Identification of Quantitative Trait Locus for Overwintering Germinability in Rice (Oryza sativa L.)

doi:10.1016/S1671-2927(00)8711

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (11): 1767-1774.DOI: 10.1016/S1671-2927(00)8711

Identification of Quantitative Trait Locus for Overwintering Germinability in Rice (Oryza sativa L.)

ZHAO Zheng-wu, LUO An-cai, LE Tao , LI Shi-gui

1.School of Life Sciences, Chongqing Normal University, Chongqing 400047, P.R.China
2.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China

收稿日期:2011-07-19 出版日期:2012-11-01 发布日期:2012-11-24
通讯作者: Correspondence LI Shi-gui, Tel: +86-28-82722661, E-mail: lishigui@263.com
作者简介:ZHAO Zhen-wu, Tel: +86-23-65910315, E-mail: zhaozhengwu513@sina.com
基金资助:
This research was supported by the Rice Key Project of Chongqing, China (CSTC2011AB1076), the Foundation Project of Chongqing Education Commission (KJ110623), the Doctor Foundation of Chongqing Normal University, China (10LR028) and the Plants Breeding Project of Chongqing, China (CSTC2010AA1033).

Identification of Quantitative Trait Locus for Overwintering Germinability in Rice (Oryza sativa L.)

ZHAO Zheng-wu, LUO An-cai, LE Tao , LI Shi-gui

1.School of Life Sciences, Chongqing Normal University, Chongqing 400047, P.R.China
2.Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China

Received:2011-07-19 Online:2012-11-01 Published:2012-11-24
Contact: Correspondence LI Shi-gui, Tel: +86-28-82722661, E-mail: lishigui@263.com
About author:ZHAO Zhen-wu, Tel: +86-23-65910315, E-mail: zhaozhengwu513@sina.com
Supported by:
This research was supported by the Rice Key Project of Chongqing, China (CSTC2011AB1076), the Foundation Project of Chongqing Education Commission (KJ110623), the Doctor Foundation of Chongqing Normal University, China (10LR028) and the Plants Breeding Project of Chongqing, China (CSTC2010AA1033).

摘要/Abstract

摘要： 312 recombinant inbred lines (RILs) in F9 from a cross between a overwintering cold-tolerant germplasm resource Glutinous rice 89-1(Gr 89-1) and a cold-sensitive variety Shuhui 527 was used for quantitative trait locus (QTL) analysis. The scores of percent ratooning germinability (PRG) and overwintering germinability (POG) were evaluated. The overwintering germination rate of axillary buds was scored to represent the overwintering germinability. Two significant QTLs (qPRG-4 and qPRG-7) on chromosomes 4 and 7 were detected and explained 8.3 and 7.2% of the total phenotypic variation, respectively. Three significant QTLs (qPOG-2, qPOG-3 and qPOG-7) were identified and mapped on chromosomes 2, 3, and 7, respectively. These QTLs contributed 9.6, 6.7, and 17.8% of phenotypic variations, respectively. A comparative analysis using SSR markers closely linked to the three QTLs for the overwintering revealed cold-tolerant individuals, which harbour the Glutinous rice 89-1 alleles at RM7110, RM250, RM418, and RM232, had a high percent overwintering germinability, while cold-sensitive individuals, which carry Shuhui 527 alleles at these loci, had a low percent overwintering germinability in the F2 population of Shuhui 527/Glutinous rice 89-1. This study demonstrated the utility of these SSR markers for selection of overwintering germinability genotypes.

关键词: rice (Oryza sativa L.), percent overwintering germinability, recombinant inbred lines, QTL analysis, coldtolerance

Abstract: 312 recombinant inbred lines (RILs) in F9 from a cross between a overwintering cold-tolerant germplasm resource Glutinous rice 89-1(Gr 89-1) and a cold-sensitive variety Shuhui 527 was used for quantitative trait locus (QTL) analysis. The scores of percent ratooning germinability (PRG) and overwintering germinability (POG) were evaluated. The overwintering germination rate of axillary buds was scored to represent the overwintering germinability. Two significant QTLs (qPRG-4 and qPRG-7) on chromosomes 4 and 7 were detected and explained 8.3 and 7.2% of the total phenotypic variation, respectively. Three significant QTLs (qPOG-2, qPOG-3 and qPOG-7) were identified and mapped on chromosomes 2, 3, and 7, respectively. These QTLs contributed 9.6, 6.7, and 17.8% of phenotypic variations, respectively. A comparative analysis using SSR markers closely linked to the three QTLs for the overwintering revealed cold-tolerant individuals, which harbour the Glutinous rice 89-1 alleles at RM7110, RM250, RM418, and RM232, had a high percent overwintering germinability, while cold-sensitive individuals, which carry Shuhui 527 alleles at these loci, had a low percent overwintering germinability in the F2 population of Shuhui 527/Glutinous rice 89-1. This study demonstrated the utility of these SSR markers for selection of overwintering germinability genotypes.

Key words: rice (Oryza sativa L.), percent overwintering germinability, recombinant inbred lines, QTL analysis, coldtolerance

ZHAO Zheng-wu, LUO An-cai, LE Tao , LI Shi-gui. Identification of Quantitative Trait Locus for Overwintering Germinability in Rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2012, 12(11): 1767-1774.

ZHAO Zheng-wu, LUOAn-cai , LE Tao , LI Shi-gui. Identification of Quantitative Trait Locus for Overwintering Germinability in Rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2012, 12(11): 1767-1774.

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Identification of Quantitative Trait Locus for Overwintering Germinability in Rice (Oryza sativa L.)

Identification of Quantitative Trait Locus for Overwintering Germinability in Rice (Oryza sativa L.)

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