关键词: 甜菜, 单胚雄性不育系, 单胚保持系, 形态学, 细胞学

Abstract: The differences of biological and anatomic characteristic of abortive pollen in sugar beet between male sterile line and maintenance line were examined in the laboratory of Sugar Beet Research Institute. The development phase and process of bud and pollen stained with paraffin slicer and Ai-shi haematine crystal, safranine and fast green in the three group monogerm sugar beets with male sterile line and maintenance line were observed under the light microscope. The results showed that there were significant differences between the two lines in the flower structure. The pale green concave anther could be seen clearly before flowering; and the anther without curl changed into white or pale green in the maintenance line after flowering. But for male sterile line, anther and the pollen abnormally developed, and the abortive courses can be divided into four phases, which were mainly that tapetum and middle-layer separated during tetrad period, development of microspore stopped at the stage of its forming and tapetum and microspore gradually disappeared after conglutination, those resulted in the anther drying and shriveling, the pollen was abortive in the end.

Key words: Sugar beet, Male sterile line of monogerm, Abortive pollen, Maintenance line, Cytology