关键词: 豌豆, 机械伤害, 质膜ATP酶, 防御反应

Abstract:

【Objective】 Effects of plasma membrane H+-ATPase (PM H+-ATPase) on defense response to mechanical wounding were investigated. 【Method】 Using pea (Pisum sativum L.)seedlings as materials, treated by mechanical wounding, and using methods of enzymes activity measures, western blot detection and electron microscope cytochemistry, the effects of mechanical wounding on PM H+-ATPase activities were observed. Furthermore, the relationship between PM H+-ATPase and accumulation of active oxygen in inducing wounding defense response was studied. 【Result】 Mechanical wounding induced the increasing of PM H+-ATPase activity, but did not change its content; suppressed PM H+-ATPase activity, inhibited H2O2 accumulation which has been induced by wounding and also inhibited the phenylalanine ammonia lyase activity's increasing, while application of active oxygen scavengers had no effect on the wounding -induced PM H+-ATPase activity increasing. 【Conclusion】It is thus suggested that PM H+-ATPase participated in signal transduction of defense response to mechanical wounding, which acted on the upstream of reactive oxygen signal and induced the formation of defense response on the downstream by protein phosphorylation regulation.

Key words: pea (Pisum sativum L.), mechanical wounding, plasma membrane H＋-ATPase, defense response