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Wheat growth, photosynthesis and physiological characteristics under different soil Zn levels
LI Si-ping, ZENG Lu-sheng, SU Zhong-liang
2022, 21 (
7
): 1927-1940. DOI:
10.1016/S2095-3119(21)63643-2
Abstract
(
262
)
PDF in ScienceDirect
In recent years, heavy metal hazards in the soil have seriously affected agricultural production. This study aims to examine the effects of different levels of heavy metal Zn on the growth, photosynthesis and physiological characteristics of wheat, and provide a theoretical basis for the diagnosis and control of heavy metal pollution in agricultural production. The field test method was used to explore the changes of wheat agronomic traits, photosynthetic capacity, chlorophyll fluorescence parameters, spectral characteristic curve, active oxygen metabolism system, cell ultrastructure, and yield, under different Zn levels (0, 250, 500, 750, and 1 000 mg kg
–1
). The results show that, low-level Zn treatments can effectively promote the synthesis of wheat chlorophyll, improve photosynthetic capacity, and increase yield. The yield of ZnL1 (250 mg kg
–1
) was the highest in the two-year test, which increased by 20.4% in 2018 and 13.9% in 2019 compared with CK (0 mg kg
–1
). However, a high Zn level had a significant stress effect on the photosystem of wheat.
PI
abs
(reaction center performance index) and
F
v
/
F
m
(maximum photochemical efficiency) were significantly reduced, the active oxygen metabolism system was damaged, and the photosynthetic capacity was reduced, which in turn led to reduced yield. Among them, the yield of ZnL4 (1 000 mg kg
–1
) was the lowest in the two-year test, which was 28.1 and 16.4% lower than CK in 2018 and 2019, respectively. The green peak position of ZnL3 and ZnL4 had “red shift” to the long wave direction, while the red valley position of ZnL4 had “blue shift” to the short wave direction. Under ZnL4, some wheat leaf organelles began to decompose, vacuoles increased, cytoplasm decreased, cell walls thickened, chloroplast basal lamellae were disordered, and mitochondrial membranes disintegrated. Stepwise regression and Path analysis showed that
P
n
(net photosynthetic rate) played a leading role in the formation of yield. Redundancy (RDA) analysis showed that the optimal Zn level for wheat growth was about 250 mg kg
–1
, and wheat would be stressed when the soil Zn level exceeded 500 mg kg
–1
in the test condition of this study. Findings of this study provide a theoretical basis for the diagnosis and prevention of heavy metal (Zn) pollution in the soil.
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Identification of eight
Berberis
species from the Yunnan-Guizhou plateau as aecial hosts for
Puccinia striiformis
f. sp.
tritici
, the wheat stripe rust pathogen
LI Si-nan, CHEN Wen, MA Xin-yao, TIAN Xia-xia, LIU Yao, HUANG Li-li, KANG Zhen-sheng, ZHAO Jie
2021, 20 (
6
): 1563-1569. DOI:
10.1016/S2095-3119(20)63327-5
Abstract
(
145
)
PDF in ScienceDirect
Puccinia striiformis
Westend. f. sp.
tritici
Erikss. (
Pst
) infects wheat and causes stripe rust. The rust is heteroecious with wheat as the primary uredinial and telial host and barberry (
Berberis
spp.) as the alternate pycnial and aecial host. More than 40
Berberis
species have been identified as alternate hosts for
Pst
, and most of these are Chinese
Berberis
species. However, little is known about
Berberis
species or their geographic distributions in the Yunnan-Guizhou plateau in southwestern China. The Yunnan-Guizhou plateau is considered to be an important and relatively independent region for the evolution of the wheat stripe rust pathogen in China because the entire disease cycle can be completed within the region. In this study, we conducted a survey of barberry plants in the Yunnan-Guizhou plateau and identified the eight
Pst
-susceptible
Berberis
species under controlled conditions, including
B. julianae
,
B. tsienii
,
B.
veitchii
,
B. wilsonae
,
B. wilsonae
var. guhtzunica,
B. franchetiana
,
B. lepidifolia
and
B. pruinosa
. These species are reported here for the first time to serve as alternate hosts for the wheat stripe rust pathogen under controlled conditions.
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Functional polymorphism among members of abscisic acid receptor family (ZmPYL) in maize
LU Feng-zhong, YU Hao-qiang, LI Si, LI Wan-chen, ZHANG Zhi-yong, FU Feng-ling
2020, 19 (
9
): 2165-2176. DOI:
10.1016/S2095-3119(19)62802-9
Abstract
(
143
)
PDF in ScienceDirect
Pyrabactin resistance 1-like proteins (PYLs) are direct receptors of abscisic acid (ABA). For the redundant and polymorphic functions, some members of the PYL family interact with components of other signaling pathways. Here, 253 positive colonies from a maize cDNA library were screened as interacting proteins with the members of ZmPYL family. After sequencing and function annotation, 17 of 28 interaction combinations were verified by yeast two-hybrid (Y2H). The germination potential, taproot length and proline content of a quartet mutant of
Arabidopsis
PYL
genes were significantly deceased comparing to the wild type (WT) under alkaline stress (pH 8.5) and 100 μmol L
–1
methyl jasmonate (MeJA) induction. The malondialdehyde (MDA) content was significantly increased. After germinating in darkness, the characteristics of dark morphogenesis of the quartet mutant seedlings were more obvious than those of the WT. The differential expression of the related genes of photomorphogenesis in the mutant was much more than that in the WT. Three light and two JA responsive
cis
-affecting elements were identified during the promoter sequences of the
AtPYL1
and
AtPYL2
genes. These results suggested that functional polymorphism has evolved among the members of ZmPYL family. In response to developmental and environmental stimuli, they not only function as direct ABA receptors but also interact with components of other signaling pathways mediated JA, brassinosteroid (BR), auxin, etc., and even directly regulate downstream stress-related proteins. These signaling pathways can interact at various crosstalk points and different levels of gene expression within a sophisticated network.
