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Prediction of the potential distribution and analysis of the freezing injury risk of winter wheat on the Loess Plateau under climate change
Qing Liang, Xujing Yang, Yuheng Huang, Zhenwei Yang, Meichen Feng, Mingxing Qing, Chao Wang, Wude Yang, Zhigang Wang, Meijun Zhang, Lujie Xiao, Xiaoyan Song
2024, 23 (9): 2941-2954.   DOI: 10.1016/j.jia.2024.02.006
Abstract81)      PDF in ScienceDirect      
Determining the suitable areas for winter wheat under climate change and assessing the risk of freezing injury are crucial for the cultivation of winter wheat.  We used an optimized Maximum Entropy (MaxEnt) Model to predict the potential distribution of winter wheat in the current period (1970–2020) and the future period (2021–2100) under four shared socioeconomic pathway scenarios (SSPs).  We applied statistical downscaling methods to downscale future climate data, established a scientific and practical freezing injury index (FII) by considering the growth period of winter wheat, and analyzed the characteristics of abrupt changes in winter wheat freezing injury by using the Mann-Kendall (M-K) test.  The results showed that the prediction accuracy AUC value of the MaxEnt Model reached 0.976.  The minimum temperature in the coldest month, precipitation in the wettest season and annual precipitation were the main factors affecting the spatial distribution of winter wheat.  The total suitable area of winter wheat was approximately 4.40×107 ha in the current period.  In the 2070s, the moderately suitable areas had the greatest increase by 9.02×105 ha under SSP245 and the least increase by 6.53×105 ha under SSP370.  The centroid coordinates of the total suitable areas tended to move northward.  The potential risks of freezing injury in the high-latitude and -altitude areas of the Loess Plateau, China increased significantly.  The northern areas of Xinzhou in Shanxi Province, China suffered the most serious freezing injury, and the southern areas of the Loess Plateau suffered the least.  Environmental factors such as temperature, precipitation and geographical location had important impacts on the suitable area distribution and freezing injury risk of winter wheat.  In the future, greater attention should be paid to the northward boundaries of both the winter wheat planting areas and the areas of freezing injury risk to provide the early warning of freezing injury and implement corresponding management strategies.


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BnaSD.C3 is a novel major quantitative trait locus affecting semi-dwarf architecture in Brassica napus L.
WANG Xiao-dong, CAI Ying, PANG Cheng-ke, ZHAO Xiao-zhen, SHI Rui, LIU Hong-fang, CHEN Feng, ZHANG Wei, FU San-xiong, HU Mao-long, HUA Wei, ZHENG Ming, ZHANG Jie-fu
2023, 22 (10): 2981-2992.   DOI: 10.1016/j.jia.2023.02.017
Abstract217)      PDF in ScienceDirect      

Plant height is a key plant architectural trait that affects the seed yield, harvest index and lodging resistance in Brassica napus L., although the genetic mechanisms affecting plant height remain unclear.  Here, a semi-dwarf mutant, df34, was obtained by ethyl methanesulphonate-induced mutagenesis.  Genetic analysis showed that the semi-dwarf phenotype is controlled by one semi-dominant gene, which was located on chromosome C03 using a bulked segregant analysis coupled with whole-genome sequencing, and this gene was named BnaSD.C3.  Then BnaSD.C3 was fine-mapped to a 297.35-kb segment of the “Darmor-bzh” genome, but there was no potential candidate gene for the semi-dwarf trait underlying this interval.  Furthermore, the interval was aligned to the Zhongshuang 11 reference genome.  Finally, combining structural variation analysis, transcriptome sequencing, phytohormone analyses and gene annotation information, BnaC03G0466900ZS and BnaC03G0478900ZS were determined to be the most likely candidate genes affecting the plant height of df34.  This study provides a novel major locus for breeding and new insights into the genetic architecture of plant height in Bnapus

