中国农业科学 ›› 2010, Vol. 43 ›› Issue (5): 1023-1030 .doi: 10.3864/j.issn.0578-1752.2010.05.018

• 贮藏·保鲜·加工 • 上一篇    下一篇

DNA分子标记技术在酿酒酵母菌株遗传多样性分析中的应用

裴颖芳,刘延琳

  

  1. (西北农林科技大学葡萄酒学院/陕西省葡萄-葡萄酒工程中心)
  • 收稿日期:2009-07-20 修回日期:2009-08-12 出版日期:2010-03-01 发布日期:2010-03-01
  • 通讯作者: 刘延琳

Application of DNA Molecular Markers in Genetic Diversities of Saccharomyces cerevisiae Strains

PEI Ying-fang, LIU Yan-lin   

  1. (西北农林科技大学葡萄酒学院/陕西省葡萄-葡萄酒工程中心)
  • Received:2009-07-20 Revised:2009-08-12 Online:2010-03-01 Published:2010-03-01
  • Contact: LIU Yan-lin

摘要:

本文介绍了RAPD、mtDNA-RFLP、微卫星DNA(microsatellite)、Interdelta指纹图谱以及线粒体DNA COX1基因指纹图谱等5种DNA分子标记技术的方法和原理及其优缺点,同时探讨了DNA分子标记技术在国内外酿酒酵母菌株区分中的应用状况与前景。

关键词: 酿酒酵母菌株, RAPD, mtDNA-RFLP, interdelta指纹图谱, COX1 基因, 微卫星DNA

Abstract:

The basic principles of the methods concerning five DNA molecular markers including random amplified polymorphic DNA (RAPD), fragment length polymorphism of mitochondrial DNA (mtDNA-RFLP), microsatellite DNA, interdelta fingerprinting and mtDNA COX1 gene fingerprinting were introduced in this paper,including illustration of both the advantages and the disadvantages of each method. Besides, the application and prospect of these DNA molecular markers in differentiation of Saccharomyces cerevisiae strains were discussed and reviewed.

Key words: Saccharomyces cerevisiae strains, RAPD, mtDNA-RFLP, interdelta fingerprinting, COX1 gene, micro-satellite DNA