Identification and Gene Mapping of a multi-floret spikelet 1 (mfs1) Mutant Associated with Spikelet Development in Rice

doi:10.1016/S1671-2927(00)8690

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (10): 1574-1579.DOI: 10.1016/S1671-2927(00)8690

Identification and Gene Mapping of a multi-floret spikelet 1 (mfs1) Mutant Associated with Spikelet Development in Rice

REN De-yong**, LI Yun-feng**, WANG Zeng, XU Fang-fang, GUO Shuang, ZHAO Fang-ming, SANG Xianchun, LING ing-hua, HE Guang-hua

1.Key Laboratroy of Application and Safety Control of Genetically Modified Crops, Rice Research Institute, Southwest University, Chongqing 400716, P.R.China

收稿日期:2011-06-27 出版日期:2012-10-01 发布日期:2012-11-12
通讯作者: Correspondence HE Guang-hua, Tel: +86-23-68250158, E-mail: hegh1968@yahoo.com.cn
基金资助:
This work was supported by funds from the National Natural Science Foundation of China (31071071), the Major Research Projects of Chongqing, China (CSTC, 2010AA1013), the Doctor Foundation of Southwest University, China (SWU110017), the Fundamental Research Funds for the Central Universities, China (XDJK2010C073).

Identification and Gene Mapping of a multi-floret spikelet 1 (mfs1) Mutant Associated with Spikelet Development in Rice

REN De-yong*, LI Yun-feng*, WANG Zeng, XU Fang-fang, GUO Shuang, ZHAO Fang-ming, SANG Xianchun, LING ing-hua, HE Guang-hua

1.Key Laboratroy of Application and Safety Control of Genetically Modified Crops, Rice Research Institute, Southwest University, Chongqing 400716, P.R.China

Received:2011-06-27 Online:2012-10-01 Published:2012-11-12
Contact: Correspondence HE Guang-hua, Tel: +86-23-68250158, E-mail: hegh1968@yahoo.com.cn
Supported by:
This work was supported by funds from the National Natural Science Foundation of China (31071071), the Major Research Projects of Chongqing, China (CSTC, 2010AA1013), the Doctor Foundation of Southwest University, China (SWU110017), the Fundamental Research Funds for the Central Universities, China (XDJK2010C073).

摘要/Abstract

摘要： In this study, a rice spikelet mutant, multi-floret spikelet 1 (mfs1), which was derived from ethylmethane sulfonate (EMS)- treated Jinhui 10 (Oryza sativa L. ssp. indica) exhibited pleiotropic defects in spikelet development. The mfs1 spikelet displayed degenerated the empty glume, elongated the rachilla, the extra lemma-like organ and degraded the palea. Additionally, mfs1 flowers produced varied numbers of inner floral organs. The genetic analysis revealed that the mutational trait was controlled by a single recessive gene. With 401 recessive individuals from the F2 segregation population, the MFS1 gene was finally mapped on chromosome 5, an approximate 350 kb region. The present study will be useful for cloning and functional analysis of MFS1, which would facilitate understanding of the molecular mechanism involved in spikelet development in rice.

关键词: empty glume , gene mapping , mfs1 mutant , rice (Oryza sativa L.) , spikelet

Abstract: In this study, a rice spikelet mutant, multi-floret spikelet 1 (mfs1), which was derived from ethylmethane sulfonate (EMS)- treated Jinhui 10 (Oryza sativa L. ssp. indica) exhibited pleiotropic defects in spikelet development. The mfs1 spikelet displayed degenerated the empty glume, elongated the rachilla, the extra lemma-like organ and degraded the palea. Additionally, mfs1 flowers produced varied numbers of inner floral organs. The genetic analysis revealed that the mutational trait was controlled by a single recessive gene. With 401 recessive individuals from the F2 segregation population, the MFS1 gene was finally mapped on chromosome 5, an approximate 350 kb region. The present study will be useful for cloning and functional analysis of MFS1, which would facilitate understanding of the molecular mechanism involved in spikelet development in rice.

Key words: empty glume , gene mapping , mfs1 mutant , rice (Oryza sativa L.) , spikelet

REN De-yong*, LI Yun-feng*, WANG Zeng, XU Fang-fang, GUO Shuang, ZHAO Fang-ming, SANG Xianchun, LING ing-hua, HE Guang-hua. Identification and Gene Mapping of a multi-floret spikelet 1 (mfs1) Mutant Associated with Spikelet Development in Rice[J]. Journal of Integrative Agriculture, 2012, 12(10): 1574-1579.

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Identification and Gene Mapping of a multi-floret spikelet 1 (mfs1) Mutant Associated with Spikelet Development in Rice

Identification and Gene Mapping of a multi-floret spikelet 1 (mfs1) Mutant Associated with Spikelet Development in Rice

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