十种柚类及柚杂种果实中类黄酮含量的超高效液相色谱分析

doi:10.3864/j.issn.0578-1752.2013.09.017

中国农业科学 ›› 2013, Vol. 46 ›› Issue (9): 1892-1902.doi: 10.3864/j.issn.0578-1752.2013.09.017

十种柚类及柚杂种果实中类黄酮含量的超高效液相色谱分析

方波, 赵其阳, 席万鹏, 周志钦, 焦必宁

1.西南大学园艺园林学院，重庆 400716
2.南方山地园艺学教育部重点实验室，重庆 400715
3.农业部柑橘产品质量安全风险评估实验室/西南大学柑桔研究所，重庆 400712
4.国家柑桔工程技术研究中心，重庆 400712

收稿日期:2012-12-28 出版日期:2013-05-01 发布日期:2013-02-28
通讯作者: 通信作者焦必宁，E-mail：bljiao@tom.com；通信作者周志钦，E-mail：zzqswu @yahoo.com
作者简介:方波，Tel：15902366380；E-mail：fb0211@hotmail.com
基金资助:
农业部国家现代农业（柑橘）产业技术体系建设专项（CARS-27）、国家“948”计划项目（2012-Z13）、国家自然科学基金项目（31171930）、中央高校基本科研业务费专项（XDJK2013A014）

Determination of Flavonoids in 10 Pummelo and Pummelo Hybrid Fruits by Ultra Performance Liquid Chromatography

FANG Bo, ZHAO Qi-Yang, XI Wan-Peng, ZHOU Zhi-Qin, JIAO Bi-Ning

1.College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716
2.Key Laboratory of Horticulture Science for Southern Mountainous Regions，Ministry of Education, Chongqing 400715
3.Laboratory of Risk Assessment for Citrus Quality and Safety of Ministry of Agriculture/Citrus Research Institute, Southwest University, Chongqing 400712
4.National Citrus Engineering Research Center, Chongqing 400712

Received:2012-12-28 Online:2013-05-01 Published:2013-02-28

摘要/Abstract

摘要： 【目的】利用超高效液相色谱法同时检测10种柚及柚杂种果实中11种类黄酮含量，测定分析各品种果实类黄酮的种类及其含量变化规律。【方法】超高效液相色谱法，色谱柱：ACQUITY UPLC BEH C18 分析柱（2.1 mm×100 mm，1.7 μm）；流动相：0.1%乙酸水溶液（A）和甲醇（B），采用梯度洗脱，洗脱程序为：（1） 0—5 min，90%—80% A；（2）5—10 min，80%—30% A；（3）10—15 min，30%—20% A；（4）15—16 min，20%—90% A；柱温：35℃；流速：0.3 mL•min-1。【结果】类黄酮组分及含量与柑橘的遗传基因型有关，柚果实中类黄酮以柚皮苷为主，葡萄柚果实中以柚皮苷和新橙皮苷为主。柚皮苷在瑞红中含量最高（果皮：27.5 mg•g-1 DW，果肉：2.73 mg•g-1 DW），新橙皮苷在鸡尾中含量最高（果皮：14.93 mg•g-1 DW，果肉：0.70 mg•g-1 DW）。杂种后代类黄酮累积受其亲本影响。【结论】超高效液相色谱法是一种定性定量柑橘果实类黄酮的有效方法，柚果实中类黄酮含量丰富且各种类之间组分含量差异显著。

关键词: 超高效液相色谱 , 柚 , 类黄酮 , 测定

Abstract: 【Objective】This study aimed to determine 11 flavonoids in 10 pummelos and pummelo hybrids fruits and analyze flavonoids types and content in varieties of fruits by ultra performance liquid chromatograph (UPLC) method.【Method】The separation was carried on a ACQUITY UPLC BEH C18 (2.1 mm×100 mm，1.7 μm, column temperature 35℃). Methanol (A)/0.1% formic acid/water (B) mixture was used as the mobile phase at a flow rate of 0.3 mL•min-1 through gradient elution: (1) 0-5 min, 90%-80% A; (2) 5-10 min, 80%-30% A; (3) 10-15 min, 30%-20% A; (4) 15-16 min, 20%-90% A. 【Result】 The flavonoids types and content in citrus fruit depend on genetic genotypes. Naringin is a major flavonoid in pummel. Naringin and neohesperidin are major flavonoids in grapefruit. Naringin content is higher in Riored fruit (Peel: 27.5 mg•g-1 DW, Pulp: 2.73 mg•g-1 DW) and neohesperidin content is higher in Cocktail (Peel: 14.93 mg•g-1 DW, Pulp: 0.70 mg•g-1 DW). The accumulation of flavonoids in hybrid fruits were affected by their parents.【Conclusion】UPLC method was effective in qualitative and quantitative analysis of flavonoids in citrus fruits. The flavonoids were abundant in pummelo fruits and the difference of flavonoids composition in diverse citrus fruits was significant.

Key words: ultra performance liquid chromatography , pummelo , flavonoids , determination

方波, 赵其阳, 席万鹏, 周志钦, 焦必宁. 十种柚类及柚杂种果实中类黄酮含量的超高效液相色谱分析[J]. 中国农业科学, 2013, 46(9): 1892-1902.

FANG Bo, ZHAO Qi-Yang, XI Wan-Peng, ZHOU Zhi-Qin, JIAO Bi-Ning. Determination of Flavonoids in 10 Pummelo and Pummelo Hybrid Fruits by Ultra Performance Liquid Chromatography[J]. Scientia Agricultura Sinica, 2013, 46(9): 1892-1902.

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十种柚类及柚杂种果实中类黄酮含量的超高效液相色谱分析

Determination of Flavonoids in 10 Pummelo and Pummelo Hybrid Fruits by Ultra Performance Liquid Chromatography

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