Cytological Behavior of Hybridization Barriers Between Oryza sativa and Oryza officinalis

doi:10.1016/S1671-2927(11)60143-0

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (10): 1489-1500.DOI: 10.1016/S1671-2927(11)60143-0

• 论文 • 下一篇

Cytological Behavior of Hybridization Barriers Between Oryza sativa and Oryza officinalis

FU Xue-lin, LU Yong-gen, LIU Xiang-dong, LI Jin-quan , ZHAO Xing-juan

1.Key Laboratory of Plant Molecular Breeding, Guangdong Province/College of Agriculture, South China Agricultural University

收稿日期:2010-08-21 出版日期:2011-10-01 发布日期:2011-10-18
通讯作者: Correspondence LU Yong-gen, Professor, E-mail: yglu@scau.edu.cn
作者简介:FU Xue-lin, Associate Professor, Ph D, E-mail: fuxuelin@scau.edu.cn
基金资助:
This study was financially supported by the Key Project of the Natural Science Foundation of Guangdong Province, China (021037), the Natural Science Foundation of Guangdong Province, China (9151064201000067), the Special Fund for Agro-Scientific Research in the Public Interest, China (201003021), and the Joint Fund of the National Natural Science Foundation of China- Guangdong Province (U0631003).

Cytological Behavior of Hybridization Barriers Between Oryza sativa and Oryza officinalis

FU Xue-lin, LU Yong-gen, LIU Xiang-dong, LI Jin-quan , ZHAO Xing-juan

1.Key Laboratory of Plant Molecular Breeding, Guangdong Province/College of Agriculture, South China Agricultural University

Received:2010-08-21 Online:2011-10-01 Published:2011-10-18
Contact: Correspondence LU Yong-gen, Professor, E-mail: yglu@scau.edu.cn
About author:FU Xue-lin, Associate Professor, Ph D, E-mail: fuxuelin@scau.edu.cn
Supported by:
This study was financially supported by the Key Project of the Natural Science Foundation of Guangdong Province, China (021037), the Natural Science Foundation of Guangdong Province, China (9151064201000067), the Special Fund for Agro-Scientific Research in the Public Interest, China (201003021), and the Joint Fund of the National Natural Science Foundation of China- Guangdong Province (U0631003).

摘要/Abstract

摘要： Oryza officinalis is one of the important wild species in the tertiary gene pool of Oryza sativa. It has a number of elite genes for rice breeding in resistance or tolerance. However, breeding barriers are so serious that the gene transfer is much difficult by sexual cross method between O. sativa and O. officinalis. Characteristics of the breeding barriers were systemically studied in this paper. When both the diploid (AA, 2n=2x=24) and autotetraploid (AAAA, 2n=4x=48) cultivated rice were crossed as maternal parents with O. officinalis (CC, 2n=2x=24), none F1 hybrid seeds were obtained. The young hybrid ovaries aborted at 13-16 d after pollinations (DAP). By rescuing hybrid embryos, in vitro F1 plantlets were obtained in 2x×2x combinations with the crossabilities lower than 0.5%. Lower rates of double-fertilization and abnormal development of hybrid embryo and endosperm were mainly observed in both combinations of 2x×2x and 4x×2x. Free endosperm nuclei in hybrid degenerated early at 1 DAP in a large scale. Almost no normal endosperm cells formed at 3 DAP. Development of a lot of embryos ceased at globular- or pear-shaped stage as well as some degenerated gradually. The hybrid plantlets were both male and female sterility. Due to the abnormal development, a diversity of abnormal embryo sacs formed in hybrids, and hybrid pollen grains were typically abortive. It showed that conflicts of genome A and C in hybrid induced abnormal meioses of meiocytes.

关键词: breeding barriers, interspecific hybridization, crossability, hybrid sterility, Oryza sativa, Oryza officinalis

Abstract: Oryza officinalis is one of the important wild species in the tertiary gene pool of Oryza sativa. It has a number of elite genes for rice breeding in resistance or tolerance. However, breeding barriers are so serious that the gene transfer is much difficult by sexual cross method between O. sativa and O. officinalis. Characteristics of the breeding barriers were systemically studied in this paper. When both the diploid (AA, 2n=2x=24) and autotetraploid (AAAA, 2n=4x=48) cultivated rice were crossed as maternal parents with O. officinalis (CC, 2n=2x=24), none F1 hybrid seeds were obtained. The young hybrid ovaries aborted at 13-16 d after pollinations (DAP). By rescuing hybrid embryos, in vitro F1 plantlets were obtained in 2x×2x combinations with the crossabilities lower than 0.5%. Lower rates of double-fertilization and abnormal development of hybrid embryo and endosperm were mainly observed in both combinations of 2x×2x and 4x×2x. Free endosperm nuclei in hybrid degenerated early at 1 DAP in a large scale. Almost no normal endosperm cells formed at 3 DAP. Development of a lot of embryos ceased at globular- or pear-shaped stage as well as some degenerated gradually. The hybrid plantlets were both male and female sterility. Due to the abnormal development, a diversity of abnormal embryo sacs formed in hybrids, and hybrid pollen grains were typically abortive. It showed that conflicts of genome A and C in hybrid induced abnormal meioses of meiocytes.

Key words: breeding barriers, interspecific hybridization, crossability, hybrid sterility, Oryza sativa, Oryza officinalis

FU Xue-lin, LU Yong-gen, LIU Xiang-dong, LI Jin-quan , ZHAO Xing-juan. Cytological Behavior of Hybridization Barriers Between Oryza sativa and Oryza officinalis[J]. Journal of Integrative Agriculture, 2011, 10(10): 1489-1500.

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Cytological Behavior of Hybridization Barriers Between Oryza sativa and Oryza officinalis

Cytological Behavior of Hybridization Barriers Between Oryza sativa and Oryza officinalis

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