中国农业科学 ›› 2014, Vol. 47 ›› Issue (18): 3618-3632.doi: 10.3864/j.issn.0578-1752.2014.18.010
李培武,张奇,丁小霞,白艺珍
收稿日期:
2014-03-25
修回日期:
2014-05-27
出版日期:
2014-09-16
发布日期:
2014-09-16
通讯作者:
李培武,Tel:027-86812943;E-mail:peiwuli@oilcrops.cn
作者简介:
李培武,Tel:027-86812943;E-mail:peiwuli@oilcrops.cn
基金资助:
LI Pei-wu, ZHANG Qi, DING Xiao-xia, BAI Yi-zhen
Received:
2014-03-25
Revised:
2014-05-27
Online:
2014-09-16
Published:
2014-09-16
摘要: 目前,威胁食用植物性农产品质量安全的主要污染物为农药残留、生物毒素和重金属等。从这些污染物检测技术看,在样品前处理技术、确证检测技术和快速检测技术3个方面均取得了显著进展。样品前处理技术建立并改进了固相萃取、固相微萃取、超临界流体萃取、基质固相分散萃取和微波辅助萃取技术等,其中免疫亲和吸附技术和QuEChERS(Quick, Easy, Cheap, Effective, Rugged, and Safe)技术得到了快速发展和越来越广泛的应用;确证检测技术特别是色谱-质谱联用技术,因其具有灵敏、准确、可靠等优点,特别适合用作实验室仲裁检测,得到了快速发展和应用,并已列入农产品中农药残留、生物毒素等污染物测定方法标准中;农产品污染物快速检测技术方面,中国逐步实现从技术源头创新到终端产品创制的全程创新,研制出多种类、满足不同层次需求的产品,原理上既有酶抑制法,又有免疫分析法和无损检测法,形式上既有各种试剂盒、免疫亲和柱,又有各类免疫层析试纸条,为现场筛查、定性或定量测定提供了可供选择的技术方法,与大型仪器方法形成互补。从农产品污染物风险评估研究进展看,中国农产品质量安全风险评估体系建设成效显著,建成了以国家农产品质量安全风险评估机构为龙头、专业性和区域性风险评估实验室为主体、各主产区实验站为基础、生产基地观测点为延伸的具有中国特色的农产品质量安全风险评估体系,并根据6大类危害因子,提出了4大类风险评估类型,明确了各类风险评估的性质特征和评估程序,初步建立了包括农药残留、生物毒素、重金属、外源添加物等在内的农产品质量安全风险评估技术框架体系,研究开发出各类危害因子识别技术、风险评估专用软件等技术产品。从农产品污染控制研究看,农药残留、生物毒素和重金属等污染途径、规律、机制等被逐步解析,提出了一系列污染防控技术,包括污染源头控制技术、过程控制技术、消减技术等。当前存在的突出问题包括:1)农产品质量安全检测技术研究原始创新有待加强,尤其是不同种类混合污染物同步测定技术;2)风险评估研究起步晚,能力弱,社会需求迫切,基础数据不足;3)农产品危害因子污染控制基础研究薄弱,对污染发生、迁移、转化规律与机制缺少系统研究,关键控制点仍不明确,缺乏污染早期预警与防控技术。随着对食用植物性农产品质量安全研究的深入,危害因子检测技术自主创新性将会不断加强,技术方法与产品系列化将满足不同场所和层次对检测需求;通过加强对危害因子发生规律、营养品质形成与控制机理的研究,农产品风险预警与污染防控技术将得到快速发展和应用;农产品质量安全风险评估将成为农产品质量安全监管不可或缺的重要技术性、基础性工作,相关研究将成为热点,并在实现农产品质量安全多因子全过程科学管理、依法监督中发挥越来越重要的作用。
李培武,张奇,丁小霞,白艺珍. 食用植物性农产品质量安全研究进展[J]. 中国农业科学, 2014, 47(18): 3618-3632.
LI Pei-wu, ZHANG Qi, DING Xiao-xia, BAI Yi-zhen. A Review of Studies on Quality and Safety of Edible Vegetable Agro-Products[J]. Scientia Agricultura Sinica, 2014, 47(18): 3618-3632.
[1] 金发忠. 农产品质量安全监管体系基本形成. 中国食品安全报, 2011-11-15.
Jin F Z. The System Construction of quality and safety monitoring of agricultural products. China Food Safety, 2011-11-15. (in Chinese)
[2] 叶志华. 推进农产品质量安全科技创新的战略思考. 农业质量标准, 2009(6): 7-10.
Ye Z H. Thought on the science and technology innovation of quality and safety of agricultural products. Agricultural Quality and Standards, 2009(6): 7-10. (in Chinese)
[3] Guan W B, Li Z N, Zhang H Y, Hong H J, Rebeyev N, Ye Y, Ma Y Q. Amine modified graphene as reversed-dispersive solid phase extraction materials combined with liquid chromatography–tandem mass spectrometry for pesticide multi-residue analysis in oil crops. Journal of Chromatography A, 2013, 1286: 1-8.
[4] Tankiewicz M, Morrison C, Biziuk M. Multi-residue method for the determination of 16 recently used pesticides from various chemical groups in aqueous samples by using DI-SPME coupled with GC-MS. Talanta, 2013, 107: 1-10.
[5] González-Curbelo M A, Herrera-Herrera A V, Ravelo-Pérez L M, Hernández-Borges J. Sample-preparation methods for pesticide- residue analysis in cereals and derivatives. Trends in Analytical Chemistry, 2012, 38: 32-51.
[6] 程景, 李培武, 张文, 谢立华, 丁小霞, 张奇. 基质固相分散-气相色谱法测定植物油中多种拟除虫菊酯类及有机磷类农药残留. 中国油料作物学报, 2008, 30(3): 346-352.
