三叶斑潜蝇对冷驯化的响应及不同种群耐寒性差异

doi:10.3864/j.issn.0578-1752.2021.13.008

摘要/Abstract

摘要：

【目的】研究重大入侵害虫三叶斑潜蝇（Liriomyza trifolii）对快速冷驯化和长时冷驯化的响应以及3个地理种群（湖北省武汉市、海南省海口市和安徽省安庆市）的耐寒性,为明确该虫在我国高纬度地区定殖的潜在可能性提供参考。【方法】将海南三叶斑潜蝇种群蛹和成虫在-12、-14、-16、-18、-20、-22和-24℃低温暴露30 min,选择存活率为15%—30%的温度为识别温度。设置5℃为快速冷驯化的驯化温度,12℃为长时冷驯化的驯化温度,将三叶斑潜蝇蛹和成虫置于5℃下1—6 h或置于12℃下1—6 d,再置于识别温度下30 min检测存活率,未驯化的蛹和成虫也置于识别温度下30 min作为对照,比较其冷驯化后低温存活率的差异。另外,设置5个靶标温度,分别为0、-5、-10、-15和-20℃,将3个地理种群的三叶斑潜蝇蛹在靶标温度下冷暴露2 h,比较其存活率差异。最后,利用过冷却点测定仪对3个地理种群蛹的过冷却点（supercooling point,SCP）进行测定。【结果】三叶斑潜蝇蛹和成虫暴露于-20℃下30 min后,其存活率分别为15.0%、19.6%、21.0% (介于15%—30%),因此将-20℃确定为三叶斑潜蝇的冷驯化识别温度。短时间的5℃低温暴露提高了三叶斑潜蝇蛹和成虫的耐寒性,成虫对快速冷驯化的响应更加积极。1 h和2 h的冷驯化效果最好,随着暴露时间的延长,其驯化效果会逐渐减弱直至消失。在6 d内,不同时长的12℃低温暴露均会提高三叶斑潜蝇蛹和成虫的耐寒性且经历不同时长冷驯化后其耐寒性差异较小。在5、0、-5℃的低温暴露下,海南种群与安徽、湖北种群蛹的存活率差异不显著,在-10、-15、-20℃的低温暴露下,海南种群蛹的存活率显著低于安徽和湖北种群。安徽(-22.19℃)和湖北(-22.19℃) 种群蛹的过冷却点显著低于海南种群(-21.06℃)。【结论】三叶斑潜蝇的耐寒性可以通过快速冷驯化或长时冷驯化获得增强,这可能是三叶斑潜蝇逐步向我国高纬度地区扩散的原因之一。湖北和安徽种群表现出的耐寒性较海南种群强。研究结果有助于预测三叶斑潜蝇在我国的越冬分布区域,指导其监测预警及防控。

关键词: 三叶斑潜蝇, 冷驯化, 过冷却点, 耐寒性

Abstract:

【Objective】The response of the major invasive pest Liriomyza trifolii to cold acclimation and the cold tolerance of three geographical populations (Wuhan City, Hubei Province; Haikou City, Hainan Province; Anqing City, Anhui Province) were studied to provide references for the potential colonization of L. trifolii in high latitudes of China.【Method】The pupae and adults of L. trifolii population in Hainan were exposed to low temperatures of -12, -14, -16, -18, -20, -22 and -24℃ for 30 min, and the temperature with a survival rate of 15%-30% was selected as the recognition temperature. The L. trifolii pupae and adults were put at 5℃ for 1-6 h or 12℃ for 1-6 d, and then put at the recognition temperature for 30 min to detect the survival rate. The undomesticated pupae and adults were also placed at the recognition temperature for 30 min as a control, and the difference in low-temperature survival rate after cold acclimation was compared. Besides, five target temperatures were set at 0, -5, -10, -15, and -20℃, respectively, and the pupae of three geographical populations were exposed to the target temperatures for 2 h, the difference of survival rate was studied. Finally, the supercooling point (SCP) of pupae of three geographical populations was measured by supercooling point tester.【Result】When theL. trifolii pupae and adults were exposed to -20℃ for 30 min, the survival rates were 15.0%, 19.6%, and 21.0%, respectively. Therefore, -20℃ was determined as the cold acclimation recognition temperature of L. trifolii. The cold tolerance ofL. trifolii pupae and adults was improved after rapid cold acclimation at 5℃, and the adults responded more positively to rapid cold acclimation. The effect of cold acclimation for 1 h and 2 h was the best, and the effect of rapid cold acclimation decreased gradually until disappeared with the extension of exposure time. Within 6 days, the cold tolerance ofL. trifolii pupae and adults was improved by low-temperature exposure at 12℃ for different periods, and there was little difference in cold tolerance after cold acclimation for different periods. Besides, under the low-temperature exposure of 5, 0 and -5℃, there was no significant difference in the survival rate of pupae between the Hainan population and Anhui, Hubei populations, but under the low-temperature exposure of -10, -15 and -20℃, the pupa survival rate of Hainan population was significantly lower than that of Anhui and Hubei populations. The SCP of pupae in the Anhui and Hubei populations was significantly lower than that in the Hainan population.【Conclusion】The cold tolerance of L. trifolii can be enhanced by cold acclimation, which may be one of the reasons why L. trifolii gradually spread to high latitudes in China. The cold tolerance of the Hubei and Anhui populations is stronger than that of the Hainan population. The results are helpful to predict the overwintering distribution area of L. trifolii in China and guide its monitoring, early warning, and prevention and control.

