA, suggesting that an attenuation occurred in the Chl biosynthesis pathway.  Interestingly, we found major agronomic traits involved in rice yield were statistically unaffected, except for the number of full grains per panicle was increased in cbd1.  Collectively, OsHemA plays an essential role in Chl biosynthesis in rice and its weak allele can adjust leaf color and Chls content without compromise to rice yield.
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Allelochemical p-hydroxybenzoic acid inhibits root growth via regulating ROS accumulation in cucumber (Cucumis sativus L.)
HUANG Cheng-zhen, XU Lei, Sun Jin-jing, ZHANG Zhong-hua, FU Mei-lan, TENG Hui-ying, YI Ke-ke
2020, 19 (2): 518-527.   DOI: 10.1016/S2095-3119(19)62781-4
Abstract90)      PDF in ScienceDirect      
Allelopathy is prevalent in agricultural ecosystems and mediated by plant-derived secondary metabolites (allelochemicals).  Allelochemicals are released by donor plants and affect the root growth and development of receptor plants.  Allelopathy is responsible for the continuous cropping obstacles in cucumber (Cucumis sativus L.).  p-Hydroxybenzoic acid (pHBA), an autotoxin from root exudates of cucumber, has been proposed to be an important allelopathic chemical.  However, the molecular mechanism by which pHBA affect root growth and development in cucumber is unknown.  Here, we found that pHBA treatment suppressed root growth of cucumber by reducing the meristem activity and cell length.  This root growth defect is caused by reduced reactive oxygen species (ROS) accumulation in root tips.  After pHBA treatment, the expression levels of several ROS-scavenging-related genes were increased, including peroxidase (POD), catalase (CAT) and metallothionein (MT).  Moreover, exogenously application of salicylhydroxamate (SHAM), a peroxidase inhibitor, can partially restore the pHBA treatment induced root growth inhibition.  Furthermore, we found that there is natural variation for the inhibitory effect of pHBA on root growth.  We also showed that pHBA treatment could maintain higher level of ROS accumulated in the pHBA less sensitive cucumber than that in the pHBA-sensitive cucumber.  These results suggest that pHBA inhibits root growth by reducing root tip ROS level in cucumber.
 
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RNA interference in Colorado potato beetle (Leptinotarsa decemlineata): A potential strategy for pest control
MA Mei-qi, HE Wan-wan, XU Shi-jing, XU Le-tian, ZHANG Jiang
2020, 19 (2): 428-427.   DOI: 10.1016/S2095-3119(19)62702-4
Abstract108)      PDF in ScienceDirect      
Colorado potato beetle (CPB), Leptinotarsa decemlineata, is a notorious destructive pest that mainly feeds on the leaves of potato and several other solanaceous plants.  CPB is widely recognized for its adaptation to a remarkable variety of host plants and diverse climates, and its high resistance to insecticides and Bacillus thuringiensis toxins.  RNA interference (RNAi) is a sequence-specific, endogenous gene silencing mechanism evoked by small RNA molecules that is used as a robust tool for virus and pest control.  RNAi has been extensively tested for CPB management by employing various target genes and delivery methods.  This article reviews the screening of RNAi target genes, efficient RNAi delivery systems, and factors affecting RNAi efficiency in CPB, which may help understand the mechanisms of RNAi and its application in CPB control strategy.
 
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Identification of the strain-specifically truncated nonstructural protein 10 of porcine reproductive and respiratory syndrome virus in infected cells
ZHANG Zhi-bang, XU Lei, WEN Xue-xia, DONG Jian-guo, ZHOU Lei, GE Xin-na, YANG Han-chun, GUO Xin
2018, 17 (05): 1171-1180.   DOI: 10.1016/S2095-3119(17)61896-3
Abstract522)      PDF (4491KB)(540)      
The nonstructural protein 10 (nsp10) of porcine reproductive and respiratory syndrome virus (PRRSV) encodes for helicase which plays a vital role in viral replication.  In the present study, a truncated form of nsp10, termed nsp10a, was found in PRRSV-infected cells and the production of nsp10a was strain-specific.  Mass spectrometric analysis and deletion mutagenesis indicated that nsp10a may be short of about 70 amino acids in the N terminus of nsp10.  Further studies by rescuing recombinant viruses showed that the Glu-69 in nsp10 was the key amino acid for nsp10a production.  Finally, we demonstrated that nsp10a exerted little influence on the growth kinetics of PRRSV in vitro. 
