中国农业科学 ›› 2007, Vol. 40 ›› Issue (7): 1395-1402 .

• 植物保护 • 上一篇    下一篇

葡萄根癌生防菌株E26生防效果的离体检测方法研究

王 靖,郭岩彬,王建辉,李金云,王慧敏   

  1. 中国农业大学植物病理学系
  • 收稿日期:2006-04-27 修回日期:1900-01-01 出版日期:2007-07-10 发布日期:2007-07-10
  • 通讯作者: 王慧敏,李金云

Screening of the method in vitro for testing the effect of E26 against grape crown gall

Jing Wang Yanbin Guo JinYun Li JianHui Wang HuiMin Wang   

  1. 中国农业大学植物病理学系
  • Received:2006-04-27 Revised:1900-01-01 Online:2007-07-10 Published:2007-07-10
  • Contact: Jing Wang

摘要: 【目的】筛选葡萄离体茎段和叶片的最适表面消毒条件、离体培养基和接种方法,建立生防菌株E26防治葡萄根癌病效果的离体检测方法。【方法】通过比较6种表面消毒方法,4种培养基和2种接种方法,建立最优的E26生防效果离体检测方法;并通过与温室、大田生防效果的比较以及瘤组织切片观察、冠瘿碱纸电泳检测验证该离体检测方法的准确性。【结果】 在1/2 B5培养基的基础上改良出适合于葡萄离体茎段培养用的MSC 1/2B5培养基;筛选出葡萄离体茎段的最佳消毒条件和接种方法,建立了检测E26生防效果的离体茎段接种法。该方法的检测结果与葡萄活体植株的检测结果基本一致,能够快速准确地反映E26的防效;并且,瘤组织切片观察和冠瘿碱纸电泳检测结果证实了该离体检测方法的可靠性。叶圆片法检测时,根癌瘤只能在葡萄叶圆片的叶脉处形成,瘤组织较小,难以准确反映E26的生防效果。【结论】本研究建立的离体茎段检测E26的生防效果是一种比较理想的离体检测E26生防效果的方法。

关键词: 葡萄根癌病, 生防效果, 离体检测

Abstract: Grape crown gall disease is a soil-borne bacterial disease caused by virulent strain of A.vitis. Biocontrol bacteria has been one of the efficient methods to control the disease. Evaluation of biocontrol effect is extremely important in screening of potential biocontrol agent. At present, the effect testing is performed by inoculating indicator plant or host plant in vivo. In this communication, we investigated the effect of E26 through inoculating grape stem and leaf in vitro. 1/2B5 medium was modified for better growth of grape stem in vitro culture, and the best methods of sterilization and inoculation were screened in this experiment. The stem in vitro test demonstrated the same effect when compared with the in vivo test, and could be the quick and accurate evaluation to indicate the effect of E26. While the grape leaf in vitro test could not accurately reflect the effect of E26, because crown galls formed only on leaf veins and were too small to be examined. The grape stem in vitro testing can be a promising method to detect the effect of E26.

Key words: grape crown gall, Biocontrol effect, testing In vitro