中国农业科学 ›› 2007, Vol. 40 ›› Issue (9): 2045-2052 .

• 园艺 • 上一篇    下一篇

三种黑色粮油作物种皮花色苷提取物抗氧化能力的稳定性比较

李莉蓉,张名位,刘邻渭,魏振承,张瑞芬   

  1. 广东省农业科学院生物技术研究所/农业部功能食品重点开放实验室
  • 收稿日期:2005-12-02 修回日期:1900-01-01 出版日期:2007-09-10 发布日期:2007-09-10
  • 通讯作者: 张名位

Comparative Study on the Antioxidant Stability of Anthocyanin Extracts in Seed Coats of Three Black Cereal and Oil Crops

  

  1. 广东省农业科学院生物技术研究所/农业部功能食品重点开放实验室
  • Received:2005-12-02 Revised:1900-01-01 Online:2007-09-10 Published:2007-09-10

摘要: 【目的】比较外界因素及杀菌工艺对黑米、黑大豆、黑玉米3种黑色粮油作物种皮花色苷提取物总抗氧化能力的影响。【方法】选用不同光照、温度、食品原料、防腐剂、金属离子和5种杀菌工艺(巴氏灭菌、煮沸灭菌、高温短时灭菌、高压蒸汽灭菌、微波灭菌、)处理3种花色苷提取物,采用铁离子还原法评价其处理前后的总抗氧化能力变化。【结果】黑米、黑大豆、黑玉米种皮花色苷提取物的总抗氧化能力随光照和加热时间延长而下降,温度越高下降越快,在避光、自然光和日光灯照射3种光照条件下黑米花色苷提取物的稳定性最好,其次为黑大豆花色苷提取物的,黑玉米花色苷提取物的最差;在相同温度下黑大豆花色苷提取物的稳定性最好,黑米和黑玉米花色苷提取物的较差;NaCl、蔗糖、葡萄糖等食品原料和防腐剂苯甲酸钠对3种花色苷提取物总抗氧化能力的影响不明显,5种金属离子(K+、Ca2+、Mg2+、Zn2+、Cu2+)和5种不同杀菌工艺(巴氏灭菌、煮沸灭菌、高温短时灭菌、高压蒸汽灭菌、微波灭菌)对其总抗氧化能力均有不同程度的影响,其中高温短时灭菌的影响较小,高压蒸汽灭菌的影响最大。【结论】黑米、黑大豆、黑玉米3种花色苷提取物抗氧化稳定性受光照、温度、食品原料、金属离子和杀菌工艺等多种因素影响。

关键词: 黑米, 黑大豆, 黑玉米, 花色苷, 抗氧化作用, 稳定性

Abstract: The effects of external factors and five sterilizing processes on the stabilities of total antioxidant capacities (TAC) in three anthocyanin extracts of black rice, black soybean and black corn by colorimetry and ferric reducing ability method were investigated. The results showed that the TAC of anthocyanin extracts from seed coats of black rice, black soybean and black corn displayed the photosensitive characteristics that decreased with the prolonging of illumination; under the conditions of natural light, fluorescent light, and without illumination, the TAC stability was in the order of black rice anthocyanin (BRA), black soybean anthocyanin (BSA), and black corn anthocyanin (BCA); the TAC of these three anthocyanin extracts displayed the heat sensitive characteristics that decreased with the prolonging of heating time, and the higher the temperature, the more they were decreased; the TAC stability was in the order of BSA, BRA and BCA. There were no significant effects of food materials such as NaCl, sucrose, and glucose and preservative sodium benzoate on the TAC stabilities of these three anthocyanin extracts, while there were different effects of five metal ions of K+, Ca2+, Mg2+, Zn2+, and Cu2+ on the TAC stabilities of these three anthocyanin extracts; the TAC stabilities of these three anthocyanin extracts were significantly affected by five sterilizing processes of boiling sterilization, autoclave sterilization, pasteurization, microwave sterilization, and high temperature short time sterilization, of which they were least affected by high temperature short time treatment, and most affected by autoclave sterilization.

Key words: Anthocyanin, antioxidation, Stability