Cheng J, Li P W, Zhang W, Xie L H, Ding X X, Zhang Q. Multiresidue determination of pesticides in vegetable oil by matrix solid-phase dispersion and gas chromatography. Chinese Journal of Oil Crops Sciences, 2008, 30(3): 346-352. (in Chinese)
[7] Satpathy G, Tyagi Y K, Gupta R K. A novel optimised and validated method for analysis of multi-residues of pesticides in fruits and vegetables by microwave-assisted extraction (MAE)–dispersive solid-phase extraction (d-SPE)–retention time locked (RTL)–gas chromatography–mass spectrometry with Deconvolution reporting software (DRS). Food Chemistry, 2011,127(3):1300-1308.
[8] 王静, 金芬, 邵华, 杨锚. 农药多残留检测样品前处理技术研究进展. 农业质量标准, 2007, 5(1): 28-32.
Wang J, Jin F, Shao H, Yang M. Research Progress on pretreatment methods for the determination of multi-pesticide residues. Agricultural Quality and Standards, 2007, 5(1): 28-32. (in Chinese)
[9] 王慧卿, 于劲松, 徐斐, 张建国. 食品农药残留检测中样品前处理技术研究进展. 广东农业科学, 2013(8): 111-114.
Wang H Q, Yu J S, Xu P, Zhang J G. Sample preparation techniques for detection of pesticide residues in foods. Guangdong Agricultural Sciences, 2013(8): 111-114. (in Chinese)
[10] 黎小鹏, 刘红梅. 水果和蔬菜中农药残留检测前处理技术研究进展. 中凯农业工程学院学报, 2013, 26(4): 1-6.
Li X P, Liu H M. Research progress on sample preparation technology in analytical procedures pesticide residue in fruits and vegetables. Journal of Zhongkai University of Agriculture and Engineering, 2013, 26(4): 1-6. (in Chinese)
[11] Wang Y, Zhang Q, Li P, Zhang W, Li Y, Ding X. Selective sample cleanup by immunoaffinity chromatography for determination of fenvalerate in vegetables. Journal of Chromatography B, 2011, 879: 3531-3537.
[12] Sanagi M M, Salleh S, Ibrahim W A W, Naim A A, Hermawan D, Miskam M, Hussain I, Aboul-Enein H Y. Molecularly imprinted polymer solid-phase extraction for the analysis of organophosphorus pesticides in fruit samples. Journal of Food Composition and Analysis, 2013, 32(2): 155-161.
[13] Hou X, Han M, Dai X H, Yang X F, Yi S G. A multi-residue method for the determination of 124 pesticides in rice by modified QuEChERS extraction and gas chromatography–tandem mass spectrometry. Food Chemistry, 2013, 138: 1198-1205.
[14] Miao Q, Kong W J, Yang S H, Yang M H. Rapid analysis of multi-pesticide residues in lotus seeds by a modified QuEChERS- based extraction and GC–ECD Original. Chemosphere, 2013, 91(7): 955-962.
[15] Steven J. Lehotay, Kyung Ae Son, Hyeyoung Kwon, Urairat Koesukwiwat, Wusheng Fu, Katerina Mastovska, Eunha Hoh, Natchanun Leepipatpiboon. Comparison of QuEChERS sample preparation methods for the analysis of pesticide residues in fruits and vegetables. Journal of Chromatography A, 2010, 1217(16): 2548-2560.
[16] Chen G Q, Cao P Y, Liu R J. A multi-residue method for fast determination of pesticides in t ea by ultra performance liquid chromatography–electrospray tandem mass spectrometry combined with modified QuEChERS sample preparation procedure. Food Chemistry, 2011, 125(4): 1406-1411.
[17] Kwon H, Lehotay S J, Geis-Asteggiante L. Variability of matrix effects in liquid and gas chromatography–mass spectrometry analysis of pesticide residues after QuEChERS sample preparation of different food crops. Journal of Chromatography A, 2012, 1270: 235-245.
[18] 姜俊, 李培武, 谢立华, 丁小霞, 李英, 王秀嫔, 汪雪芳, 张奇, 管笛. 固相萃取-全二维气相色谱/飞行时间质谱同步快速检测蔬菜中64种农药残留. 分析化学, 2011, 39(1): 72-76.
Jiang J, Li P W, Xie L H, Ding X X, Li Y, Wang X P, Wang X F, Zhang Q, Guan D. Rapid screening and simultaneous confirmation of 64 pesticide residues in vegetable using solid phase extraction- comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometer. Chinese Journal of Analytical Chemistry, 2011, 39(1): 72-76. (in Chinese)
[19] Wang X P, Li P W, Zhang W, Zhang Q, Ma F, Yu L, Wang L. Screening for pesticide residues in oil seeds using solid-phase dispersion extraction and comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry. Journal of Separation Science, 2012, 35: 1634-1643.
[20] Gómez-Ramos M M, Ferrer C, Malato O, Agüera A, Fernández- Alba A R, Miao Q, Kong W J, Yang S H, Yang M H. Liquid chromatography-high-resolution mass spectrometry for pesticide residue analysis in fruit and vegetables: Screening and quantitative studies. Journal of Chromatography A, 2013, 1287: 24-37.
[21] 叶雪珠, 王强, 赵首萍, 章虎, 王祥云, 赵燕申. 酶抑制法检测蔬菜农药残留的效果评价. 中国蔬菜, 2013(4): 76-81.
Ye X Z, Wang Q, Zhao S P, Zhang H, Wang X Y, Zhao Y S. Effect evaluation on rapid enzyme inhibiting method to detect pesticide residues in vegetable. China Vegetables, 2013(4): 76-81. (in Chinese)
[22] 董洁娴, 李振峰, 雷红涛, 何永盛, 王弘, 孙远明. 重组乙酰胆碱酯酶的表达及其分子进化研究进展. 生物工程学报, 2012, 28(5): 557-564.
