Search within results Open Search
Please wait a minute...
For Selected: Toggle Thumbnails
Resistance analysis of the rice variety Huaidao 5 against root-knot nematode Meloidogyne graminicola
FENG Hui, ZHOU Can-rong, ZHU Feng, LE Xiu-hu, JING De-dao, Paul DALY, ZHOU Dong-mei, WEI Li-hui
2023, 22 (10): 3081-3089.   DOI: 10.1016/j.jia.2022.11.008
Abstract156)      PDF in ScienceDirect      

Meloidogyne graminicola has emerged as one of the most destructive plant-parasitic nematodes affecting rice (Oryza sativa) production worldwide.  Resistance to Mgraminicola in rice could be the most effective option for its management.  However, sources of germplasm with resistance to Mgraminicola in rice remain limited.  Here, we describe the root attraction, gall formation and genetic analysis of the resistance to Mgraminicola in the rice variety Huidao 5.  A nematode attraction assay showed that second-stage juveniles (J2s) of Mgraminicola were attracted at the root tip of Huaidao 5 within 8 h without a significant reduction in attraction compared to the susceptible rice variety Nanjing 9108.  Microscopic observation of the infection revealed that the J2s invaded root tissues 12 h after inoculation, but their subsequent movement to the root tip was hindered in Huaidao 5, resulting in decreased nematode number compared to Nanjing 9108.  Additionally, we used the soil and hydroponic culture systems to simulate upland and flooding conditions in the paddy fields respectively, and found that gall number was significantly reduced, and nematode development was clearly suppressed in Huaidao 5.  To investigate the genetic basis of this resistance, cross breeding was performed between the Huaidao 5 and Nanjing 9108 varieties.  There was no reduction in the resistance of the F1 offspring to Mgraminicola in the greenhouse or field trials, suggesting that a dominant gene could control resistance in Huaidao 5.  In summary, this study provides a detailed characterization of a novel source of resistance to Mgraminicola in rice, which is of great potential for use in crop breeding.

Reference | Related Articles | Metrics
Effects of dietary amylose to amylopectin ratio on growth performance, carcass quality characteristics and meat fatty acids in Chinese Qinchuan cattle
PIAO Min-yu, HU Feng-ming, KONG Fan-lin, LIU Yun-long, WANG Shuo, CUI Kai, SUN Tao, DIAO Qi-yu, TU Yan
2021, 20 (12): 3256-2169.   DOI: 10.1016/S2095-3119(20)63576-6
Abstract181)      PDF in ScienceDirect      
This study evaluated the effects of the dietary starch amylose/amylopectin ratio on growth performance, rumen fermentation and blood parameters, carcass characteristics and marbling score, and meat fatty acid profiles and transcriptional changes in the genes involved in the gluconeogenesis pathway in Chinese Qinchuan cattle.  Forty-five cattle were randomly divided into three groups.  The bulls were fed a control diet (middle amylose/amylopectin ratio=0.47) or diets with either waxy corn starch (WS) inclusion (low amylose/amylopectin ratio=0.23) or pea starch (PS) inclusion (high amylose/amylopectin ratio=0.60) for 90 days.  The bulls were individually allowed to receive the diets at the daily amount of at least 2.1% of their individual BW twice daily.  The bulls were weighed at 45-day intervals, and blood and rumen fluid samples were also collected at 45-day intervals.  The bulls were slaughtered and longissimus thoracis (LT) samples were collected for meat quality measurements.  Compared with PS, the average daily gain (ADG) and feed efficiency in control and WS were increased (P<0.05) during d 0 to 45.  However, the feed efficiency in PS was increased (P<0.05) compared with the control and WS during d 46 to 90.  During the whole feeding trial, the ADG in control and WS showed trends that were higher (0.05<P<0.1) than that of PS, while other growth performance measures and feed intake did not differ (P>0.05) among the three groups.  Compared with PS, the serum insulin concentration in WS was increased (P<0.05) at d 45, but not at d 90.  Compared with control and WS, the serum SOD and T-AOC concentrations in PS were increased (P<0.05) at d 45, but not at d 90.  Compared with control, the rumen microbial crude protein contents in WS and PS were increased (P<0.05) at d 0, while those of control and WS were higher (P<0.05) than that of PS at d 45, but not at d 90.  Compared with PS, the backfat thickness in control and WS were increased (P<0.05), however, the marbling score and expression of genes related to the gluconeogenesis pathway in liver of the three groups did not differ (P>0.05).  Chemical and physio-chemical compositions of LT did not differ (P>0.05) among the three groups.  In conclusion, diets with low or middle amylose/amylopectin ratios increased the backfat thickness, and tended to improve ADG, but had no effect on other carcass characteristics, fat content in the LT, or the expression of hepatic genes related to the gluconeogenesis pathway in Chinese Qinchuan cattle. 
