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Mining of candidate genes for grape berry cracking using a genome-wide association study
ZHANG Chuan, WU Jiu-yun, CUI Li-wen, FANG Jing-gui
2022, 21 (8): 2291-2304.   DOI: 10.1016/S2095-3119(21)63881-9
Abstract174)      PDF in ScienceDirect      

Fruit cracking is a common phenomenon during the growth and development of horticultural crops that seriously affects fruit yield and quality.  However, there are few studies on the mining of candidate genes related to berry cracking.  To better understand the genetic basis of grape berry cracking, we conducted a genome-wide association study (GWAS) of grape varieties.  Based on the mixed linear model (MLM), we detected five single nucleotide polymorphism (SNP) loci associated with berry-cracking index and two SNP loci associated with berry-cracking type in two years.  These loci were mainly distributed on four chromosomes, namely 1, 2, 3, and 18, and were associated with ten unique candidate berry-cracking genes.  The gene expression patterns indicated that the candidate genes in the susceptible berry-cracking variety were more abundant than in the resistant berry-cracking variety.  Grape berry-cracking is a complex trait controlled by multiple genes, mainly including genes involved in polygalacturonase, copper transporter, and receptor-like proteins.  The high expression of the candidate berry-cracking genes may promote the occurrence of berry cracking, so the present study helps to further elucidate the genetic mechanism of berry cracking.

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Exploiting push-pull strategy to combat the tea green leafhopper based on volatiles of Lavandula angustifolia and Flemingia macrophylla
HAN Shan-jie, WANG Meng-xin, WANG Yan-su, WANG Yun-gang, CUI Lin, HAN Bao-yu
2020, 19 (1): 193-203.   DOI: 10.1016/S2095-3119(19)62778-4
Abstract181)      PDF in ScienceDirect      
Thirteen volatile compounds were identified from Flemingia macrophylla plants.  Eight major components significantly attracted the tea green leafhoppers, Empoasca flavescens F.  Based on their relative abundances, following synthetic blends were made for field experiments: 1) eight-component-attractant blend included Z-3-hexen-1-ol, Z-3-hexenyl acetate, Z-ocimene, MeSA, Z-3-hexenyl butyrate, dodecane, hexadecane and nonanal at 10, 10, 1, 11, 2, 6, 2 and 4 mg mL–1 in n-hexane, respectively; 2) four-component-attractant blend #1 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and nonanal at 2, 10, 10 and 4 mg mL–1 in n-hexane, respectively; 3) four-component-attractant blend #2 contained hexadecane, Z-3-hexenyl acetate, Z-3-hexen-1-ol and MeSA at 2, 10, 10 and 11 mg mL–1 in n-hexane, respectively.  Thymol and 1-methoxy-4-methyl-2-(1-methylethyl)-benzene, identified from Lavandula angustifolia aeration samples, significantly repelled the leafhopper as strong repellents when tested alone or in combination at 10 mg mL–1.  For field bioassays, each attractant lure was attached to a bud green sticky board hung from a bamboo stick at above tea plant level for catching the leafhoppers, whereas the repellent dispenser was tied to a tea branch inside tea clump for pushing the leafhoppers away from tea clumps.  The results showed that the eight-component-attractant blend caught similar numbers of the leafhopper as did the four-component-attractant blend #1 at about 53–79 leafhoppers/trap/day, which were significantly higher than those on the hexane-control bud green sticky boards.  Average leafhopper catches from un-baited sticky boards were about 51–73 leafhoppers/trap/day when pushed by the repellents placed inside tea plants, with the two-component-repellent blend being more effective than their single components.  When the two-component-repellent blend was further tested with the three attractant blends in a push-pull fashion, average trap catches ranged from 62 to 92 leafhoppers/trap/day.  Control efficacy on the leafhoppers within the push-pull zones increased progressively from day 1 (43%) to day 5 (73%).  This push-pull approach might have a great potential as a green control strategy for combating the tea green leafhoppers. 
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Arbuscular mycorrhizal fungi combined with exogenous calcium improves the growth of peanut (Arachis hypogaea L.) seedlings under continuous cropping
CUI Li, GUO Feng, ZHANG Jia-lei, YANG Sha, MENG Jing-jing, GENG Yun, WANG Quan, LI Xinguo, WAN Shu-bo
2019, 18 (2): 407-416.   DOI: 10.1016/S2095-3119(19)62611-0
Abstract265)      PDF (624KB)(237)      
The growth and yield of peanut are negatively affected by continuous cropping.  Arbuscular mycorrhizal fungi (AMF) and calcium ions (Ca2+) have been used to improve stress resistance in other plants, but little is known about their roles in peanut seedling growth under continuous cropping.  This study investigated the possible roles of the AMF Glomus mosseae combined with exogenous Ca2+ in improving the physiological responses of peanut seedlings under continuous cropping.  G. mosseae combined with exogenous Ca2+ can enhance plant biomass, Ca2+ level, and total chlorophyll content.  Under exogenous Ca2+ application, the Fv/Fm in arbuscular mycorrhizal (AM) plant leaves was higher than that in the control plants when they were exposed to high irradiance levels.  The peroxidase, superoxide dismutase, and catalase activities in AM plant leaves also reached their maximums, and accordingly, the malondialdehyde content was the lowest compared to other treatments.  Additionally, root activity, and content of total phenolics and flavonoids were significantly increased in AM plant roots treated by Ca2+ compared to either G. mosseae inoculation or Ca2+ treatment alone.  Transcription levels of AhCaM, AhCDPK, AhRAM1, and AhRAM2 were significantly improved in AM plant roots under exogenous Ca2+ treatment.  This implied that exogenous Ca2+ might be involved in the regulation of G. mosseae colonization of peanut plants, and in turn, AM symbiosis might activate the Ca2+ signal transduction pathway.  The combination of AMF and Ca2+ benefitted plant growth and development under continuous cropping, suggesting that it is a promising method to cope with the stress caused by continuous cropping.
