关键词: 中华蜜蜂, 工蜂繁殖, 工蜂监督, 微卫星DNA, 人工授精

Abstract: Abstract: 【Objective】 For exploring the effect of worker policing in Apis cerana cerana, the worker reproduction was studied in different colonies. 【Method】 Apis cerana cerana was the studied biology in this paper. The virgin queens were instrumentally inseminated with 1 or 2 males, and the controlling ones were naturally mated. 7 weeks after the queens reproduced successfully, the drones were detected whether developed from the unfertilized eggs laid by queen or by workers, which based on the technology of honeybee microsatellite DNA that sampled the pupae of worker and drone in every colony. 【Result】 The drones were developed from the unfertilized eggs laid by queen in all of the colonies. 【Conclusion】 The activity of worker policing are taking place universally in colonies which the queens were instrumentally inseminated and naturally mated.

Key words: Apis cerana cerana, Worker reproduction, Worker policing, Microsatellite DNA, Artificial inseminated