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Mechanization and efficiency in rice production in China
SHI Min, Krishna P. PAUDEL, CHEN Feng-bo
2021, 20 (7): 1996-2008.   DOI: 10.1016/S2095-3119(20)63439-6
Abstract188)      PDF in ScienceDirect      
Agricultural mechanization and custom machine services have developed rapidly in China, which can influence rice production efficiency in the future.  We calculate technical efficiency, allocative efficiency, and scale efficiency using data collected in 2015 from a face-to-face interview survey of 450 households that cultivated 3 096 plots located in the five major rice-producing provinces of China.  We use a one-step stochastic frontier model to calculate technical efficiency and regress the efficiency scores on socio-demographic and physical land characteristics to find the influencing variables.  Variables influencing technical efficiency are compared at three different phases of rice cultivation.  We also calculate technical efficiency by using the Heckman Selection Model, which addresses technological heterogeneity and self-selection bias.  Results indicate that: (1) the average value of technical efficiency using a one-step stochastic frontier model was found to be 0.74.  When self-selection bias is accounted for using the Heckman Selection Model, the average value of the technical efficiency increases to 0.80; (2) mechanization at the chemical application phase has a positive effect on technical efficiency, but mechanization does not affect efficiency at the plowing and harvesting phases; (3) machines are overused relative to both land and labor, and high machine input use on the small size of landholding has resulted in allocative inefficiency; (4) rice farmers are overwhelmingly operating at a sub-optimal scale.  Future policies should focus on encouraging farmland transfer in rural areas to achieve scale efficiency and allocative efficiency while promoting mechanization at the chemical application phase of rice cultivation to improve technical efficiency. 
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Carbendazim sensitivity in populations of Colletotrichum gloeosporioides complex infecting strawberry and yams in Hubei Province of China
HAN Yong-chao, ZENG Xiang-guo, XIANG Fa-yun, ZHANG Qing-hua, GUO Cong, CHEN Feng-ying, GU Yu-chen
2018, 17 (06): 1391-1400.   DOI: 10.1016/S2095-3119(17)61854-9
Abstract539)      PDF in ScienceDirect      
The ascomycete fungus Colletotrichum gloeosporioides is a devastating plant pathogen with a wide host range and worldwide distribution.  Carbendazim has been widely used to control anthracnose caused by the C. gloeosporioides complex in China for more than 30 years and resistance to carbendazim has been reported in China.  A total of 125 Colletotrichum isolates of strawberry and yam were collected from different geographical regions in Hubei Province, China.  Approximately 52.8% of Colletotrichum spp. isolates showed resistance to carbendazim.  The isolates tested in this study belong to four species, and the frequencies of resistant isolates differed across Colletotrichum species.  Resistant isolates were found in C. siamense and C. fructicola.  In contrast, all isolates of C. gloeosporioides and C. aenigma were sensitive to carbendazim.  Highly carbendazim-resistant isolates harbored the E198A mutation in the β-tubulin 2 (TUB2) gene, whereas moderately carbendazim-resistant isolates harbored the F200Y mutation in the TUB2 gene.  Carbendazim-sensitive Colletotrichum isolates in this study were not genetically similar enough to form a separate cluster from resistant isolates.  The result of this study emphasizes the importance of knowing which Colletotrichum sp. is present, when strategies for disease control are made.
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Inheritance and molecular characterization of resistance to AHAS-inhibiting herbicides in rapeseed
HU Mao-long, PU Hui-ming, GAO Jian-qin, LONG Wei-hua, CHEN Feng, ZHOU Xiao-ying, ZHANG Wei, PENG Qi, CHEN Song, ZHANG Jie-fu
2017, 16 (11): 2421-2433.   DOI: 10.1016/S2095-3119(17)61659-9
Abstract791)      PDF in ScienceDirect      
Rapeseed is a very important oil crop in China; however, its production is challenging due to the absence of effective weed management strategies.  This is predominantly because of a shortage of herbicide resistance genes.  Acetohydroxyacid synthase (AHAS) herbicides inhibit AHAS, a key enzyme involved in branched-chain amino acid synthesis that is required for plant growth.  A rapeseed line designated M342 with AHAS herbicide resistance was developed through seed mutagenesis and was studied to assess the level and mode of inheritance of the resistance and to identify the molecular basis of resistance.  M342 possessed a high level of cross-resistance to sulfonylureas (SUs) and imidazolinones (IMIs).  This resistance was due to AHAS insensitivity to these herbicides and was inherited as a dominant trait conferred by a single nuclear-encoded gene.  Molecular analysis revealed the presence of a Trp574Leu mutation in M342, and an allele-specific cleaved amplified polymorphic sequence (AS-CAPS) marker was developed and cosegregated with herbicide resistance in the F2, BC1, and BC2 populations.  This mutation altered the transcript levels of BnAHAS1 and BnAHAS3 in M342 compared with those in the wild type, but it did not affect the agronomic or quality traits.  The simple genetic inheritance of this mutation and the availability of the cleaved amplified polymorphic sequence (CAPS) marker and herbicide resistance gene should facilitate the development of herbicide-resistant rapeseed cultivars for effective weed control in China.  
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Sequence and phylogenetic analysis of hemagglutinin genes of H9N2 influenza viruses isolated from chicken in China from 2013 to 2015
SU Xiao-na, XIE Qing-mei, LIAO Chang-tao, YAN Zhuan-qiang, CHEN Wei-guo, BI Ying-zuo, CHEN Feng
2016, 15 (11): 2604-2612.   DOI: 10.1016/S2095-3119(16)61406-5
Abstract1285)      PDF in ScienceDirect      
    H9N2 avian influenza virus (AIV) infection is a major problem in poultry industry worldwide. In this study, molecular characterizations and phylogenetic relationships of hemagglutinin (HA) gene sequences of H9N2 AIV of 5 Chinese isolates in 2014 recently available in GenBank, 3 widely used vaccine strains, and 52 novel isolates in China from 2013 to 2015 were analyzed. The homology analysis showed that the nucleotide sequences of HA gene of these recent Chinese H9N2 AIV isolates shared homologies from 94.1 to 99.9%. Phylogenetic analysis showed that all isolates belonged to AIV lineage h9.4.2.5. Fifty-six out of the 57 recent Chinese H9N2 AIV isolates had the motifs PSRSSR↓GLF at the cleavage sites within the HA protein, while one isolate PWH01 harbored LSRSSR↓GLF. Remarkably, all of the recent Chinese H9N2 AIV strains had the Q216L substitution in the receptor binding site, which indicated that they had potential to infect humans. Most of recent Chinese H9N2 AIV isolates lost the potential N-linked glycosylation site at residues 200–202 compared with vaccine strains. This present study demonstrated that AIV lineage h9.4.2.5 was more predominant in China than other lineages as it harbored all the H9N2 AIV isolated between 2013 and 2015. Also we showed the importance of continuous surveillance of emerging H9N2 AIV in China and update of vaccine formulation accordingly in order to prevent and control H9N2 AIV.
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