河北邢台和江苏南京地区棉蚜对丁硫克百威和吡虫啉的抗性

doi:10.3864/j.issn.0578-1752.2013.16.008

摘要/Abstract

摘要： 【目的】对河北邢台和江苏南京地区棉蚜（Aphis gossypii Glover）进行抗性监测，了解棉蚜对丁硫克百威和吡虫啉的抗性发展情况，为抗性治理提供理论依据。【方法】以用药水平最低的新疆南疆种群作为相对敏感种群，通过室内毒力测定、离体酶活力测定、相对表达量分析，研究新疆南疆、河北邢台及江苏南京3个地区棉蚜对丁硫克百威和吡虫啉潜在的代谢抗性机制。【结果】河北邢台和江苏南京地区的棉蚜对丁硫克百威仍处于敏感阶段；但对吡虫啉的抗性已有所发展，抗性水平分别为4.87和8.72倍，为低水平抗性阶段。解毒代谢酶活力对比研究发现，3个地区田间种群之间多功能氧化酶、酯酶及谷胱甘肽转移酶的活力均存在显著差异，江苏南京种群的酯酶活力显著高于新疆南疆种群。定量PCR分析表明，河北邢台地区棉蚜的羧酸酯酶表达量较高，是新疆种群棉蚜的3.28倍。【结论】田间棉蚜已对吡虫啉产生了低水平的抗性，酯酶活力升高在棉蚜对吡虫啉低水平抗性形成中发挥作用。

关键词: 棉蚜 , 丁硫克百威 , 吡虫啉 , 抗性

Abstract: 【Objective】 The objective of this study is to monitor the insecticide resistance of Aphis gossypii Glover in filed to carbosulfan and imidacloprid, and to provide a better understanding of resistance mechanism. 【Method】The cotton aphids were collected from fields in Nanjiang city of Xinjiang Uygur Autonomous Region (XJ), Xingtai city of Hebei Province (HB), and Nanjing city of Jiangsu Province (JS). The resistances of field cotton aphid to conventionally used insecticides imidacloprid and carbosulfan were investigated. The cotton aphids from Xinjiang were treated as the sensitive strain. The bioassay, enzyme activities and mRNA relative expression levels by qPCR were performed to study the resistance ratios and their potential molecular mechanisms. 【Result】A. gossypii of Xingtai city of Hebei Province (HB) and Nanjing city of Jiangsu Province (JS) were still sensitive to carbosulfan, but their insecticide resistance to imidacloprid reached 4.87 and 8.72 folds. Three field populations of cotton aphids had significantly different activities of oxidases, esterase and glutathione-S-transferase. The activities of esterase in Nanjing city of Jiangsu Province were the highest among three field populations. Compared with XJ strain, quantitative real-time PCR indicated that the relative expression level of carboxylesterase gene was the highest, which was 3.28-fold higher. 【Conclusion】The cotton aphid has developed low-level resistance to imidacloprid. The increased esterase activity confers the low-level resistance to imidacloprid.

Key words: Aphis gossypii Glover , carbosulfan , imidacloprid , insecticide resistance

王娜, 郎志飞, 贺康, 李飞. 河北邢台和江苏南京地区棉蚜对丁硫克百威和吡虫啉的抗性[J]. 中国农业科学, 2013, 46(16): 3377-3383.

WANG Na, LANG Zhi-Fei, HE Kang, LI Fei. Resistance of Field Population Aphis gossypii Glover to Carbosulfan and Imidacloprid in Xingtai and Nanjing District[J]. Scientia Agricultura Sinica, 2013, 46(16): 3377-3383.

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