Key words: Liriomyza trifolii, cold acclimation, supercooling point, cold tolerance

张起恺,邢振龙,吴圣勇,徐瑞瑞,雷仲仁. 三叶斑潜蝇对冷驯化的响应及不同种群耐寒性差异[J]. 中国农业科学, 2021, 54(13): 2781-2788.

ZHANG QiKai,XING ZhenLong,WU ShengYong,XU RuiRui,LEI ZhongRen. Response of Liriomyza trifolii to Cold Acclimation and Differences of Cold Tolerance Among Different Populations[J]. Scientia Agricultura Sinica, 2021, 54(13): 2781-2788.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2021.13.008

https://www.chinaagrisci.com/CN/Y2021/V54/I13/2781

图/表 5

图1

图2

图3

图4

图5

参考文献 38

[1]	雷仲仁, 姚君明, 朱灿建, 王海鸿. 三叶斑潜蝇在中国的适生区预测. 植物保护, 2007, 33(5):100-103.
	LEI Z R, YAO J M, ZHU C J, WANG H H. Prediction of suitable areas for Liriomyza trifolii (Burgess) in China . Plant Protection, 2007, 33(5):100-103. (in Chinese)
[2]	JOHNSON M W, WELTER S C, TOSCANO N C, TING I P, TRUMBLE J T. Reduction of tomato leaflet photosynthesis rates by mining activity ofLiriomyza sativae(Diptera: Agromyzidae). Journal of Economic Entomology, 1983, 76(5):1061-1063. doi: 10.1093/jee/76.5.1061
[3]	PARRELLA M P, JONES V P, YOUNGMAN R R, LEBECK L M. Effect of leaf mining and leaf stippling ofLiriomyzaspp.on photosynthetic rates ofChrysanthemum. Annals of the Entomological Society of America, 1985, 78(1):90-93. doi: 10.1093/aesa/78.1.90
[4]	REITZ S R, TRUMBLE J T. Interspecific and intraspecific differences in twoLiriomyza leafminer species in California. Entomologia Experimentalis et Applicata, 2002, 102(2):101-113. doi: 10.1046/j.1570-7458.2002.00930.x
[5]	王宪辉, 齐宪磊, 康乐. 昆虫的快速冷驯化现象及其生态适应意义. 自然科学进展, 2003, 13(11):1128-1133.
	WANG X H, QI X L, KANG L. The phenomenon of rapid cold acclimation of insects and its significance of ecological adaptation. Progress in Natural Science, 2003, 13(11):1128-1133. (in Chinese)
[6]	KIM Y, KIM N. Cold hardiness inSpodoptera exigua(Lepidoptera: Noctuidae). Environmental Entomology, 1997, 26(5):1117-1123. doi: 10.1093/ee/26.5.1117
[7]	OVERGAARD J, MALMENDAL A, SØRENSEN J G, BUNDY J G, LOESCHCKE V, NIELSEN N C, HOLMSTRUP M. Metabolomic profiling of rapid cold hardening and cold shock in Drosophila melanogaster. Journal of Insect Physiology, 2007, 53(12):1218-1232. doi: 10.1016/j.jinsphys.2007.06.012
[8]	WANG X H, KANG L. Rapid cold hardening in young hoppers of the migratory locustLocusta migratoriaL. (Orthoptera: Acridiidae). Cryo Letters, 2003, 24(5):331-340.
[9]	SINCLAIR B J, CHOWN S L. Rapid cold-hardening in a Karoo beetle,Afrinussp. Physiological Entomology, 2006, 31(1):98-101. doi: 10.1111/pen.2006.31.issue-1
[10]	KANG L, CHEN B, WEI J N, LIU T X. Roles of thermal adaptation and chemical ecology in Liriomyza distribution and control. Annual Review of Entomology, 2009, 54:127-145. doi: 10.1146/annurev.ento.54.110807.090507
[11]	SHINTANI Y, ISHIKAWA Y. Relationship between rapid cold- hardening and cold acclimation in the eggs of the yellow-spotted longicorn beetle, Psacothea hilaris. Journal of Insect Physiology, 2007, 53(10):1055-1062. doi: 10.1016/j.jinsphys.2007.05.012
