密花胡颓子果实主要类胡萝卜素组分及含量分析

doi:10.3864/j.issn.0578-1752.2014.08.021

摘要/Abstract

摘要： 【目的】胡颓子属植物牛奶子果实大量积累番茄红素，高达番茄的17倍，而该属中果实大、口感好的密花胡颓子果实中主要类胡萝卜素组分及含量尚不清楚。研究利用高效液相色谱法检测德甜密花胡颓子1号和2号2个品种的成熟果实中主要类胡萝卜素组分及含量。【方法】以甲醇∶氯仿=1∶2（v/v）提取果实中类胡萝卜素，高效液相色谱法（Agilent 1260）进行类胡萝卜素定性定量分析。色谱条件：YMC C30分析色谱柱（4.6 mm×250 mm，5 μm）；流动相为A（100％甲醇）、B（含0.2％乙酸铵的80％甲醇）和C（甲基叔丁基醚），采用梯度洗脱，洗脱程序为0—6 min由95％A+5％B组成，7 min时梯度改变为80％A+5％B+15％C，12—32 min梯度改变为30％A+5％B+65％C，48—50 min梯度改变为95％A+5％B，维持该浓度至分析结束（60 min），流速为1 mL•min-1，柱温25℃，进样量20 μL，检测波长450 nm。【结果】德甜密花胡颓子1号和2号2个品种的成熟果实均积累了大量的番茄红素和β-胡萝卜素，品种间的积累量存在差异。其中德甜密花胡颓子1号果实中β-胡萝卜素积累量达（231.84±16.00）µg•g-1DW，是目前已报道植物中β-胡萝卜素含量高的物种之一；德甜密花胡颓子2号品种果实中的番茄红素含量高达（1 054.10±29.19）µg•g-1DW，是番茄果实（150 µg•g-1DW）的7倍。【结论】密花胡颓子果实中高积累番茄红素和β-胡萝卜素，是继牛奶子之后，在胡颓子属中发现的又一高积累特异类胡萝卜素的野生植物资源，为今后研究果实高积累番茄红素和β-胡萝卜素的分子机理提供了理想的研究材料。

关键词: 密花胡颓子 , 番茄红素 , &beta, -胡萝卜素 , 高效液相色谱

Abstract: 【Objective】The berry of Elaeagnus umbellata Thunb. was found to accumulate lycopene up to 54 mg per 100 g of fresh weight, which is about 17 times higher than that of tomato. However, little is known about the carotenoid composition and content in the big and good tasty flesh of E. conferta Roxb., also a member of Elaeagnaceae family. This study aimed to analyze carotenoid components and contents in the fruit of E. conferta Roxb. by high performance liquid chromatograph（HPLC）method.【Method】 Methanol:chloroform (1:2 v/v) was used to extract carotenoids from ripe fruits of E. conferta Roxb.. The separation was carried on a YMC C30 (4.6 mm×250 mm, 5 µm, column temperature 235℃) and an injection volume was 20 μL. Methanol (A), 80% methanol containing 0.2% ammonium acetate (B) and methyl tert-butyl ether (C) were used as the mobile phase at a flow rate of 1.0 mL•min-1 through gradient elution: (1) 0-6 min, 95%A+5%B; (2) 7-11 min, 80%A+5%B+15%C; (3) 12-32 min, 30%A+ 5%B+65%C; (4) 33 min to end, 95%A+5%B. The eluent was detected at 244 nm. 【Result】 Lycopene and β-carotene were two major carotenoids in the flesh of E. conferta Roxb., and their content differed markedly between two genetic genotypes. The β-carotene content in Detianmihuahutuizi 1 was up to (231.84±16.00) µg•g-1DW, which is one of the highest ones in the known species. The flesh of Detianmihuahutuizi 2 contained lycopene of (1 054.10±29.19) µg•g-1DW, which is about seven times higher than that of tomato.【Conclusion】The results showed that the flesh of E. conferta Roxb. accumulated abundant lycopene and β-carotene, which endow E. conferta Roxb. as a wilding fruit resource with great development and application value. Meanwhile, E. conferta Roxb. also is an ideal research material for studying molecular mechanism of high accumulation of lycopene and β-carotene in fruit.

Key words: Elaeagnus conferta Roxb , lycopene , β-carotene , high performance liquid chromatography

胡海涛1, 金晓琴1, 成龙平1, 杨莉1, 陈建华1, 番汝昌2, 杨玲1. 密花胡颓子果实主要类胡萝卜素组分及含量分析[J]. 中国农业科学, 2014, 47(8): 1652-1656.

HU Hai-Tao-1, JIN Xiao-Qin-1, CHENG Long-Ping-1, YANG Li-1, CHEN Jian-Hua-1, FAN Ru-Chang-2, YANG Ling-1. Determination of Carotenoids in the Fruit of Elaeagnus conferta Roxb. by High Performance Liquid Chromatography[J]. Scientia Agricultura Sinica, 2014, 47(8): 1652-1656.

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