中国农业科学 ›› 2007, Vol. 40 ›› Issue (11): 2474-2480 .

• 植物保护 • 上一篇    下一篇

水稻瘤矮病毒基因组第6片段(S6)序列测定与分析

刘福秀,阮小蕾,何云蔚,李华平   

  1. 华南农业大学植物病毒研究室
  • 收稿日期:2006-11-27 修回日期:1900-01-01 出版日期:2007-11-10 发布日期:2007-11-10
  • 通讯作者: 李华平

Sequencing and analysis of Rice gall dwarf virus genome segment 6

  1. 华南农业大学植物病毒研究室
  • Received:2006-11-27 Revised:1900-01-01 Online:2007-11-10 Published:2007-11-10

摘要: 【目的】了解水稻瘤矮病毒(RGDV)基因组结构与功能。【方法】根据已报道的RGDV基因组各片段末端序列及其反向重复特性,采用4轮RT-PCR的方法扩增RGDV基因组第6片段(S6),用生物学软件进行序列分析。【结果】得到了该病毒S6的全序列。S6全长1 651 bp,G+C含量39.13%,包含一个长的ORF,起始于第25位,终止于第1 497位,编码490个氨基酸,分子量为53.3 kD。 【结论】该蛋白与伤瘤病毒(WTV)的P7的相似性为30.0%,与水稻矮缩病毒(RDV)的P7的相似性为31.7%。这是RGDV S6全序列的首次报道。

关键词: 植物呼肠孤病毒, 水稻瘤矮病毒(RGDV), 基因组第6片段(S6), 序列分析

Abstract: 【Objective】In order to understand the whole genome organization and its functions of Rice gall dwarf virus (RGDV), we got the complete sequence of RGDV genome segment 6. 【Method】Four rounds of RT-PCR were performed according to the property of segment-specific reverted repeat termini sequences reported. Sequences were analyzed by using bio-software. 【Result】The full-length cDNA of S6 was 1 651 bp with 39.13% G+C content, containing a single open reading frame (ORF) that extends 1473 nucleotides from nucleotide 25. The single ORF encoded a putative 53.3 kDa polypeptide, named Psn6, containing 490 amino acids. 【Conclusion】 RGDV Psn6 exhibited 31.7% amino acid sequence identity to P7 of Rice dwarf virus (RDV), and 30.0% to P7 of Wound tumor virus (WTV). This is the first report of the complete sequence of RGDV S6.

Key words: Phytoreovirus, Rice gall dwarf virus (RGDV), segment 6, sequence analysis