[12]	孔璐, 郭建英, 周忠实, 万方浩. 昆虫冷驯化机制研究进展. 应用昆虫学报, 2012, 49(6):1664-1669.
	KONG L, GUO J Y, ZHOU Z S, WAN F H. Progress in research on cold hardening in insects. Chinese Journal of Applied Entomology, 2012, 49(6):1664-1669. (in Chinese)
[13]	TURNOCK W J, BOIVIN G, RING R A. Interpopulation differences in the coldhardiness of Delia radicum (Diptera: Anthomyiidae). The Canadian Entomologist, 1998, 130(2):119-129. doi: 10.4039/Ent130119-2
[14]	SHINTANI Y, ISHIKAWA Y. Geographic variation in cold hardiness of eggs and neonate larvae of the yellow-spotted longicorn beetle Psacothea hilaris. Physiological Entomology, 1999, 24(2):158-164. doi: 10.1046/j.1365-3032.1999.00126.x
[15]	TANAKA K. Cold tolerance in the house spider,Achaearanea tepidariorum (Araneae: Theridiidae). Entomological Science, 1999, 2(4):597-604.
[16]	JING X H, KANG L. Geographical variation in egg cold hardiness: A study on the adaptation strategies of the migratory locust Locusta migratoriaL. Ecological Entomology, 2003, 28(2):151-158. doi: 10.1046/j.1365-2311.2003.00497.x
[17]	OVERGAARD J, HOFFMANN A A, KRISTENSEN T N. Assessing population and environmental effects on thermal resistance in Drosophila melanogaster using ecologically relevant assays. Journal of Thermal Biology, 2011, 36(7):409-416. doi: 10.1016/j.jtherbio.2011.07.005
[18]	CHEN B, KANG L. Variation in cold hardiness of Liriomyza huidobrensis (Diptera: Agromyzidae) along latitudinal gradients. Environmental Entomology, 2004, 33(2):155-164. doi: 10.1603/0046-225X-33.2.155
[19]	CHEN B, KANG L. Can greenhouses eliminate the development of cold resistance of the leafminers? Oecologia, 2005, 144(2):187-195. doi: 10.1007/s00442-005-0051-2
[20]	相君成, 雷仲仁, 王海鸿, 高玉林. 三种外来入侵斑潜蝇种间竞争研究进展. 生态学报, 2012, 32(5):1616-1622.
	XIANG J C, LEI Z R, WANG H H, GAO Y L. Interspecific competition among three invasiveLiriomyzaspecies . Acta Ecologica Sinica, 2012, 32(5):1616-1622. (in Chinese)
[21]	WAN F H, YANG N W. Invasion and management of agricultural alien insects in China. Annual Review of Entomology, 2016, 61:77-98. doi: 10.1146/annurev-ento-010715-023916
[22]	PARRELLA M P. Biology of Liriomyza. Annual Review of Entomology, 1987, 32:201-224. doi: 10.1146/annurev.en.32.010187.001221
[23]	XING Z L, LIU Y Q, CAI W Z, HUANG X Z, WU S Y, LEI Z R. Efficiency of trichome-based plant defense in Phaseolus vulgaris depends on insect behavior, plant ontogeny, and structure. Frontiers in Plant Science, 2017, 8:2006. doi: 10.3389/fpls.2017.02006
[24]	ZHAO Y X, KANG L. Cold tolerance of the leafminerLiriomyza sativae (Dipt., Agromyzidae). Journal of Applied Entomology, 2000, 124(3/4):185-189. doi: 10.1046/j.1439-0418.2000.00463.x
[25]	WANG H H, LEI Z R, LI X, OETTING R D. Rapid cold hardening and expression of heat shock protein genes in the B-biotypeBemisia tabaci. Environmental Entomology, 2011, 40(1):132-139. doi: 10.1603/EN09357