Dong J X, Li Z F, Lei H T, He Y S, Wang H, Sun Y M. Expression and molecular evolution of recombinant acetylcholinesterase for detection of pesticide residues: a review. Chinese Journal of Biotechnology, 2012, 28(5): 557-564. (in Chinese)
[23] Liu Y, Lou Y, Xu D, Qian G L, Zhang Q, Wu R R, Hu B S, Liu F Q. Production and characterization of monoclonal antibody for class-specific determination of O,O-dimethyl organophosphorus pesticides and effect of heterologous coating antigens on immunoassay sensitivity. Microchemical Journal, 2009, 93(1): 36-42.
[24] Wang C M, Li X B, Liu Y H, Guo Y Y, Xie R , Gui W J, Zhu G N. Development of a mab-based heterologous immunoassay for the broad-selective determination of organophosphorus pesticides. Journal of Agricultural and Food Chemistry, 2010, 58 (9): 5658-5663.
[25] Xu Z L, Zeng D P, Yang J Y, Shen Y D, Beier R C, Lei H T, Wang H, Sun Y M. Monoclonal antibody-based broad-specificity immunoassay for monitoring organophosphorus pesticides in environmental water samples. Journal of Environmental Monitoring, 2011, 13: 3040-3048.
[26] Xu Z L, Shen Y D, Zheng W X, Beier R C, Xie G M, Dong J X, Yang J Y, Wang H, Lei H T, She Z G, Sun Y M. Broad-specificity immunoassay for O,O-Diethyl organophosphorus pesticides: application of molecular modeling to improve assay sensitivity and study antibody recognition. Analytical Chemistry, 2010, 82(22): 9314-9321.
[27] Mickova B, Kovalczuk T, Rauch P, Moreno M J, Abad A, Montoya A, Ferri E, Fini F, Girotti S. Analytical performances of validated chemiluminescent enzyme immunoassays to detect N-methylcarbamate pesticides. Analytica Chimica Acta, 2005, 528(2): 243-248.
[28] Zhang Q, Zhang W, Wang X P, Li P W. Immunoassay development for the class-specific assay for types I and II pyrethroid insecticides in water samples. Molecules, 2010, 15(1): 164-177.
[29] 张奇, 李培武, 陈小媚, 周海燕, 印南日, 甘冬生. 黄曲霉毒素免疫检测技术研究进展. 农产品质量与安全, 2013, 63(3): 42-46.
Zhang Q, Li P W, Chen X M, Zhou H Y, Yin N R, Gan D S. Research progress on technologies of immunoassay for aflatoxin. Quality and Safety of Agro-Products, 2013, 63(3): 42-46. (in Chinese)
[30] Torres-Pacheco I. Aflatoxins-Detection, Measurement and Control. Croatia: InTech, 2011.
[31] Li P W, Zhang Z W, Hu X F, Zhang Q. Advanced hyphenated chromatographic-mass spectrometry in mycotoxin determination: current status and prospects. Mass Spectrometry Reviews, 2013, 32(6): 420-452.
[32] 王秀嫔, 李培武, 杨扬, 张文, 张奇, 范素芳, 喻理, 王琳, 陈小媚, 李英, 姜俊. 液相色谱-三重串联四极杆质谱测定粮油中的黄曲霉毒素. 色谱, 2011, 29(6): 517-522.
Wang X P, Li P W, Yang Y, Zhang W, Zhang Q, Fan S F, Yu L, Wang L, Chen X M, Li Y, Jiang J. Determination of aflatoxins in cereals and oils by liquid chromatography-triple quadrupole tandem mass spectrometry. Chinese Journal of Chromatography, 2011, 29(6): 517-522. ( in Chinese)
[33] Ren Y P, Zhang Y, Shao S L, Cai Z X, Feng L, Pan H F, Wang Z G. Simultaneous determination of multi-component mycotoxin contaminants in foods and feeds by ultra-performance liquid chromatography tandem mass spectrometry. Journal of Chromatography A, 2007, 1143: 48-64.
[34] 宫小明, 任一平, 董静, 孙军, 李健, 金超, 于金玲. 超高效液相色谱串联质谱法测定花生、粮油中18种真菌毒素. 分析测试学报, 2011, 30(1): 6-12.
Gong X M, Ren Y P, Dong J, Sun J, Li J, Jin C, Yu J L. Determination of 18 mycotoxin contaminants in peanuts and oils by gel permeation chromatography and ultra performance liquid chromatography-tandem mass spectrometry. Journal of Instrumental Analysis, 2011, 30(1): 6-12. (in Chinese)
[35] Zhang D H, Li P W, Zhang Q, Zhang W, Huang Y L, Ding X X, Jiang J. Production of ultrasensitive generic monoclonal antibodies against major aflatoxins using a modified two-step screening procedure. Analytica Chimica Acta, 2009, 636: 63-69.
[36] Li X, Li P W, Zhang Q, Li Y Y, Zhang W, Ding X X. Molecular characterization of monoclonal antibodies against aflatoxins: a possible explanation for the highest sensitivity. Analytical Chemistry, 2012, 84: 5229-5235.
[37] Li P W, Zhang Q, Zhang W. Immunoassays for aflatoxins. Trends in Analytical Chemistry, 2009, 28(9): 1115-1126.
[38] Wang Y R, Li P W, Majkova Z, Bever C R S, Kim, H J, Zhang Q, Dechant J E, Gee S J, Hammock B D. Isolation of alpaca anti-idiotypic heavy chain single domain antibody for the aflatoxin immunoassay. Analytical Chemistry, 2013, 85: 8298-8303.
[39] Zhang D H, Li P W, Zhang Q, Zhang W. Ultrasensitive nanogold probe-based immunochromatographic assay for simultaneous detection of total aflatoxins in peanuts. Biosensors and Bioelectronics, 2011, 26: 2877-2882.
[40] Li X, Li P W, Zhang Q,Li R,Zhang W,Zhang Z W,Ding X X,Tang X Q. Multi-component immunochromatographic assay for simultaneous detection of aflatoxin B1, ochratoxin A and zearalenone in agro-food. Biosensors and Bioelectronics, 2013, 49: 426-432.
