关键词: 中生菌素, 水稻悬浮细胞, 苯丙氨酸解氨酶基因, 转录表达

Abstract: The transcript accumulation of phenylalanine ammonialyase gene of the suspension-cultured cells in the near-isogenic rice lines, which were treated by Zhongshengmycin, Xanthomonas oryzae pv. oryzae(Xoo), and Zhongshengmycin+ Xoo, respectively, was studied. The results showed that these different treatments could induce the transcript accumulation of phenylalanine ammonialyase gene in the rice suspension-cultured cells at different degrees. CBB4, the resistant rice line to Xoo, the transcript accumulation of the enzyme gene was induced at 3 h after treating with Zhongshengmycin, and inducing transcript lasted to 48 h in largely. Although the transcript accumulation was induced at 3 h after inoculating Xoo, reached the peak at 6 h and inducing transcript lasted to 48 h, the transcript accumulation was less. For Shennong 1033, the susceptible rice line to Xoo, the transcript accumulation of phenylalanine ammonialyase gene was induced from 3 h to 48 h after treating with Zhongshengmycin. While the inducing transcript was only accumulated at 12 h to 24 h after inoculating Xoo. The inducing degree and mode of transcript accumulation of phenylalanine ammonialyase gene treated with Zhongshengmycin+ Xoo were similar to Zhongshengmycin treatment.

Key words: Zhongshengmycin, Rice suspension-cultured cells, Phenylalanine ammonialyase gene, Transcript accumulation