[26]	张智, 郑乔, 张云慧, 刘杰, 殷新田, 汤清波, 李静, 袁源, 李祥瑞, 朱勋. 草地贪夜蛾室内种群抗寒能力测定. 植物保护, 2019, 45(6):43-49.
	ZHANG Z, ZHENG Q, ZHANG Y H, LIU J, YIN X T, TANG Q B, LI J, YUAN Y, LI X R, ZHU X. Cold hardiness of laboratory populations of Spodoptera frugiperda. Plant Protection, 2019, 45(6):43-49. (in Chinese)
[27]	CHEN C P, DENLINGER D L, LEE JR R E. Cold-shock injury and rapid cold hardening in the flesh fly Sarcophaga crassipalpis. Physiological Zoology, 1987, 60(3):297-304. doi: 10.1086/physzool.60.3.30162282
[28]	DANKS H V. The wider integration of studies on insect cold- hardiness. European Journal of Entomology, 1996, 93:383-404.
[29]	RAKO L, HOFFMANN A A. Complexity of the cold acclimation response in Drosophila melanogaster. Journal of Insect Physiology, 2006, 52(1):94-104. doi: 10.1016/j.jinsphys.2005.09.007
[30]	JENSEN D, OVERGAARD J, SØRENSEN J G. The influence of developmental stage on cold shock resistance and ability to cold-harden in Drosophila melanogaster. Journal of Insect Physiology, 2007, 53(2):179-186. doi: 10.1016/j.jinsphys.2006.11.008
[31]	WANG X H, KANG L. Differences in egg thermotolerance between tropical and temperate populations of the migratory locust Locusta migratoria (Orthoptera: Acridiidae). Journal of Insect Physiology, 2005, 51(11):1277-1285. doi: 10.1016/j.jinsphys.2005.07.010
[32]	邢振龙. 三叶斑潜蝇在我国的入侵扩散及其与美洲斑潜蝇的竞争取代[D]. 北京: 中国农业大学, 2018.
	XING Z L. Range expansion of Liriomyza trifolii (Burgess) and competitive displacement with its’ congener species,L. sativae Blanchard, in China [D]. Beijing: China Agricultural University, 2018. (in Chinese)
[33]	CHEN B, KANG L. Implication of pupal cold tolerance for the northern over-wintering range limit of the leafminer Liriomyza sativae (Diptera: Agromyzidae) in China. Applied Entomology and Zoology, 2005, 40(3):437-446. doi: 10.1303/aez.2005.437
[34]	MASAKI S. Geographical variation of life cycle in crickets (Ensifera: Grylloidea). European Journal of Entomology, 1996, 93:281-302.
[35]	KIMURA M T. Cold and heat tolerance of drosophilid flies with reference to their latitudinal distributions. Oecologia, 2004, 140(3):442-449. doi: 10.1007/s00442-004-1605-4
[36]	MORGAN D J W, REITZ S R, ATKINSON P W, TRUMBLE J T. The resolution of Californian populations of Liriomyza huidobrensis and Liriomyza trifolii (Diptera: Agromyzidae) using PCR. Heredity, 2000, 85(1):53-61. doi: 10.1046/j.1365-2540.2000.00731.x
[37]	FULLER S J, CHAVIGNY P, LAPCHIN L, VANLERBERGHE- MASUTTI F. Variation in clonal diversity in glasshouse infestations of the aphid,Aphis gossypiiGlover in southern France. Molecular Ecology, 1999, 8(11):1867-1877. doi: 10.1046/j.1365-294x.1999.00782.x
[38]	HOFFMANN A A, HERCUS M J. Environmental stress as an evolutionary force. Bioscience, 2000, 50(3):217-226. doi: 10.1641/0006-3568(2000)050[0217:ESAAEF]2.3.CO;2