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Overexpression of
GmBIN2
, a soybean glycogen synthase kinase 3 gene, enhances tolerance to salt and drought in transgenic
Arabidopsis
and soybean hairy roots
WANG Ling-shuang, CHEN Qing-shan, XIN Da-wei, QI Zhao-ming, ZHANG Chao, LI Si-nan, JIN Yang-mei, LI Mo, MEI Hong-yao, SU An-yu, WU Xiao-xia
2018, 17 (
09
): 1959-1971. DOI:
10.1016/S2095-3119(17)61863-X
Abstract
(
530
)
PDF in ScienceDirect
Glycogen synthase kinase 3 (GSK3) is a kind of serine/threonine kinase widely found in eukaryotes. Many plant GSK3 kinases play important roles in regulating stress responses. This study investigated
BRASSINOSTEROID-INSENSITIVE 2
(
GmBIN2
) gene, a member of the GSK3 protein kinase family in soybean and an orthologue of
Arabidopsis
BIN2
/
AtSK21
.
GmBIN2
expression was increased by salt and drought stresses, but was not significantly affected by the ABA treatment. To examine the function of
GmBIN2
, transgenic
Arabidopsis
and transgenic soybean hairy roots were generated. Overexpression of
GmBIN2
in
Arabidopsis
resulted in increased germination rate and root length compared with wild-type plants under salt and mannitol treatments. Overexpression of
GmBIN2
increased cellular Ca
2+
content and reduced Na
+
content, enhancing salt tolerance in transgenic
Arabidopsis
plants. In the soybean hairy root assay, overexpression of
GmBIN2
in transgenic roots also showed significantly higher relative root growth rate than the control when subjected to salt and mannitol treatments. Measurement of physiological indicators, including proline content, superoxide dismutase (SOD) activity, and relative electrical conductivity, supported this conclusion. Furthermore, we also found that
GmBIN2
could up-regulate the expression of some stress-related genes in transgenic
Arabidopsis
and soybean hairy roots. Overall, these results indicated that
GmBIN2
improved tolerance to salt and drought in transgenic
Arabidopsis
and soybean hairy roots.
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Stem flow of seed-maize under alternate furrow irrigation and double-row ridge planting in an arid region of Northwest China
BO Xiao-dong, DU Tai-sheng, DING Ri-sheng, TONG Ling, LI Si-en
2015, 14 (
7
): 1434-1445. DOI:
10.1016/S2095-3119(14)60930-8
Abstract
(
2065
)
PDF in ScienceDirect
Maize is widely planted throughout the world and has the highest yield of all the cereal crops. The arid region of Northwest China has become the largest base for seed-maize production, but water shortage is the bottleneck for its long-term sustainability. Investigating the transpiration of seed-maize plants will offer valuable information for suitable planting and irrigation strategies in this arid area. In this study, stem flow was measured using a heat balance method under alternate furrow irrigation and double-row ridge planting. Meteorological factors, soil water content (θ), soil temperature (Ts) and leaf area (LA) were also monitored during 2012 and 2013. The diurnal stem flow and seasonal dynamics of maize plants in the zones of south side female parent (SFP), north side female parent (NFP) and male parent (MP) were investigated. The order of stem flow rate was: SFP>MP>NFP. The relationships between stem flow and influential factors during three growth stages at different time scales were analyzed. On an hourly scale, solar radiation (Rs) was the main driving factor of stem flow. The influence of air temperature (Ta) during the maturity stage was significantly higher than in other periods. On a daily scale, Rs was the main driving factor of stem flow during the heading stage. During the filling growth stage, the main driving factor of NFP and MP stem flow was RH and Ts, respectively. However, during the maturity stage, the environmental factors had no significant influence on seed-maize stem flow. For different seed-maize plants, the main influential factors were different in each of the three growing seasons. Therefore, we identified them to accurately model the FP and MP stem flow and applied precision irrigation under alternate partial root-zone furrow irrigation to analyze major factors affecting stem flow in different scales.
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QTL Detection and Epistasis Analysis for Heading Date Using Single Segment Substitution Lines in Rice (Oryza sativa L.)
LI Guang-xian, CHEN Ai-hua, LIU Xu, WANG Wen-ying, DING Han-feng, LI Jun, LIU Wei, LI Si-shen , YAO Fang-yin
2014, 13 (
11
): 2311-2321. DOI:
10.1016/S2095-3119(13)60615-2
Abstract
(
1524
)
PDF in ScienceDirect
Heading date of rice is a key agronomic trait determining cultivated areas and seasons and affecting yield. In the present study, five primary single segment substitution lines with the same genetic background were used to detect quantitative trait loci (QTLs) for heading date in rice. Two QTLs, qHD3 and qHD6 on the short arm of chromosome 3 and the short arm of chromosome 6, respectively, were identified under natural long-day (NLD). Nineteen secondary single segment substitution lines (SSSLs) and seven double segments pyramiding lines were designed to map the two QTLs and to evaluate their epistatic interaction between them. By overlapping mapping, qHD3 was mapped in a 791-kb interval between SSR markers RM3894 and RM569 and qHD6 in a 1 125-kb interval between RM587 and RM225. Results revealed the existence of epistatic interaction between qHD3 and qHD6 under natural long-day (NLD). It was also found that qHD3 and qHD6 had significant effects on plant height and yield traits, indicating that both of the QTLs have pleiotropic effects.
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