中国农业科学 ›› 2006, Vol. 39 ›› Issue (10): 2118-2123 .

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

兔脑nNOS阳性神经元的形态、结构和分布规律

尹逊河,王树迎,蔡玉梅,邱建华,王传宝,刘燕   

  1. 山东农业大学动物科技学院
  • 收稿日期:2005-05-30 修回日期:1900-01-01 出版日期:2006-10-10 发布日期:2006-10-10
  • 通讯作者: 尹逊河 xhyin

Modality Structure and Distributed the law of nNOSPositive Neurons in Rabbit Brain

XunHe Yin,,,,,   

  1. 山东农业大学动物科技学院
  • Received:2005-05-30 Revised:1900-01-01 Online:2006-10-10 Published:2006-10-10
  • Contact: XunHe Yin

摘要: 利用SABC免疫组织化学方法,对兔脑神经型一氧化氮合酶(nNOS)阳性神经元的形态、结构及分布规律进行了系统研究,结果表明:① nNOS阳性神经元呈深棕色,着色主要在胞质内,细胞核处较为浅淡;神经元胞体形态多种多样,有三角形、圆形、椭圆形、梭形等,神经元突起有一个或者数个;nNOS阳性神经纤维大多呈棕色串珠样,有些区域的阳性纤维交错分布,相互交织成网状。②家兔的各个脑区均有nNOS阳性神经元和神经纤维出现,其中在大脑皮质、小脑、丘脑下部、中脑和脑桥广泛分布,延髓分布极少。③nNOS阳性神经元,在仔兔时就已基本发育到成年兔水平,以后随着年龄增长逐渐发育成熟至衰老,到2岁龄(老年兔)时就出现了显著的衰老变化,即密度减小,表达强度减弱,第一级突起数减少,最长突起长度变短等。结果提示:nNOS阳性神经元及其催化产生的NO在家兔神经系统的发育和神经调控中起重要作用。

关键词: 兔脑, 神经型一氧化氮合酶, 阳性神经元,

Abstract: The study,using SABC Immune histochemistry technique,was designed for observing the modality,structure and distributed the law of Neuronal Nitric oxide Synthase (nNOS ) positive neurons in rabbit brain. The results showed:①The nNOS positive neurons appeared deep brown which had most in the Cytoplasm and the cell nucleus were more lighter;the neuron cell bodies had all kinds of types,such as triangle,round,ellipse,shuttle and so on;the neuron protruding had one or more;the most positive nerve fibres were brown bunch of pearls kind,some nNOS postive fibres were netted. ② The nNOS positive neurons and nerve fibres were distributed in every area of rabbit brain. And they were widely distributed in cerebral cortex,cerebellum,hypothalamus,mesencephalon and pons. But there were even fewer in medulla . ③Basically,the nNOS positive neurons of the young rabbit had developed to the adult rabbit's level. It developed from maturation to caducity gradually with age. To two years old(aged rabbit), the nNOS positive neurons appeared senescence obviously,including the reducing of density, weakening of expression intensity,decrease of the first born tuber number and shortening of the longest born tuber length etc. The results indicated that nNOS positive neurons and the NO which nNOS positive neurons catalyzed did most use in the nervous system and the nerve adjusting and controlling.

Key words: Rabbit brain, Neuronal Nitric oxide Synthase (nNOS), Positive neurons, Structure, Distributed the law Immune histochemistry histochemical