[41] Zhang D H, Li P W, Zhang Q, Li R, Zhang W, Ding X X, Li C M. A naked-eye based strategy for semiquantitative immuno- chromatographic assay. Analytica Chimica Acta, 2012, 740: 74-79.
[42] 李培武, 李冉, 张奇, 丁小霞, 张文, 张兆威. 黄曲霉毒素B1流动滞后免疫时间分荧光速测试剂盒及其应用: 中国, 201310115724. 2013-09-04.
Li P W, Li R, Zhang Q, Ding X X, Zhang W, Zhang Z W. Rapid time-resolved flurensent lateral flow immunoassay kit for aflatoxin B1 and its application: China, 201310115724. 2013-09-04. ( in Chinese)
[43] 李斌, 赵春江, 胡梅. 我国当前农产品产地土壤重金属污染形势及检测技术分析. 农业环境与发展, 2013, 30(5): 1-7.
Li B, Zhao C J, Hu M. Current situation of heavy metals pollution in soil at farmland and detection technologies analysis in China. Agro-Environment and Development, 2013, 30(5): 1-7. (in Chinese)
[44] 姚振兴, 辛晓东, 司维, 赵杰, 陈国栋, 赵伟, 杨健, 杜斌. 重金属检测方法的研究进展. 分析测试技术与仪器, 2011, 17(1): 29-35.
Yao Z X, Xin X D, Si W, Zhao J, Chen G D, Zhao W, Yang J, Du B. Progress in study on detection of heavy metals. Analysis and testing technology and Instruments, 2011, 17(1): 29-35. (in Chinese)
[45] 陈冠宁, 宋志峰, 魏春雁. 重金属检测技术研究进展及其在农产品检测中的应用. 吉林农业科学, 2012, 37(6): 61-64, 71.
Chen G N, Song Z F, Wei C Y. Progress in detection technology of heavy metal and application in the inspection of agricultural products. Journal of Jilin Agricultural Sciences, 2012, 37(6): 61-64, 71. (in Chinese)
[46] Zhu X X, Hu B S, Lou Y, Xu L N, Yang F L, Yu H N, Blake D A, Liu F Q. Characterization of Monoclonal Antibodies for Lead−Chelate Complexes: Applications in Antibody-Based Assays. Journal of Agricultural and Food Chemistry, 2007, 55(13): 4993-4998.
[47] Zhu X X, Xu L N, Lou Y, Yu H N, Li X, Blake D A, Liu F Q. Preparation of specific monoclonal antibodies (MAbs) against heavy metals: MAbs that recognize chelated cadmium ions. Journal of Agricultural and Food Chemistry, 2007, 55(19): 7648-7653.
[48] Lou Y, Yang F, Zhu X, Liu F. Production of a specific monoclonal antibody against mercury-chelate complexes and its application in antibody-based assays. Food and Agricultural Immunology, 2009, 20(1): 23-33.
[49] Zhu X X, Kriegel A M, Boustany C A, Blake D A. Single-Chain Variable Fragment (scFv) Antibodies Optimized for Environmental Analysis of Uranium. Analytical Chemistry, 2011, 83(10): 3717-3724.
[50] Marzo A M L, Pons J, Blake D A, Merkoçi A. All-integrated and highly sensitive paper based device with sample treatment platform for Cd2+ immunodetection in Drinking/Tap Waters. Analytical Chemistry, 2013, 85(7): 3532-3538.
[51] Brandon E F A, Bulder A S, van Engelen J G M, Mahieu C M, Mennes W C, Pronk M E J, Rietveld A G, van de Ven B M, ten Voorde S E C G, Wolterink G, Slib W, Zeilmaker M J, Bessems J G M. Does EU legislation allow the use of the Benchmark dose (BMD) approach for risk assessment. Regulatory Toxicology and Pharmacology, 2013, 67: 182-188.
[52] WHO (World Health Organization). Acute and chronic dietary exposure assessment [EB/OL]. http://www.who.int/foodsafety/chem/ IEDI_calculation14_FAO1.xlt. 2014-2-16.
[53] Keikotlhaile B M, Spanoghe P . Pesticide residues in fruits and vegetables, pesticides-formulations, Effects, Fate[EB/OL]. http://www. intechopen.com/books/pesticides-formulations-effects-fate/pesticide-residues-in-fruits-andvegetables. 2014-2-20.
[54] Chen C, Li Y, Chen M X, Chen Z J, Qian Y Z. Organophosphorus pesticide residues in milled rice on the Chinese market and dietary risk assessment. Food Additives & Contaminants: Part A, 2009, 26(3): 340-347.
[55] Claeys W L, Schmit J, Bragard C, Maghuni-Rogister G, Pussemier L, Schiffers B. Exposure of several Belgian consumer groups to pesticide residues through fresh fruit and vegetable consumption. Food Control, 2011, 22: 508-516.
[56] Lozowicka B, Jankowska M, Kaczynski P. Pesticide residues in Brassica vegetables and exposure assessment of consumers. Food Control, 2012, 25: 561-575.
[57] Nougadere A, Sirot V. Kadar A, Fastier A, Truchot E, Vergnet C, Hommet F, Bayle J, Gros P, Leblanc J. Total dietary study on pesticide residues in France: levels in food as consumed and chronic dietary risk to consumers. Environment International, 2012, 45: 135-150.
[58] 张志恒, 汤涛, 徐浩, 李振, 杨桂林, 王强. 果蔬中氯吡脲残留的膳食摄入风险评估. 中国农业科学,2012, 45(10): 1982-1991.
Zhang Z H, Tang T, Xu H, Li Z, Yang G L, Wang Q. Dietary intake risk assessment of forchlorfenuron residue in fruits and vegetables. Scientia Agriculturae Sinica, 2012, 45(8): 1982-1991. (in Chinese)
[59] Reffstrup T K, Larsen J C, Meyer O. Risk assessment of mixtures of pesticides. Current approaches and future strategies. Regulatory Toxicology and Phamacology, 2010, 56(2): 174-192.
