关键词: 猪, 微卫星, QTL定位, 血常规指标

Abstract:

【Objective】 The study was performed for detecting and mapping quantitative trait loci (QTL) affecting haematological traits on the swine chromosome 10. 【Method】 A pig resource population consisted 368 individuals of three breeds (Landrace, Large White and Songliao Black Pig) distributed in 16 boar families. Thirteen microsatellite markers on swine chromosome 10 were selected and a linear mixed model was used to detect quantitative trait loci (QTL) affecting 18 haematological traits including white blood cell count (WBC), and blood cell (RBC), and blood platelet counts (PLT). Then, the method of substitution was used to calculate significant threshold values by likelihood ratio test. 【Result】 Thirteen microsatellite markers of this population were the genetic markers of medium polymorphism. On average, the number of alleles was 3.1754, heterozygosity was 0.5215, and polymorphic information content was 0.5999, shannon index was 1.3222, respectively, during all microsatellite markers of three breeds. Four significant QTL (P＜0.05) were identified on chromosome 10 affecting HCT, HGB, MCV and PLT, respectively. 【Conclusion】 Four QTLs affecting haematological traits were found to be located at 81-136 cM on chromosome 10. The markers close by were SW249, SWR136, S0070, and SW1894.

Key words: swine, microsatellite, QTL mapping, haematological traits