QTLMapping forAdult-Plant Resistance to Stripe Rust in a CommonWheat RIL Population Derived from Chuanmai 32/Chuanyu 12

doi:10.1016/S1671-2927(00)8712

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (11): 1775-1782.DOI: 10.1016/S1671-2927(00)8712

QTLMapping forAdult-Plant Resistance to Stripe Rust in a CommonWheat RIL Population Derived from Chuanmai 32/Chuanyu 12

WU Ling, XIA Xian-chun, ZHENG You-liang, ZHANG Zheng-yu, ZHU Hua-zhong, LIU Yong-jian, YANG En-nian, LI Shi-zhao , HE Zhong-hu

1.Crop Research Institute, Sichuan Academy of Agricultural Science, Chengdu 610066, P.R.China
2.Institute of Crop Sciences, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, P.R.China
3.Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
4.International Maize and Wheat Improvement Center (CIMMYT) China Office/Chinese Academy of Agricultural Sciences, Beijing 100081,P.R.China
5.Plant Protection Institute, Sichuan Academy of Agricultural Science, Chengdu 610066, P.R.China
6.College of Agriculture, Sichuan Agricultural University, Chengdu 611130, P.R.China

收稿日期:2011-07-30 出版日期:2012-11-01 发布日期:2012-11-24
通讯作者: Correspondence HE Zhong-hu, Tel: +86-10-82108547, E-mail: zhhecaas@163.com
基金资助:
This study was supported by the National Natural Science Foundation of China (30821140351), the Crop Breeding Foundation of Sichuan Province, China, the Special Treasury Foundation in Genetic Engineering of Sichuan Province and an Earmarked Fund for the Modern Agro-industry Technology Research System, China.

QTLMapping forAdult-Plant Resistance to Stripe Rust in a CommonWheat RIL Population Derived from Chuanmai 32/Chuanyu 12

WU Ling, XIA Xian-chun, ZHENG You-liang, ZHANG Zheng-yu, ZHU Hua-zhong, LIU Yong-jian, YANG En-nian, LI Shi-zhao , HE Zhong-hu

1.Crop Research Institute, Sichuan Academy of Agricultural Science, Chengdu 610066, P.R.China
2.Institute of Crop Sciences, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, P.R.China
3.Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
4.International Maize and Wheat Improvement Center (CIMMYT) China Office/Chinese Academy of Agricultural Sciences, Beijing 100081,P.R.China
5.Plant Protection Institute, Sichuan Academy of Agricultural Science, Chengdu 610066, P.R.China
6.College of Agriculture, Sichuan Agricultural University, Chengdu 611130, P.R.China

Received:2011-07-30 Online:2012-11-01 Published:2012-11-24
Contact: Correspondence HE Zhong-hu, Tel: +86-10-82108547, E-mail: zhhecaas@163.com
Supported by:
This study was supported by the National Natural Science Foundation of China (30821140351), the Crop Breeding Foundation of Sichuan Province, China, the Special Treasury Foundation in Genetic Engineering of Sichuan Province and an Earmarked Fund for the Modern Agro-industry Technology Research System, China.

摘要/Abstract

摘要： Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a devastating wheat disease worldwide. The Chinese wheat cultivar Chuanmai 32 has shown stable resistance to stripe rust for 10 yr in Sichuan Province, a hotspot for stripe rust epidemics. The objective of the present study was to map quantitative trait loci (QTL) for adult-plant resistance (APR) to stripe rust in a population of 140 recombinant inbred lines (RILs) derived from Chuanmai 32/Chuanyu 12. Field trials were conducted in Chengdu and Yaan, Sichuan, from 2005 to 2008, providing stripe rust reaction data for 6 environments. 797 simple sequence repeat (SSR) markers were screened for association with stripe rust reaction, initially through bulked segregant analysis (BSA). Based on the mean disease values averaged across environments, the broad-sense heritability of maximum disease severity (MDS) was 0.75. Two QTLs for stripe rust resistance were detected by composite interval mapping (CIM). They were designated QYr.caas-3BL and QYr.caas-3BS and explained from 6.6 to 20.1%, respectively, of the phenotypic variance across environments. QYr.caas-3BL came from Chuanmai 32; QYr.caas-3BS with lower effect was from the susceptible parent Chuanyu 12. Both QTLs appear to be new.

关键词: APR, Triticum aestivum, microsatellites, quanitative trait locus, yellow rust

Abstract: Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a devastating wheat disease worldwide. The Chinese wheat cultivar Chuanmai 32 has shown stable resistance to stripe rust for 10 yr in Sichuan Province, a hotspot for stripe rust epidemics. The objective of the present study was to map quantitative trait loci (QTL) for adult-plant resistance (APR) to stripe rust in a population of 140 recombinant inbred lines (RILs) derived from Chuanmai 32/Chuanyu 12. Field trials were conducted in Chengdu and Yaan, Sichuan, from 2005 to 2008, providing stripe rust reaction data for 6 environments. 797 simple sequence repeat (SSR) markers were screened for association with stripe rust reaction, initially through bulked segregant analysis (BSA). Based on the mean disease values averaged across environments, the broad-sense heritability of maximum disease severity (MDS) was 0.75. Two QTLs for stripe rust resistance were detected by composite interval mapping (CIM). They were designated QYr.caas-3BL and QYr.caas-3BS and explained from 6.6 to 20.1%, respectively, of the phenotypic variance across environments. QYr.caas-3BL came from Chuanmai 32; QYr.caas-3BS with lower effect was from the susceptible parent Chuanyu 12. Both QTLs appear to be new.

Key words: APR, Triticum aestivum, microsatellites, quanitative trait locus, yellow rust

WULing, XIAXian-chun, ZHENGYou-liang, ZHANGZheng-yu, ZHUHua-zhong, LIUYong-jian, YANG En-nian, LI Shi-zhao , HE Zhong-hu. QTLMapping forAdult-Plant Resistance to Stripe Rust in a CommonWheat RIL Population Derived from Chuanmai 32/Chuanyu 12[J]. Journal of Integrative Agriculture, 2012, 12(11): 1775-1782.

WULing , XIAXian-chun , ZHENGYou-liang , ZHANGZheng-yu , ZHUHua-zhong , LIUYong-jian , YANG En-nian, LI Shi-zhao , HE Zhong-hu. QTLMapping forAdult-Plant Resistance to Stripe Rust in a CommonWheat RIL Population Derived from Chuanmai 32/Chuanyu 12[J]. Journal of Integrative Agriculture, 2012, 12(11): 1775-1782.

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QTLMapping forAdult-Plant Resistance to Stripe Rust in a CommonWheat RIL Population Derived from Chuanmai 32/Chuanyu 12

QTLMapping forAdult-Plant Resistance to Stripe Rust in a CommonWheat RIL Population Derived from Chuanmai 32/Chuanyu 12

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