Keywords: 
Reference | Related Articles | Metrics
Effect of tillage and burial depth and density of seed on viability and seedling emergence of weedy rice
ZHANG Zheng, GAO Ping-lei, DAI Wei-min, SONG Xiao-ling, HU Feng, QIANG Sheng
2019, 18 (8): 1914-1923.   DOI: 10.1016/S2095-3119(19)62583-9
Abstract185)      PDF in ScienceDirect      
Weedy rice (Oryza sativa f. spontanea) is one of the three worst paddy weeds in most rice growing areas.  The unexpected heavy infestation is derived from a persistence of soil seed bank of weedy rice, which the shattered seeds chiefly feed back to.  Information on soil seed bank dynamics is imperative to predict the infestation of weeds.  In the present paper, the effect of rotary tillage on weedy rice seed bank structure was studied first, and a burial experiment of marked seeds was conducted to observe the overwintering survival, seed viability and seedling emergence of weedy rice.  The results showed that the proportion of weedy rice seeds in deeper soil increased but seedling emergence decreased with increasing plowing depth.  The viability of weedy rice seeds decreased as the burial duration time extended but more slowly in deeper soil layers.  Additionally, there was no significant effect of burial density on seed viability.  Moreover, the logistic model fitted well (R2≥0.95, P≤0.01) with the depressive trends of seed viability with increasing burial time under all burial depths and densities which can provide us further information about seed survival.  In field experiments, number of seedling emergence significantly decreased as seed burial depth increased, conversely, proportion of seedling emergence increased as seed burial density decreased.  This study has important implications for determining strategies for weedy rice management by exhausting its seed bank through the alteration of tillage practices.
Reference | Related Articles | Metrics
Hepatitis associated with hepatitis B virus in broilers
ZHAO Yue, MAO Jing-jing, SHE Rui-ping, HU Feng-jiao, Majid H Soomro, LIANG Rui-ping, YANG Yi-fei, DU Fang, WANG Tong-tong, GUO Zhao-jie, CHENG Min-heng
2016, 15 (1): 191-199.   DOI: 10.1016/S2095-3119(14)60914-X
Abstract1647)      PDF in ScienceDirect      
Infection by hepatitis B virus (HBV) results in acute and chronic liver damages in humans. Liver products of broilers as a primary food consumed in our daily life have a close connection with public health. The prevalence of the virus in livers and serum of broilers is of great significance, owning to the potential transmission between chickens and humans. Liver tissues and serum samples were tested to investigate the prevalence of hepatitis B virus infection in slaughtered broilers, for expression of HBV antigens and antibodies. The distribution and positive rate of hepatitis B surface antigen (HBsAg), hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) in liver samples were examined using immunohistochemistry. HBsAg was mainly located in the cytoplasm of hepatocytes with a positivity of 81.61% whereas HBeAg and HBcAg were primarily located in the nucleus of hepatocytes with a positivity of 40.13 and 49.10%, respectively. Enzyme-linked immunosorbent assay (ELISA) analysis of serum for HBV serological markers demonstrated a high prevalence of hepatiits B surface antibody (HBsAb, 54.91%) and hepatitis B core antibody (HBcAb, 27.68%), whereas HBeAb, HBsAg and HBeAg were rarely detectable. Classic hepatitis pathological changes, including swollen hepatocytes, focal parenchymal necrosis, lymphocytic infiltration and hyperplasia of fibrous connective tissues were observed using histopathological analysis. Some of the liver samples were found positive for HBV DNA using nested PCR. Sequence comparison confirmed that all sequences shared 97.5–99.3% identity with human HBV strains. These results demonstrated the existence of HBV in livers and serums of broilers. Animals or animal products contaminated with HBV could raise an important public health concern over food safety and zoonotic risk.
Reference | Related Articles | Metrics
17β-Estradiol Regulates SKP2 Expression in Cultured Immature Boar Sertoli Cells Mainly via Estrogen Receptor β, cAMP-PKA and ERK1/2
WANG Xian-zhong, ZHU Feng-wei, WANG Yong, WANG Yi, ZHANG Jiao-jiao , ZHANG Jia-hua
2014, 13 (4): 827-836.   DOI: 10.1016/S2095-3119(13)60430-X
Abstract1748)      PDF in ScienceDirect      
Estrogen plays an important role in regulating testicular Sertoli cell number. Furthermore, S-phase kinase-associated protein 2 (SKP2) plays a central role in mammalian cell cycle progression. The objective of this study was to determine whether 17β-estradiol can regulate the expression of SKP2, and the Sertoli cell cycle, via estrogen receptor β (ERβ), the cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) and extracellular signal-regulated kinase (ERK1/2) pathway. When cultured immature boar Sertoli cells were treated with 17β-estradiol, a time-dependent increase in SKP2 mRNA and protein level was observed by real-time PCR and Western blot, and 17β-estradiol activity peaked at 30 min. Treatment with ICI182780 and ERβ antagonist reduced 17β-estradiol-induced expression of SKP2 and proliferating cell nuclear antigen (PCNA), while increasing the protein concentration of p27kip1. However, the effect of ERa antagonist on these parameters was lower than that of ICI182780 and ERβ. Forskolin had a similar effect as 17β-estradiol on the expression of SKP2, PCNA and p27kip1. Rp-cAMP, H-89 and U0126 treatment reduced 17β-estradiol-induced changes, while H-89 also inhibited ERK1/2 activation. Therefore, 17β-estradiol mainly regulates SKP2 mRNA and protein expression via ERβ-cAMP-PKA and ERK1/2 activation. SKP2 and PCNA expression were positively correlated, while increased SKP2 expression likely resulted in p27kip1 degradation.
Reference | Related Articles | Metrics