Response of Cytochrome P450 Expression to Maize Volatiles in Helicoverpa armigera (Hübner)

doi:10.1016/S2095-3119(13)60282-8

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (4): 646-652.DOI: 10.1016/S2095-3119(13)60282-8

Response of Cytochrome P450 Expression to Maize Volatiles in Helicoverpa armigera (Hübner)

WU Shao-ying, SHI Xue-yan, WANG Yi , GAO Xi-wu

1.Department of Entomology, China Agricultural University, Beijing 100193, P.R.China
2.College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, P.R.China

收稿日期:2012-04-25 出版日期:2013-04-01 发布日期:2013-04-07
通讯作者: Correspondence GAO Xi-wu, E-mail: gaoxiwu@263.net.cn
基金资助:
This work was supported by the National Basic Research Programme of China (2012CB114103), and the National Natural Science Foundation of China (31171887, 31201541).

Response of Cytochrome P450 Expression to Maize Volatiles in Helicoverpa armigera (Hübner)

WU Shao-ying, SHI Xue-yan, WANG Yi , GAO Xi-wu

1.Department of Entomology, China Agricultural University, Beijing 100193, P.R.China
2.College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, P.R.China

Received:2012-04-25 Online:2013-04-01 Published:2013-04-07
Contact: Correspondence GAO Xi-wu, E-mail: gaoxiwu@263.net.cn
Supported by:
This work was supported by the National Basic Research Programme of China (2012CB114103), and the National Natural Science Foundation of China (31171887, 31201541).

摘要/Abstract

摘要： The 7-ethoxycoumarin O-deethylase (ECOD) activities of cytochrome P450s and differential expression of six cytochrome P450 genes induced by the volatiles from both damaged and undamaged maize plants were investigated in the cotton bollworm, Helicoverpa armigera (Hübner). The ECOD activity changed with time of exposure to maize volatiles. At 36 h after cotton bollworm larvae exposure to maize volatiles, the ECOD activities in cotton bollworm damaged and artificially damaged groups were 2.36 and 4.53 times higher than the control group respectively. The relative expression levels of CYP4S1, CYP6B2 and CYP6B7 in the cotton bollworm were significantly increased in artificially damaged plant group, which was 2.93, 5.09 and 10.66 times higher than that in the control group, respectively. The expression levels of CYP6B2, CYP6B6, CYP9A12, and CYP9A14 were much lower in the larvae exposure to volatiles from both healthy and pest damaged maize seedlings than in the control group at 12 h after larvae exposure to maize volatiles. For the cotton bollworm damaged maize group, the expression of CYP4S1 and CYP9A14 increased.

关键词: 7-ethoxycoumarin O-deethylase , cytochrome P450 gene , maize volatile , Helicoverpa armigera

Abstract: The 7-ethoxycoumarin O-deethylase (ECOD) activities of cytochrome P450s and differential expression of six cytochrome P450 genes induced by the volatiles from both damaged and undamaged maize plants were investigated in the cotton bollworm, Helicoverpa armigera (Hübner). The ECOD activity changed with time of exposure to maize volatiles. At 36 h after cotton bollworm larvae exposure to maize volatiles, the ECOD activities in cotton bollworm damaged and artificially damaged groups were 2.36 and 4.53 times higher than the control group respectively. The relative expression levels of CYP4S1, CYP6B2 and CYP6B7 in the cotton bollworm were significantly increased in artificially damaged plant group, which was 2.93, 5.09 and 10.66 times higher than that in the control group, respectively. The expression levels of CYP6B2, CYP6B6, CYP9A12, and CYP9A14 were much lower in the larvae exposure to volatiles from both healthy and pest damaged maize seedlings than in the control group at 12 h after larvae exposure to maize volatiles. For the cotton bollworm damaged maize group, the expression of CYP4S1 and CYP9A14 increased.

Key words: 7-ethoxycoumarin O-deethylase , cytochrome P450 gene , maize volatile , Helicoverpa armigera

WU Shao-ying, SHI Xue-yan, WANG Yi , GAO Xi-wu. Response of Cytochrome P450 Expression to Maize Volatiles in Helicoverpa armigera (Hübner)[J]. Journal of Integrative Agriculture, 2013, 12(4): 646-652.

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Response of Cytochrome P450 Expression to Maize Volatiles in Helicoverpa armigera (Hübner)

Response of Cytochrome P450 Expression to Maize Volatiles in Helicoverpa armigera (Hübner)

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