[60] 李耘, 钱永忠, 陈晨, 陈志军, 张星联, 许彦阳, 孙秀兰. 混合化学物风险评估技术及研究进展. 农产品质量与安全, 2013, 6: 42-48.
Li Y, Qian Y Z, Chen C, Chen Z J, Zhang X L, Xu Y Y, Sun X L. Recent advances in risk assessment of mixtures of Chemical. Agricultural Quality and Standards, 2013, 6: 42-48. (in Chinese)
[61] Bobbis A R, Ossendorp B C, Banasiak U, Hamey P Y, Sebestyen I, Moretto A. Cumulative risk assessment of pesticide residues in food. Toxicology Letters, 2008, 180: 137-150.
[62] 陈晨, 李耘, 陈志军, 钱永忠. 美国EPA农药残留急性膳食暴露评估方法. 中国农学通报, 2009, 25(16): 69-74.
Chen C, Li Y, Chen Z J, Qian Y Z, US EPA acute dietary exposure assessment method for pesticide residue in food. Chinese Agricultural Science Bulletin, 2009, 25(16): 69-74. (in Chinese)
[63] 钱永忠, 陈晨, 李耘. 美国应用于人体暴露评估模型原理和方法. 农业质量标准, 2008, 4: 45-48.
Qian Y Z, Chen C, Li Y. Principle and approach of human exposure assessment in USA. Agricultural Quality and Standards, 2008, 4: 45-48. (in Chinese)
[64] United states Environment Protection Agency. Assessing health risks from pesticides [EB/OL].http://www.epa.gov/pesticides/factsheets/ riskassess.htm. 2014-2-20.
[65] Bosgra S, van der Voet H, Boon P E, Slob W. An integrated probabilistic framework for cumulative risk assessment of common mechanism chemicals in food: an example with organophosphorus pesticides. Regulatory Toxicology and Pharmacology, 2009, 54: 124-133.
[66] 李培武, 丁小霞, 白艺珍, 周海燕, 印南日. 农产品黄曲霉毒素风险评估研究进展. 中国农业科学, 2013, 46(12): 2534-2542.
Li P W, Ding X X, Bai Y Z, Zhou H Y, Yin N R. Advance in Research on Risk Assessment of Aflatoxin in Agricultural Products. Scientia Agricultura Sinica, 2013, 46(12): 2534-3542. (in Chinese)
[67] Cano-Sancho G, Ramos A J, Marin S, Sanchis V. Fumonisins in Catalonia(Spain) and exposure assessment of special population groups. Food Additives and Contaminants, 2012, 29(5): 799-808.
[68] Sugita-Konishi Y, Sato T, Saito S, Nakajima M, Tabata S, Tanaka T, Norizuki H, Itoh Y, Kai S, Sugiyama K, Kamata Y, Yoshiike N, Kumagai S. Exposure to aflatoxins in Japan: risk assessment for aflatoxin B1. Food Additives and Contaminants, 2010, 27(3): 365-372.
[69] Signorin M L, Gaggiotti M, Molineri A, Chiericatti C A, Zapata de Basilico M L, Badilico J C, Pisani M. Exposure assessment of mycotoxins in cow’s milk in Argentina. Food and Chemical Toxicology, 2012, 50: 250-257.
[70] Ding X X, Li P P, Bai Y Z, Zhou H Y. Aflatoxin B1 in post-harvest peanuts and dietary risk in China. Food Control, 2012, 23(1): 143-148.
[71] Zhao X B, Schaffner D W, Yue T L. Quantification of aflatioxin risk associated with Chinese spices: Point and probability risk assessments for aflatoxin B1. Food Control, 2013, 33: 366-377.
[72] 郭耀东, 刘艺茹, 袁亚宏, 岳田利. 我国主要食品中伏马毒素污染水平分析与风险评估. 西北农林科技大学学报, 2014, 42(1): 1-5.
Guo Y D, Liu Y R, Yuan Y H, Yue T L. Fumonisins contamination in major food products and its dietary risk assessment in China. Journal of Northwest A&F University, 2014, 42(1): 1-5. (in Chinese)
[73] Cano-Sancho G, Marin S, Ramos A J, Sanchis V. Occurrence of zearalenone, an oestrogenic mycotoxin, in Catalonia (Spain) and exposure assessment. Food and Chemical Toxicology, 2012, 50: 835-839.
[74] Zheng H, Nie D X, Yang X L, Wang J H, Peng S J, Wu A B. Quantitative assessment of risk associated with dietary intake of mycotoxin ochratoxin A on the adult inhabitants in Shanghai city of P.R.China. Food Control, 2013, 32: 490-495.
[75] Serrano A B, Font G, Ruiz M J, Ferrer E. Co-occurrence and risk assessment of mycotoxins in food and diet from Mediterranean area. Food Chemistry, 2012, 135: 423-429.
[76] Wu F, Stacy S L, Kensler T W. Global Risk Assessment of Aflatoxins in Maize and Peanuts: Are Regulatory Standards Adequately Protective. Toxicological Sciences, 2013, 135(1): 251-259.
[77] Lee H M, Hwang J H, Ryuem T K, Jang D D, Yang J H. Risk assessment of aflatoxin B1 from food consumption in the Korean general population. Human and Ecological Risk Assessment, 2009, 15: 1273-1285.
[78] 丁小霞, 李培武, 周海燕, 白艺珍, 印南日. 黄曲霉毒素限量标准对我国居民消费安全和花生产业的影响. 中国油料作物学报, 2011, 33(2): 180-184.
Ding X X, Li P W, Zhou H Y, Bai Y Z, Yin N R. Effect of aflatoxin maximum limit standards on consumer dietary risk and peanut industry in China. Chinese Journal of Oil Crops Sciences, 2011, 33(2): 180-184.(in Chinese)
[79] Chauhan Y S, Wright G C, Rachaputi R C N, Holzworth D, Broome A, Krosch S, Robertson M J. Application of a model to assess aflatoxin risk in peanut. Journal of Agricultural Science, 2010, 148: 341-351.
