Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (3): 493-501.DOI: 10.1016/S2095-3119(15)61050-4

• 论文 •    下一篇

A genetic diversity assessment of starch quality traits in rice landraces from the Taihu basin, China

 AO Yan, XU Yong, CUI Xiao-fen, WANG An, TENG Fei, SHEN Li-qun, LIU Qiao-quan   

  1. 1、Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Co-Innovation Center for Modern Production Technology of
    Grain Crops/College of Agriculture, Yangzhou University, Yangzhou 225009, P.R.China
    2、Suzhou Chien-Shiung Institute of Technology, Suzhou 215411, P.R.China
  • 收稿日期:2015-02-06 出版日期:2016-03-07 发布日期:2016-03-09
  • 通讯作者: LIU Qiao-quan, Tel: +86-514-87979242,E-mail: qqliu@yzu.edu.cn
  • 作者简介:AO Yan, Mobile: +86-18051236202, E-mail: 350685056@qq.com;
  • 基金资助:

    This work was financially supported by the National Natural Science Foundation of China (30800603), the Science and Technology Plan Projects of Taicang City, China (TC214YY3) and the Building Program of the Science and Technology Innovation Team of Chien-Shiung Institute of Technology, China (2013CX02).

A genetic diversity assessment of starch quality traits in rice landraces from the Taihu basin, China

 AO Yan, XU Yong, CUI Xiao-fen, WANG An, TENG Fei, SHEN Li-qun, LIU Qiao-quan   

  1. 1、Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Co-Innovation Center for Modern Production Technology of
    Grain Crops/College of Agriculture, Yangzhou University, Yangzhou 225009, P.R.China
    2、Suzhou Chien-Shiung Institute of Technology, Suzhou 215411, P.R.China
  • Received:2015-02-06 Online:2016-03-07 Published:2016-03-09
  • Contact: LIU Qiao-quan, Tel: +86-514-87979242,E-mail: qqliu@yzu.edu.cn
  • About author:AO Yan, Mobile: +86-18051236202, E-mail: 350685056@qq.com;
  • Supported by:

    This work was financially supported by the National Natural Science Foundation of China (30800603), the Science and Technology Plan Projects of Taicang City, China (TC214YY3) and the Building Program of the Science and Technology Innovation Team of Chien-Shiung Institute of Technology, China (2013CX02).

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摘要： There are nearly 1 000 rice landrace varieties in the Taihu basin, China. To assess the genetic diversity of the rice, 24 intragenic molecular markers (representing 17 starch synthesis-related genes) were investigated in 115 Taihu basin rice landraces and 87 improved cultivars simultaneously. The results show that the average genetic diversity and polymorphism information content values of the landraces were higher than those of improved cultivars. In total, 41 and 39 allele combinations (of the 17 genes) were derived from the landraces and improved cultivars, respectively; only two identical allele combinations were found between the two rice variety sources. Cluster analysis, based on the molecular markers, revealed that the rice varieties could be subdivided into five groups and, within these, the japonica improved rice and japonica landrace rice varieties were in two separate groups. According to the quality reference criteria to classify the rice into grades, some of the landraces were found to perform well, in terms of starch quality. For example, according to NY/T595-2002 criteria from the Ministry of Agriculture of China, 25 and 33 landraces reached grade 1, in terms of their apparent amylose content and gel consistency. The varieties that had outstanding quality could be used as breeding materials for rice quality breeding programs in the future. Our study is useful for future applications, such as genetic diversity studies, the protection of rice variety and improvment of rice quality in breeding programs.

关键词: intragenic molecular marker , starch synthesis , improved cultivars , cluster analysis , polymorphism information content

Abstract: There are nearly 1 000 rice landrace varieties in the Taihu basin, China. To assess the genetic diversity of the rice, 24 intragenic molecular markers (representing 17 starch synthesis-related genes) were investigated in 115 Taihu basin rice landraces and 87 improved cultivars simultaneously. The results show that the average genetic diversity and polymorphism information content values of the landraces were higher than those of improved cultivars. In total, 41 and 39 allele combinations (of the 17 genes) were derived from the landraces and improved cultivars, respectively; only two identical allele combinations were found between the two rice variety sources. Cluster analysis, based on the molecular markers, revealed that the rice varieties could be subdivided into five groups and, within these, the japonica improved rice and japonica landrace rice varieties were in two separate groups. According to the quality reference criteria to classify the rice into grades, some of the landraces were found to perform well, in terms of starch quality. For example, according to NY/T595-2002 criteria from the Ministry of Agriculture of China, 25 and 33 landraces reached grade 1, in terms of their apparent amylose content and gel consistency. The varieties that had outstanding quality could be used as breeding materials for rice quality breeding programs in the future. Our study is useful for future applications, such as genetic diversity studies, the protection of rice variety and improvment of rice quality in breeding programs.

Key words: intragenic molecular marker , starch synthesis , improved cultivars , cluster analysis , polymorphism information content