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Effects of different drying methods on quality, bacterial viability and storage stability of probiotic enriched apple snacks
CUI Li, NIU Li-ying, LI Da-jing, LIU Chun-quan, LIU Ying-ping, LIU Chun-ju, SONG Jiang-feng
2018, 17 (01): 247-255.   DOI: 10.1016/S2095-3119(17)61742-8
Abstract688)      PDF in ScienceDirect      
Effects of four different drying methods on the colour, texture, sensory quality, microstructure, bacterial viability and storage stability of probiotic-enriched apple snacks were assessed.  The drying methods were air drying (AD), freeze drying (FD), freeze drying followed by microwave vacuum drying (FD+MVD) and air drying followed by explosion puffing drying (AD+EPD).  Overall, FD+MVD can be used as a suitable drying method for the development of probiotic enriched apple snacks in consideration of colour, texture, sensory quality, bacterial viability and storage stability.  Probiotic bacteria in FD+MVD-dried samples remained above 1×106 CFU g–1 for 120 days at 25°C.  Interestingly, bacterial viability in FD+MVD-dried samples turned out to be significantly higher than FD-dried samples during storage for 120 days.  
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Isolation and expression analysis of NtCHS6, a new chalcone synthase gene from Nicotiana tabacum
CHEN Shuai, ZHANG Yin-chao, PAN Xu-hao, LI Yi-ting, CUI Li-jie, WU Feng-yan, CAO Mo-ju, YANG Ai-guo , PAN Guang-tang
2017, 16 (07): 1443-1450.   DOI: 10.1016/S2095-3119(16)61503-4
Abstract771)      PDF in ScienceDirect      
Chalcone synthases (CHS, EC 2.3.1.74) are key enzymes that catalyze the first committed step in flavonoid biosynthesis. In this study, we isolated a chalcone synthase, named NtCHS6, from Nicotiana tabacum.  This synthase shared high homology with the NSCHSL (Y14507) gene and contained most of the conserved active sites that are in CHS proteins.  The phylogenetic analysis suggested that NtCHS6 protein shared a large genetic distance with other Solanaceae CHS proteins and was the most closely-related to an uncharacterized CHS from Solanum lycopersicum.  The expression analysis indicated
that NtCHS6 was abundantly expressed in leaves, especially in mature leaves.  By scrutinizing its upstream promoter sequences, multiple cis-regulatory elements involved in light and drought responsive were detected.  Furthermore, NtCHS6 expression decreased significantly under dark treatment and increased significantly under drought stress.  Our results suggested that NtCHS6 expression exhibited both light responsiveness and drought responsiveness, and might play important roles in ultraviolet protection and drought tolerance.
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Prediction Model of Weekly Retail Price for Eggs Based on Chaotic Neural Network
LI Zhe-min, CUI Li-guo, XU Shi-wei, WENG Ling-yun, DONG Xiao-xia, LI Gan-qiong , YU Hai-peng
2013, 12 (12): 2292-2299.   DOI: 10.1016/S2095-3119(13)60610-3
Abstract1424)      PDF in ScienceDirect      
This paper establishes a short-term prediction model of weekly retail prices for eggs based on chaotic neural network with the weekly retail prices of eggs from January 2008 to December 2012 in China. In the process of determining the structure of the chaotic neural network, the number of input layer nodes of the network is calculated by reconstructing phase space and computing its saturated embedding dimension, and then the number of hidden layer nodes is estimated by trial and error. Finally, this model is applied to predict the retail prices of eggs and compared with ARIMA. The result shows that the chaotic neural network has better nonlinear fitting ability and higher precision in the prediction of weekly retail price of eggs. The empirical result also shows that the chaotic neural network can be widely used in the field of short-term prediction of agricultural prices.
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Price Transmission in China’s Swine Industry with an Application of MCM
XU Shi-wei, LI Zhe-min, CUI Li-guo, DONG Xiao-xia, KONG Fan-tao, LI Gan-qiong
2012, 12 (12): 2097-2106.   DOI: 10.1016/S1671-2927(00)8748
Abstract1210)      PDF in ScienceDirect      
The paper studies the relationship, adjustment ability, path, efficiency and intensity of price transmission in the swine industry chain in China, which consists of the prices of corn, compound feed for fattening pig, piglet, pig and pork. Monthly prices covering a period of 18 yr (1994-2011) are analyzed using a Market-Chain Cooperated Model (MCM). The empirical results show that there exists a stable long-term cointegration and short-term dynamic relationship in the price system. First, the adjustment speed of each price series is very slow and the transmission path is top-down and one-way significantly. Second, the price from upstream to downstream lags about 2 mon, while there is no lag in price transmission from midstream to downstream. Third, in terms of price transmission intensity, the price of pig impacted greatly on pork price, not only in the current period but also through the whole period. Besides, the price of corn has the largest lagged effects on pork price. According to the above empirical results, we suggest that government should strengthen monitoring and early warning of the swine industry chain, especially the upstream and midstream, attach great importance to the timely adjustment of feed prices and perfect the measures of price subsidy.
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