[80] Noor-ul-Amin, Hussain A, Alamzeb S, Begum S. Accumulation of heavy metals in edible parts of vegetables irrigated with waste water and their dairy intake to adults and children, District Masdan, Pakistan. Food Chemistry, 2013, 136: 1515-1523.
[81] Mahmood A, Malik R N. Human health risk assessment of heavy metals via consumption of contaminated vegetables collected from different irrigation sources in Lahore, Pakistan. Arabian. Journal of Chemistry, 2014, 7: 91-99.
[82] Singh A, Sharma R K, Agrawal M, Marshall F M. Health risk assessment of heavy metals via dietary intake of foodstuffs from the wastewater irrigated site of a dry tropical area of India. Food and Chemical Toxicology, 2010, 48: 611-619.
[83] Li Q S, Chen Y, Fu H B, Cui Z H, Shi L, Wang L L, Liu Z F. Health risk of heavy metals in food crops grown on reclaimed tidal flat soil in the Pearl River Eatuary, China. Journal of Hazardous Materials, 2012, 227-228: 148-154.
[84] 李如忠, 潘成荣, 徐晶晶, 陈婧, 姜艳敏. 典型有色金属矿业城市零星菜地蔬菜重金属污染及健康风险评估. 环境科学, 2013, 34(3): 1076-1085.
Li R Z, Pan C R, Xu J J, Chen J, Jiang Y M. Contamination and health risk for heavy metals via consumption of vegetables grown in fragmentary vegetable plots from a typical nonferrous metals mine city. Environmental Science, 2013, 34(3): 1076-1085. (in Chinese)
[85] Harmanescu M, Alda L M, Bordean D M, Gogoasa L, Gergen L. Heavy metals health risk assessment for population via consumption of vegetables grown in old mining area; a case study: Banat County, Romania. Chemistry Central Journal, 2011, 5: 64-73.
[86] Khan K, Lu Y L, Khan H, Ishtiaq M, Khan S, Waqas M, Wei L, Wang T Y. Heavy metals in agricultural soils and crops and their health risks in Swat District, northern Parkistan. Food and Chemical Toxicology, 2013, 58: 449-458.
[87] 沈群超, 胡寅侠, 蒋开杰, 翁颖. 慈溪地产大米重金属调查及其健康风险评估.中国稻米, 2013, 19(3): 79-81.
Shen Q C, Hu Y X, Jiang K J, Weng Y. Investigation and health risk assessment of heavy metals of rice is Ci XI. Chinese Rice, 2013, 19(3): 79-81. (in Chinese)
[88] 任金萍. 平凉市地产蔬菜重金属污染检测及其对人体健康风险评估. 甘肃农业大学学报, 2013, 48(5): 126-129.
Ren J P. Detection of heavy metal pollution of vegetables in Pingliang and assessment of human healthy risk. Journal of Gansu Agricultural University, 2013, 48(5): 126-129. (in Chinese)
[89] 张徐惠群, 杨暄, 马丽艳, 戴蕴青. 香菇和平菇中几种重金属元素的质量分数及其健康风险评估. 中国农业大学学报, 2013, 18(3): 163-171.
Zhang X H Q, Yang X, Ma L Y, Dai Y Q. Health risk evaluation on heavy metal concentrations in Lentionusedodes and Pleurotusostreatus. Journal of China Agricultural University, 2013, 18(3): 163-171. (in Chinese)
[90] 钱永忠, 王芳, 高山, 高观. 新时期农产品质量安全监管问题探讨. 农产品质量安全, 2010, 1: 13-15.
Qian Y Z, Wang F, Gao S, Gao G. Study on quality and safety regulatory issues of agricultural products in the new era. Quality and Safety of Agricultural Products, 2010, 1: 13-15. (in Chinese)
[91] 罗小勇. 农药残留及对策. 中国农学通报, 2009, 25(18): 344-347.
Luo X Y. Residues from pesticides and countermeasures. Chinese Agricultural Science Bulletin, 2009, 25(18): 344-347. (in Chinese)
[92] 程志明, 黄佩娜, 林蔚, 蔡建成, 林木成. 水果产品农药残留的防控技术探讨. 福建热作科技, 2006, 31(1): 12-13.
Chen Z M, Huang P N, Lin W, Cai J C, Lin M C. Study on prevention and control technology of pesticide residues in fruit products. Fujian Science & Technology of Tropical Crops, 2006, 31(1): 12-13. (in Chinese)
[93] 王彬, 赵家良. 蔬菜农药残留现状及防控对策. 云南农业, 2009(7): 36.
Wang B, Zhao J L. Status of pesticide residues and prevention countermeasures in vegetables. Yunnan Agriculture, 2009(7): 36. (in Chinese)
[94] 王隆都, 谢锦秀. 茶叶农药残留原因与防控技术. 福建农业, 2010(4): 35.
Wang L D, Xie J R. The reason of pesticide residues in tea and prevention countermeasures. Fujian Agriculture, 2010(4): 35. (in Chinese)
[95] 李莉, 江树人, 刘丰茂. 蔬菜中农药残留去除方法. 农药, 2005, 44: 347-351.
Li L, Jiang S R, Liu F M. Methods for removing pesticide residues from vegetables. Chinese Journal of Pesticides, 2005, 44: 347-351. (in Chinese)
[96] MargaritaStoytcheva. Biodegradation of Pesticides, in “Pesticides in the Modern World-Pesticides Use and Management”. Croatia, InTech, 2011.
[97] Fenner K, Canonica S, Wackett L P, Elsner M. Evaluating pesticide degradation in the environment: blind spots and emerging opportunities. Science, 2013, 341: 752-758.
[98] Edlayne G, Simone A, Felicio J D. Chemical and biological approaches for mycotoxin control: a review. Recent Patents on Food, Nutrition and Agriculture, 2009, 1(2): 155-161.
[99] 孙长坡, 代岩石, 王松雪, 张艳. 利用生物技术防控、消减粮食及其制品中的真菌毒素. 中国粮油学报, 2009, 24(11): 97-101.