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Extreme meteorological disaster effects on grain production in Jilin Province, China
XU Lei, ZHANG Qiao, ZHANG Jing, ZHAO Liang, SUN Wei, JIN Yun-xiang
2017, 16 (02): 486-496.   DOI: 10.1016/S2095-3119(15)61285-0
Abstract1094)      PDF in ScienceDirect      
Extreme meteorological disaster effects on grain production is mainly determined by the interaction between danger degree of hazard-induced factors and vulnerability degree of hazard-affected bodies.  This paper treats physical exposure, sensitivity of the response to the impact, and capabilities of disaster prevention and mitigation as a complex system for vulnerability degree of hazard-affected bodies, which included the external shocks and internal stability mechanism.  Hazard-induced factors generate external shocks on grain production systems though exposure and sensitivity of hazard-affected body, and the result can be represented as affected area of grain.  By quantile regression model, this paper depicts the quantitative relationship between hazard-induced factors of extreme meteorological disaster and the affected area in the tail of the distribution.  Moreover, the model of production function have also been utilized to expound and prove the quantitative relationship between the affected area and final grain output under the internal stability mechanism of the agricultural natural resources endowment, the input factors of agricultural production, and the capacity of defending disaster.  The empirical study of this paper finds that impact effects of drought disaster to grain production system presents the basic law of “diminishing marginal loss”, namely, with the constant improvement of the grade of drought, marginal affected area produced by hazard-induced factors will be diminishing.  Scenario simulation of extreme drought impact shows that by every 1% reduction in summer average rainfall, grain production of Jilin Province will fell 0.2549% and cut production of grain 14.69% eventually.  In response to ensure China’s grain security, the construction of the long-term mechanism of agricultural disaster prevention and mitigation, and the innovation of agricultural risk management tools should be also included in the agricultural policy agenda.
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Assessment of Flood Catastrophe Risk for Grain Production at the Provincial Scale in China Based on the BMM Method
XU Lei, ZHANG Qiao, ZHOU Ai-lian, HUO Ran
2013, 12 (12): 2310-2320.   DOI: 10.1016/S2095-3119(13)60587-0
Abstract1254)      PDF in ScienceDirect      
Flood catastrophe risk assessment is imperative for the steady development of agriculture under the context of global climate change, and meanwhile, it is an urgent scientific issue need to be solved in agricultural risk assessment discipline. This paper developed the methodology of flood catastrophe risk assessment, which can be shown as the standard process of crop loss calculation, Monte Carlo simulation, the generalized extreme value distribution (GEV) fitting, and risk evaluation. Data on crop loss were collected based on hectares covered by natural disasters, hectares affected by natural disasters, and hectares destroyed by natural disasters using the standard equation. Monte Carlo simulation based on appropriate distribution was used to expand sample size to overcome the insufficiency of crop loss data. Block maxima model (BMM) approach based on the extreme value theory was for modeling the generalized extreme value distribution (GEV) of flood catastrophe loss, and then flood catastrophe risk at the provincial scale in China was calculated. The Type III Extreme distribution (Weibull) has a weighted advantage of modeling flood catastrophe risk for grain production. The impact of flood catastrophe to grain production in China was significantly serious, and high or very high risk of flood catastrophe mainly concentrates on the central and eastern regions of China. Given the scenario of suffering once-in-a-century flood disaster, for majority of the major-producing provinces, the probability of 10% reduction of grain output is more than 90%. Especially, the probabilities of more than 15% decline in grain production reach up to 99.99, 99.86, 99.69, and 91.60% respectively in Anhui, Jilin, Liaoning, and Heilongjiang. Flood catastrophe assessment can provide multifaceted information about flood catastrophe risk that can help to guide management of flood catastrophe.
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