中国农业科学 ›› 2007, Vol. 40 ›› Issue (7): 1573-1578 .

• 研究简报 • 上一篇    

猪卵母细胞透明带弹性系数—杨氏模量测定

卢晟盛,宋立峰,滕维中,杨绿峰,卢克焕   

  1. 广西亚热带生物资源保护利用重点实验室
  • 收稿日期:2005-10-19 修回日期:2005-12-30 出版日期:2007-07-10 发布日期:2007-07-10
  • 通讯作者: 卢克焕

The Measurement of Elasticity Coefficient--Young’s Modulus of the Zona Pellucida of Porcine Oocytes

  

  1. 广西亚热带生物资源保护利用重点实验室
  • Received:2005-10-19 Revised:2005-12-30 Online:2007-07-10 Published:2007-07-10

摘要: 【目的】为了探讨猪卵母细胞的生物物理特性,该文研究了猪卵母细胞透明带的力学性质。【方法】在39℃环境下,利用微管吸吮的方法在倒置显微镜下通过显微操作装置用显微玻璃管吸吮猪卵母细胞,通过自制的专用于卵母细胞透明带杨氏模量测量软件,测量了经过44~48 h体外成熟培养的核成熟和核未成熟猪卵母细胞(n=76,共7次重复)的透明带杨氏模量。【结果】计算得到猪卵母细胞透明带的弹性模量为(1.43±0.29)×104Pa。【结论】该研究结合了生殖生物学和力学的内容,为评判哺乳动物卵母细胞和胚胎质量开拓了新的思路和内容。

关键词: 猪, 卵母细胞, 透明带, 微管吸吮, 杨氏模量

Abstract: The mechanics property of the zona pellucida of porcine oocytes was investigated in this study. Under 39℃ conditions, the Young’s modulus of the zona pellucida, of porcine oocytes (n=76, 7 replicates in total) cultured in vitro for 44-48 hours and of which nuclear matured and not matured, were measured. The elasticity modulus of the zona pellucida of these oocytes is (1.43±0.29)×104 Pa. Also an computer software for measuring the Young’s modulus of the zona pellucida of oocytes was made.

Key words: porcine, oocyte, zona pellucida, micropipette aspiration, Young’s Modulus