黄瓜-酸黄瓜染色体片段导入系群体的构建及果实相关数量性状基因的定位

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doi:10.3864/j.issn.0578-1752.2012.08.011

摘要： 【目的】以栽培黄瓜（Cucumis sativus L. , 2n = 14)‘北京截头’为受体亲本，以野生酸黄瓜( C. hystrix Chakr, 2n = 24）为供体亲本，采用SSR标记辅助选择法构建黄瓜-酸黄瓜染色体片段导入系群体。初步定位控制黄瓜果实外形的数量性状基因。【方法】首先通过种间杂交-回交-自交获得大量的染色体片段导入系株系。然后选择均匀分布在黄瓜染色体组上的298对SSR标记对亲本进行多态性检测，使用检测出的亲本间差异引物对染色体片段导入系株系进行检测，筛选含有野生酸黄瓜染色体片段的植株。对该群体果实外形进行初步调查，利用t测验与轮回亲本比较，鉴定QTL。【结果】本研究构建了由50个株系组成的染色体片段导入系群体。在该群体中共检测到149个染色体导入片段，包含61个不同的导入片段，不同导入片段的总长度为259.95 cM，基因组覆盖率为45.37%。导入片段的长度在1.65—15.4 cM，平均长度为5.41 cM，分布于黄瓜的7条染色体上。利用该导入系群体初步定位了控制黄瓜果型的13个QTL。【结论】构建了一套以栽培黄瓜为背景，野生酸黄瓜为前景的染色体片段导入系群体，并利用该导入系初步定位了控制黄瓜果型的QTL，为开发利用野生酸黄瓜的优良基因提供了新的种质资源，也为今后定位黄瓜的数量性状遗传位点奠定了材料基础。

关键词: 野生酸黄瓜, 染色体导入系, 分子标记辅助选择, 数量性状位点

Abstract: 【Objective】 A set of cucumber-sour cucumber chromosome introgression lines were constructed by using cultivated cucumber (Cucumis sativus L., 2n=14) cultivar ‘Beijingjietou’ as recurrent parent and the wild ‘sour cucumber’ (C. hystrix Chakr., 2n = 24) as donor parent by the way of marker-assisted selection, and QTL controlling cucumber fruit shape was detected initially. 【Method】 Fristly, through interspecific-backcrossing-selfing approach, a lot of cucumber-sour cucumber chromosome introgression lines were obtained. Then polymorphism of the parents was detected by 298 pairs of SSR markers which uniformly distributed in the cucumber genome. And then the cucumber-sour cucumber chromosome introgression lines which contained the wild sour cucumber chromosome segments from the strains were screened by using the detected differences between parents SSR markers. QTL analysis of three main traits affecting cucumber fruit shape(fruit length, fruit diameter, fruit length/diameter ratio) was conducted using t-test compared with the recurrent parent. 【Result】 A set of 50 cucumber-sour cucumber chromosome introgression lines was constructed. There are 149 chromosome introgression segments containing 61 different introgression segments were identified in the lines. The total length of the different segments was 259.95 cM, and the coverage was 45.37% in the cucumber genome. The length of the introgression segments ranged from 1.65 cM to 15.4 cM with an average of 5.41 cM. They distributed in 7 chromosomes of cucumber. A total of 13 QTL affecting cucumber fruit shape were detected in CSIL population initially. 【Conclusion】 In this research, a set of cucumber-sour cucumber introgression lines were created and QTL affecting cucumber fruit shape was detected initially. It provided new resources for utilization of genes from C. hystrix and also the material basis for fine mapping of quantitative traits in cucumber.

Key words: wild sour cucumber, chromosome introgression lines, marker-assisted selection, quantitative trait locus

孟佳丽, 娄群峰, 周晓慧, 史建磊, 陈劲枫. 黄瓜-酸黄瓜染色体片段导入系群体的构建及果实相关数量性状基因的定位[J]. 中国农业科学, 2012, 45(8): 1558-1567.

MENG Jia-Li, LOU Qun-Feng, ZHOU Xiao-Hui, SHI Jian-Lei, CHEN Jin-Feng. Construction of Cucumber-Sour Cucumber Chromosome Introgression Lines and Location of Fruit Related QTLs[J]. Scientia Agricultura Sinica, 2012, 45(8): 1558-1567.

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