Sun C P, Dai Y S, Wang S X, Zhang Y. Preventing and detoxifying mycotoxin in grains and their products by biotechnology. Journal of the Chinese Cereals and Oils Association, 2009, 24(11): 97-101. (in Chinese)
[100] 姜慧芳, 任小平, 王圣玉, 张晓杰, 黄家权, 廖伯寿. 利用核心种质发掘及评价花生抗黄曲霉资源. 作物学报, 2010, 36(3): 428-434.
Jiang H F, Ren X P, Wang S Y, Zhang X J, Huang J Q, Liao B S. Development and evaluation of peanut germplasm with resistance to aspergillus flavus from core collection. Acta Agronomica Sinica, 2010, 36(3): 428-434. (in Chinese)
[101] 田立荣, 廖伯寿, 李玉荣, 程增书, 陈四龙, 王瑾, 周立杰. 花生抗黄曲霉遗传改良研究进展. 河北农业科学, 2010, 14: 80-83.
Tian L Y, Liao B Q, Li Y R, Chen Z S, Chen S L, Wang J, Zhou L J. Progress in genetic improvement of resistance to ailatoxin in peanut. Journal of Hebei Agricultural Sciences, 2010, 14: 80-83. (in Chinese)
[102] 王后苗, 黄家权, 雷永, 晏立英, 王圣玉, 姜慧芳, 任小平, 娄庆任, 廖伯寿. 花生种子白藜芦醇含量与黄曲霉产毒抗性的关系. 作物学报, 2012, 38(10): 1875-1883.
Wang H M, Huang J Q, Lei Y, Yan L Y, Wang S Y, Jiang H F, Ren X P, Lou Q R, Liao B Q. Relationship of resveratrol content and resistance to aflatoxin accumulation caused by Aspergillus flavus in peanut seeds. Acta Agronomica Sinica, 2012, 38(10): 1875-1883. (in Chinese)
[103] 张道宏, 李培武, 张奇, 张文, 丁小霞, 王秀嫔, 姜俊. 污染粮油食品的主要真菌毒素及胶体金免疫层析技术在快速检测中的应用. 中国油料作物学报, 2010, 32(4): 577-582.
Zhang D H, Li P W, Zhang Q, Zhang W, Ding X X, Wang X P, Jiang J. Major mycotoxins contaminating the cereal and oil foods and application of colloidal-gold immunochromatography assay in mycotoxins detection. Chinese Journal of Oil Crop Sciences, 2010, 32(4): 577-582. (in Chinese)
[104] Li X, Zhang Z W, Li P W, Zhang Q, Zhang W, Ding X X. Determination for major chemicalcontaminants in tea (Camellia sinensis) matrices: a review. Food Research International, 2013, 53(2): 649-658.
[105] Kensler T W, Roebuck B D, Wogan G N, Groopman J D. Aflatoxin: a 50-year odyssey of mechanistic and translational toxicology. Toxicological Sciences, 2011, 120(S1): S28-S48.
[106] 王后苗, 廖伯寿. 农作物收获前黄曲霉毒素污染与控制措施. 作物学报, 2012, 38(1): 1-9.
Wang H, Liao B Q. Preharvest Aflatoxin contamination in crops and its management. Acta Agronomica Sinica, 2012, 38(1): 1-9. (in Chinese)
[107] 李培武, 张道宏, 杨扬, 崔野韩, 张奇, 张文, 丁小霞, 王秀嫔, 姜俊. 粮油制品中黄曲霉毒素脱毒研究进展. 中国油料作物学报, 2010, 32(2): 315-319.
Li P W, Zhang D H, Yang Y, Cui Y H, Zhang Q, Zhang W, Ding X X, Wang X P, Jiang J. A review of the detoxification of aflatoxins in cereal and oilseeds products. Chinese Journal of Oil Crop Sciences, 2010, 32(2): 315-319. (in Chinese)
[108] 毛雪飞, 汤晓艳, 王艳, 王敏. 从“镉大米”事件谈我国种植业农产品重金属污染的来源与防控对策. 农产品质量与安全, 2013, 4: 57-73.
Mao X F, Tang X Y, Wang Y, Wang M. Study on the origin and prevention and control countermeasure of heavy metals pollution in cultivation industry of agricultural products of our country from the "Cadmium rice" event. Quality and Safety of Agricultural Products, 2013, 4: 57-73. (in Chinese)
[109] Cheng S. Heavy metal pollution in China: origin, pattern and control. Environmental Science and Pollution Research, 2003, 10(3): 192-198.
[110] 王其枫, 王富华, 孙芳芳, 王旭, 文典, 刘香香, 赵凯. 广东韶关主要矿区周边农田土壤铅、镉的形态分布及生物有效性研究. 农业环境科学学报, 2012, 31(6): 1097-1103.
Wang Q F, Wang F H, Sun F F, Wang X, Wen D, Liu X X, Zhao K. Fractionation and bioavailability of Pb and Cd in agricultural soils around mining area in Shaoguan Guangdong province, China. Journal of Agro-Environment Science, 2012, 31(6): 1097-1103. (in Chinese)
[111] 郑顺安, 陈春, 郑向群, 梁军锋, 刘潇威, 沈跃, 李松. 污染土壤不同粒级团聚体中铅的富集特征及其与叶类蔬菜铅吸收之间的相关性. 农业环境科学学报, 2013, 32(3): 556-564.
Zheng S A, Chen C, Zhen X Q, Liang J F, Liao X W, Shen Y, Li S. A correlation between enrichment characteristics of lead in different particle-size fractions of soil aggregates and lead concentrations of leafy vegetables. Journal of Agro-Environment Science, 2013, 32(3): 556-564. (in Chinese)
[112] Kobayashi N I,Tanoi K, Hirose A, Nakanishi T M. Characterization of rapid intervascular transport of cadmium in rice stem by radioisotope imaging. Journal of Experimental Botany, 2013, 64(2): 507-517.
