Expression Pattern Analysis of Zinc Finger Protein Genes in Wheat (Triticum aestivum L.) Under Phosphorus Deprivation

doi:10.1016/S2095-3119(13)60739-X

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (8): 1621-1633.DOI: 10.1016/S2095-3119(13)60739-X

Expression Pattern Analysis of Zinc Finger Protein Genes in Wheat (Triticum aestivum L.) Under Phosphorus Deprivation

LIXiao-juan2, GUOCheng-jin1, LUWen-jing2, DUANWei-wei1, ZHAOMiao3, MAChun-ying1, GUJun-tao2, XIAOKai1

1、College of Agronomy, Agricultural University of Hebei, Baoding 071001, P.R.China
2、College of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.China
3、Science & Technology College, North China Electric Power University, Baoding 071051, P.R.China

收稿日期:2013-10-21 出版日期:2014-08-01 发布日期:2014-08-02
通讯作者: XIAO Kai, Tel: +86-312-7528115, Fax: +86-312-7528400, E-mail: xiaokai@hebau.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (31201674 and 31371618), the Natural Science Foundation of Hebei Province, China (C2011204031) and the Key Laboratory of Crop Growth Regulation of Hebei Province, China.

Expression Pattern Analysis of Zinc Finger Protein Genes in Wheat (Triticum aestivum L.) Under Phosphorus Deprivation

LIXiao-juan2, GUOCheng-jin1, LUWen-jing2, DUANWei-wei1, ZHAOMiao3, MAChun-ying1, GUJun-tao2, XIAOKai1

1、College of Agronomy, Agricultural University of Hebei, Baoding 071001, P.R.China
2、College of Life Sciences, Agricultural University of Hebei, Baoding 071001, P.R.China
3、Science & Technology College, North China Electric Power University, Baoding 071051, P.R.China

Received:2013-10-21 Online:2014-08-01 Published:2014-08-02
Contact: XIAO Kai, Tel: +86-312-7528115, Fax: +86-312-7528400, E-mail: xiaokai@hebau.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (31201674 and 31371618), the Natural Science Foundation of Hebei Province, China (C2011204031) and the Key Laboratory of Crop Growth Regulation of Hebei Province, China.

摘要/Abstract

摘要： Zinc finger protein (ZFP) genes comprise a large and diverse gene family, and are involved in biotic and abiotic stress responses in plants. In this study, a total of 126 ZFP genes classified into various types in wheat were characterized and subjected to expression pattern analysis under inorganic phosphate (Pi) deprivation. The wheat ZFP genes and their corresponding GenBank numbers were obtained from the information of a 4×44K wheat gene expression microarray chip. They were confirmed by sequence similarity analysis and named based on their homologs in Brachypodium distachyon or Oriza sativa. Expression analysis based on the microarray chip revealed that these ZFP genes are categorized into 11 classes according to their gene expression patterns in a 24-h of Pi deprivation regime. Among them, ten genes were differentially up-regulated, ten genes differentially down- regulated, and two genes both differentially up- and down-regulated by Pi deprivation. The differentially up- or down-regulated genes exhibited significantly more or less transcripts at one, two, or all of the checking time points (1, 6, and 24 h) of Pi stress in comparison with those of normal growth, respectively. The both differentially up- and down-regulated genes exhibited contrasting expression patterns, of these, TaWRKY70;5 showed significantly up-regulated at 1 and 6 h and down-regulated at 24 h whereas TaAN1AN20-8;2 displayed significantly upregulated at 1 h and downregulated at 6 h under deprivation Pi condition. Real time PCR analysis confirmed the expression patterns of the differentially expressed genes obtained by the microarray chip. Our results indicate that numerous ZFP genes in wheat respond to Pi deprivation and have provided further insight into the molecular basis that plants respond to Pi deprivation mediated by the ZFP gene family.

关键词: wheat (Triticum aestivum L.) , zinc finger protein , Pi deprivation , gene expression

Abstract: Zinc finger protein (ZFP) genes comprise a large and diverse gene family, and are involved in biotic and abiotic stress responses in plants. In this study, a total of 126 ZFP genes classified into various types in wheat were characterized and subjected to expression pattern analysis under inorganic phosphate (Pi) deprivation. The wheat ZFP genes and their corresponding GenBank numbers were obtained from the information of a 4×44K wheat gene expression microarray chip. They were confirmed by sequence similarity analysis and named based on their homologs in Brachypodium distachyon or Oriza sativa. Expression analysis based on the microarray chip revealed that these ZFP genes are categorized into 11 classes according to their gene expression patterns in a 24-h of Pi deprivation regime. Among them, ten genes were differentially up-regulated, ten genes differentially down- regulated, and two genes both differentially up- and down-regulated by Pi deprivation. The differentially up- or down-regulated genes exhibited significantly more or less transcripts at one, two, or all of the checking time points (1, 6, and 24 h) of Pi stress in comparison with those of normal growth, respectively. The both differentially up- and down-regulated genes exhibited contrasting expression patterns, of these, TaWRKY70;5 showed significantly up-regulated at 1 and 6 h and down-regulated at 24 h whereas TaAN1AN20-8;2 displayed significantly upregulated at 1 h and downregulated at 6 h under deprivation Pi condition. Real time PCR analysis confirmed the expression patterns of the differentially expressed genes obtained by the microarray chip. Our results indicate that numerous ZFP genes in wheat respond to Pi deprivation and have provided further insight into the molecular basis that plants respond to Pi deprivation mediated by the ZFP gene family.

Key words: wheat (Triticum aestivum L.) , zinc finger protein , Pi deprivation , gene expression

LIXiao-juan2, GUOCheng-jin1, LUWen-jing2, DUANWei-wei1, ZHAOMiao3, MAChun-ying1, GUJun-tao2, XIAOKai1. Expression Pattern Analysis of Zinc Finger Protein Genes in Wheat (Triticum aestivum L.) Under Phosphorus Deprivation[J]. Journal of Integrative Agriculture, 2014, 13(8): 1621-1633.

LIXiao-juan2 , GUOCheng-jin1 , LUWen-jing2 , DUANWei-wei1 , ZHAOMiao3 , MAChun-ying1 , GUJun-tao2 , XIAOKai1 . Expression Pattern Analysis of Zinc Finger Protein Genes in Wheat (Triticum aestivum L.) Under Phosphorus Deprivation[J]. Journal of Integrative Agriculture, 2014, 13(8): 1621-1633.

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Expression Pattern Analysis of Zinc Finger Protein Genes in Wheat (Triticum aestivum L.) Under Phosphorus Deprivation

Expression Pattern Analysis of Zinc Finger Protein Genes in Wheat (Triticum aestivum L.) Under Phosphorus Deprivation

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