Overexpression of a Cytosolic Ascorbate Peroxidase Gene, OsAPX2, Increases Salt Tolerance in Transgenic Alfalfa

doi:10.1016/S2095-3119(13)60691-7

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (11): 2500-2507.DOI: 10.1016/S2095-3119(13)60691-7

Overexpression of a Cytosolic Ascorbate Peroxidase Gene, OsAPX2, Increases Salt Tolerance in Transgenic Alfalfa

ZHANG Qian, MA Cui, XUE Xin, XU Ming, LI Jing , WU Jin-xia

1、Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2、College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730000, P.R.China

收稿日期:2013-08-01 出版日期:2014-11-06 发布日期:2014-11-14
通讯作者: WU Jin-xia, Tel: +86-10-82106133, Fax: +86-10-82106132, E-mail: wujinxia@caas.cn
作者简介:ZHANG Qian, E-mail: springzhang55@163.com
基金资助:
This work was supported by the National 973 Program of China (2014CB138700).

Overexpression of a Cytosolic Ascorbate Peroxidase Gene, OsAPX2, Increases Salt Tolerance in Transgenic Alfalfa

ZHANG Qian, MA Cui, XUE Xin, XU Ming, LI Jing , WU Jin-xia

1、Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2、College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730000, P.R.China

Received:2013-08-01 Online:2014-11-06 Published:2014-11-14
Contact: WU Jin-xia, Tel: +86-10-82106133, Fax: +86-10-82106132, E-mail: wujinxia@caas.cn
About author:ZHANG Qian, E-mail: springzhang55@163.com
Supported by:
This work was supported by the National 973 Program of China (2014CB138700).

摘要/Abstract

摘要： Alfalfa (Medicago sativa L.) is an important forage crop in the world and it is of great significance for the improvement of its salt tolerance. To improve salt tolerance in alfalfa, a rice ascorbate peroxidase gene (OsAPX2) was introduced into alfalfa using Agrobacterium tumefaciens-mediated transformation with marker gene bar. The different T-DNA insertions in T1 transgenic alfalfa were identified by Southern hybridization. Three independent T2 transgenic lines were selected for stress analysis and the results showed that all of them were salt tolerant compared with wild-type plants. The transgenic plants had low levels of H2O2, malondialdehyde and relative electrical conductivity under salt and drought stresses. Moreover, the contents of chlorophyll and proline, and APX activity were high in transgenic plants under salt and drought stresses. Taken together, the overexpression of OsAPX2 enhances salt tolerance in alfalfa through scavenging reactive oxygen species.

关键词: ascorbate peroxidase (APX) , rice (Oryza sativa L.) , salt tolerance , alfalfa

Abstract: Alfalfa (Medicago sativa L.) is an important forage crop in the world and it is of great significance for the improvement of its salt tolerance. To improve salt tolerance in alfalfa, a rice ascorbate peroxidase gene (OsAPX2) was introduced into alfalfa using Agrobacterium tumefaciens-mediated transformation with marker gene bar. The different T-DNA insertions in T1 transgenic alfalfa were identified by Southern hybridization. Three independent T2 transgenic lines were selected for stress analysis and the results showed that all of them were salt tolerant compared with wild-type plants. The transgenic plants had low levels of H2O2, malondialdehyde and relative electrical conductivity under salt and drought stresses. Moreover, the contents of chlorophyll and proline, and APX activity were high in transgenic plants under salt and drought stresses. Taken together, the overexpression of OsAPX2 enhances salt tolerance in alfalfa through scavenging reactive oxygen species.

Key words: ascorbate peroxidase (APX) , rice (Oryza sativa L.) , salt tolerance , alfalfa

ZHANG Qian, MA Cui, XUE Xin, XU Ming, LI Jing , WU Jin-xia. Overexpression of a Cytosolic Ascorbate Peroxidase Gene, OsAPX2, Increases Salt Tolerance in Transgenic Alfalfa[J]. Journal of Integrative Agriculture, 2014, 13(11): 2500-2507.

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Overexpression of a Cytosolic Ascorbate Peroxidase Gene, OsAPX2, Increases Salt Tolerance in Transgenic Alfalfa

Overexpression of a Cytosolic Ascorbate Peroxidase Gene, OsAPX2, Increases Salt Tolerance in Transgenic Alfalfa

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