[113] 朱智伟. 农产品重金属污染与安全性. 北京工商大学学报: 自然科学版, 2012, 30(5): 16-25.
Zhu Z W. Heavy metal contamination in agricultural products and food safety. Journal of Beijing Technology and Business University: Natural Science Edition, 2012, 30(5): 16-25. (in Chinese)
[114] Wu L H, Luo Y M, Christie P, Wong M H. Effects of EDTA and low molecular weight organic acids on soil solution properties of a heavy metal polluted soil. Chemosphere, 2003, 50(6): 819-822.
[115] Erdem E, Karapinar N, Donat R. The removal of heavy metal cations by natural zeolites. Journal of Colloid and Interface Science, 2004, 280(2): 309-314.
[116] 查燕, 杨居荣, 刘虹, 何孟常. 污染谷物中重金属的分布及加工过程的影响. 环境科学, 2000, 3: 52-55.
Zha Y, Yang J R, Liu H, He M C. Distribution of heavy metals in polluted crops seeds and the effect of heavy metals in the food processing. Environmental Science, 2000, 3: 52-55. (in Chinese)
[117] 孟橘, 倪芳妍, 杨帆, 张磊, 田淑梅. 油脂加工过程中重金属危害的研究. 中国油脂, 2008, 33(1): 16-19.
Meng J, Ni F Y, Yang F, Zhang L, Tian S M. Harm of heavy metal in the course of oil processing. China Oils And Fats, 2008, 33(1): 16-19. (in Chinese)
[118] 高秀兵, 段学艺, 张宝林, 陈娟, 何莲, 胡华健. 绿茶加工过程中农药残留、重金属含量及微生物数量的变化. 西南农业学报, 2012, 25(3): 958-961.
Gao X B, Duan X Y, Zhang B L, Chen J, He L, Hu H J. Changing of pesticide residue, heavy metal contents and microorganism quantity in processing of green tea. Southwest China Journal of Agricultural Sciences, 2012, 25(3): 958-961. (in Chinese)
[119] Demirbas A. Heavy metal adsorption onto agro-based waste materials: a review. Journal of Hazardous Materials, 2008, 157: 220-229.
[120] 金发忠. 我国农产品质量安全风险评估的体系构建及运行管理. 农产品质量与安全, 2014(3): 3-11.
Jin F Z. Building and operation management of agro-product quality and safety risk assessment system in China. Quality and Safety of agro-products, 2014(3): 3-11. (in Chinese) |
[1] | 王一丹,杨发龙,陈弟诗,向华,任玉鹏. 猪腹泻病毒一步法多重TaqMan荧光定量RT-PCR检测法的建立及应用[J]. 中国农业科学, 2023, 56(1): 179-192. |
[2] | 李志玲,李香菊,崔海兰,于海燕,陈景超. 牛筋草EPSPS酶联免疫试剂盒的研发及应用[J]. 中国农业科学, 2022, 55(24): 4851-4862. |
[3] | 谢丽雪,张小艳,张立杰,郑姗,李韬. 侵染西番莲的东亚西番莲病毒全基因组序列特征及TC-RT-PCR检测技术[J]. 中国农业科学, 2022, 55(22): 4408-4418. |
[4] | 崔江宽,任豪豪,曹梦园,陈昆圆,周博,蒋士君,汤继华. 玉米孢囊线虫SCAR-PCR快速分子检测技术[J]. 中国农业科学, 2022, 55(17): 3334-3342. |
[5] | 张静远,缪发明,陈腾,李敏,扈荣良. 非洲猪瘟实时荧光RPA诊断方法建立及应用[J]. 中国农业科学, 2022, 55(1): 197-207. |
[6] | 李镇希,李文婷,黄家权,郑正,许美容,邓晓玲. 膜吸附法结合可视化环介导等温扩增技术检测柑橘黄龙病菌[J]. 中国农业科学, 2022, 55(1): 74-84. |
[7] | 段玉,许建建,马志敏,宾羽,周常勇,宋震. 柑橘叶斑驳病毒的逆转录重组酶聚合酶扩增检测[J]. 中国农业科学, 2021, 54(9): 1904-1912. |
[8] | 白雪,惠腾,王振宇,曹云刚,张德权. 高效液相色谱-荧光检测法检测烤肉制品中5种硝基多环芳烃[J]. 中国农业科学, 2021, 54(5): 1055-1062. |
[9] | 李佳佳,洪慧龙,万明月,储丽,赵敬会,汪明华,徐志鹏,张阴,黄志平,张文明,王晓波,邱丽娟. 基于近红外光谱的大豆茎秆化学组分含量检测模型构建与应用[J]. 中国农业科学, 2021, 54(5): 887-900. |
[10] | 张峭,王克. 农业生产风险评估及农业保险费率厘定的不确定性:研究进展和破解之道[J]. 中国农业科学, 2021, 54(22): 4778-4786. |
[11] | 马志敏,许建建,段玉,王春庆,苏越,张琦,宾羽,周常勇,宋震. 柑橘黄化脉明病毒RT-RPA检测方法的建立[J]. 中国农业科学, 2021, 54(15): 3241-3249. |
[12] | 陈鹏飞,马啸. 作物种植行自动检测研究现状与趋势[J]. 中国农业科学, 2021, 54(13): 2737-2745. |
[13] | 惠媛媛,彭海帅,王毕妮,张富新,刘玉芳,贾蓉,任荣. 基于适配体的光学和电化学法对食源性致病菌检测的研究进展[J]. 中国农业科学, 2021, 54(11): 2419-2433. |
[14] | 张清安,陈博宇. 葡萄酒中与风味相关4类含硫化合物的研究进展[J]. 中国农业科学, 2020, 53(5): 1029-1045. |
[15] | 徐默然,蔺瑞明,王凤涛,冯晶,徐世昌. 103份小麦品种(系)抗条锈性和遗传多样性评价及基因检测[J]. 中国农业科学, 2020, 53(4): 748-760. |
|