Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (8): 1827-1834.DOI: 10.1016/S2095-3119(13)60537-7

• 论文 • 上一篇    下一篇

Identification of QTLs Underlying Folate Content in Milled Rice

 DONG Wei, CHENG Zhi-jun, XU Jian-long, ZHENG Tian-qing, WANG Xiao-le, ZHANG Hong-zheng, WANG Jie , WAN Jian-min   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
  • 收稿日期:2013-05-20 出版日期:2014-09-22 发布日期:2014-09-24
  • 通讯作者: WAN Jian-min, Tel: +86-10-62186628, E-mail: wanjm@caas.cn
  • 作者简介:DONG Wei, Mobile: 18810707913, Tel: +86-10-82108786, E-mail: sdu_dw@163.com; CHENG Zhi-jun, Tel: +86-10-82108786, E-mail: chengzhijun@caas.cn
  • 基金资助:

    This work was supported by grants from the National Basic Research Program of China (2007CB10880-1, 2013CB127000).

Identification of QTLs Underlying Folate Content in Milled Rice

 DONG Wei, CHENG Zhi-jun, XU Jian-long, ZHENG Tian-qing, WANG Xiao-le, ZHANG Hong-zheng, WANG Jie , WAN Jian-min   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
  • Received:2013-05-20 Online:2014-09-22 Published:2014-09-24
  • Contact: WAN Jian-min, Tel: +86-10-62186628, E-mail: wanjm@caas.cn
  • About author:DONG Wei, Mobile: 18810707913, Tel: +86-10-82108786, E-mail: sdu_dw@163.com; CHENG Zhi-jun, Tel: +86-10-82108786, E-mail: chengzhijun@caas.cn
  • Supported by:

    This work was supported by grants from the National Basic Research Program of China (2007CB10880-1, 2013CB127000).

PDF

摘要： Understanding the genetic mechanism underlying folate biosynthesis and accumulation in rice would be beneficial for breeding high folate content varieties as a cost-effective approach to addressing widespread folate deficiency in developing countries. In this study, the inheritance of rice grain folate content was investigated in the Lemont/Teqing recombinant inbred lines and the Koshihikari/Kasalath//Koshihikari backcross inbred lines. 264 F12 recombinant inbred lines (RILs) and 182 BC1F10 backcross inbred lines (BILs) with their parents planted in randomized complete blocks with two replicates in 2010, and RILs harvested in 2008 were used for QTL detection using inclusive composite interval mapping (ICIM) method. In the RIL population, two QTLs, denoted by qQTF-3-1 and qQTF-3-2 (QTF, quantitative total folate), explaining 7.8% and 11.1-15.8% of the folate content variation were detected in one or two years, respectively. In the BIL population, a QTL, denoted by qQTF-3-3, was detected, explaining 25.3% of the variation in folate content. All the positive alleles for higher folate content were from the high-folate parents, i.e., Teqing and Kasalath. The known putative folate biosynthesis genes do not underlie the QTLs detected in this study and therefore may be novel loci affecting folate content in milled rice. QTLs identified in this study have potential value for marker assisted breeding for high-folate rice variety.

关键词: rice , recombinant inbred lines , backcross inbred lines , quantitative trait locus , folate content

Abstract: Understanding the genetic mechanism underlying folate biosynthesis and accumulation in rice would be beneficial for breeding high folate content varieties as a cost-effective approach to addressing widespread folate deficiency in developing countries. In this study, the inheritance of rice grain folate content was investigated in the Lemont/Teqing recombinant inbred lines and the Koshihikari/Kasalath//Koshihikari backcross inbred lines. 264 F12 recombinant inbred lines (RILs) and 182 BC1F10 backcross inbred lines (BILs) with their parents planted in randomized complete blocks with two replicates in 2010, and RILs harvested in 2008 were used for QTL detection using inclusive composite interval mapping (ICIM) method. In the RIL population, two QTLs, denoted by qQTF-3-1 and qQTF-3-2 (QTF, quantitative total folate), explaining 7.8% and 11.1-15.8% of the folate content variation were detected in one or two years, respectively. In the BIL population, a QTL, denoted by qQTF-3-3, was detected, explaining 25.3% of the variation in folate content. All the positive alleles for higher folate content were from the high-folate parents, i.e., Teqing and Kasalath. The known putative folate biosynthesis genes do not underlie the QTLs detected in this study and therefore may be novel loci affecting folate content in milled rice. QTLs identified in this study have potential value for marker assisted breeding for high-folate rice variety.

Key words: rice , recombinant inbred lines , backcross inbred lines , quantitative trait locus , folate content