影响禽致病性大肠杆菌信号分子AI-2产生的因素分析

doi:10.3864/j.issn.0578-1752.2013.15.017

中国农业科学 ›› 2013, Vol. 46 ›› Issue (15): 3220-3226.doi: 10.3864/j.issn.0578-1752.2013.15.017

• 畜牧·兽医·资源昆虫 • 上一篇下一篇

影响禽致病性大肠杆菌信号分子AI-2产生的因素分析

白灏1, 2, 韩先干1, 刘蕾1, 祁克宗2, 刘海文1, 丁铲1, 于圣青1

1.中国农业科学院上海兽医研究所，上海 200241
2.安徽农业大学动物科技学院，合肥 230036

收稿日期:2012-12-17 出版日期:2013-08-01 发布日期:2013-06-08
通讯作者: 通信作者于圣青，Tel：021-34293461；E-mail：yus@shvri.ac.cn；通信作者韩先干，Tel：021-34293412；E-mail：hanxgan@163.com
作者简介:白灏，Tel：021-34293153；E-mail：baihao07@163.com
基金资助:
国家自然科学基金（31001078，31072161，30871851）

Analysis of Factors Affecting the Activity of AI-2 of Avian Pathogenic Escherichia coli

BAI Hao-1, 2 , HAN Xian-Gan-1, LIU Lei-1, QI Ke-Zong-2, LIU Hai-Wen-1, DING Chan-1, YU Sheng-Qing-1

1.Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241
2.College of Animal Science, Anhui Agricultural University, Hefei 230036

Received:2012-12-17 Online:2013-08-01 Published:2013-06-08

摘要/Abstract

摘要： 【目的】探讨禽致病性大肠杆菌(Avian Pathogenic Escherichia coli, APEC)在不同生长时期和不同培养条件下，信号分子AI-2(Autoinducer-2, AI-2)的合成与luxS和pfs的转录水平之间的关系。【方法】利用哈维弧菌BB170（vibrio harveyi BB170）检测不同生长时期和不同培养条件下APEC的AI-2活性。同时利用Real-time PCR检测APEC不同生长时期和不同培养条件下luxS和pfs的转录水平。【结果】APEC在不同生长时期的AI-2活性与luxS的转录水平保持一致。培养基中加入葡萄糖，麦芽糖和NaCl后，AI-2活性和luxS转录水平都上调，加入蔗糖后则都下调，两者保持一致性。AI-2活性与pfs的转录水平则并不一致。【结论】APEC的AI-2产生水平与luxS的转录水平有高度的相关性，而与pfs的转录水平没有显著相关性；葡萄糖、麦芽糖和NaCl，促进AI -2的合成。

关键词: 禽致病性大肠杆菌 , 信号分子AI-2 , luxS , pfs , Real-time PCR

Abstract: 【Objective】 In this study, the relationship between the transcription level of luxS and pfs and AI-2 production in different growth phases and culture conditions were analyzed. 【Method】 AI-2 activity in different growth phases and culture conditions was measured using the V. harveyi bioluminescence assay.The levels of luxS and pfs mRNA were analyzed using real-time PCR. 【Result】 The results showed that the level of AI-2 was consistent with the level of luxS mRNA in different growth phases. AI-2 production and luxS mRNA by APEC was increased by supplementation of glucose, maltose, and NaCl, while the addition of sucrose was decreased. The transcription level of luxS was correlated to the level of AI-2 production, while the transcription level of pfs did not correlate with the level of AI-2 production. 【Conclusion】 The level of transcription of luxS is highly correlated with the level of AI-2 production,while the level of transcription of pfs does not correlate with the level of AI-2 production. Glucose, maltose and NaCl accelerate the synthesis of AI-2.

Key words: avian pathogenic Escherichia coli , autoindure-2 , luxS , pfs , real-time PCR

白灏1, 2, 韩先干1, 刘蕾1, 祁克宗2, 刘海文1, 丁铲1, 于圣青1. 影响禽致病性大肠杆菌信号分子AI-2产生的因素分析[J]. 中国农业科学, 2013, 46(15): 3220-3226.

BAI Hao-1, 2 , HAN Xian-Gan-1, LIU Lei-1, QI Ke-Zong-2, LIU Hai-Wen-1, DING Chan-1, YU Sheng-Qing-1. Analysis of Factors Affecting the Activity of AI-2 of Avian Pathogenic Escherichia coli[J]. Scientia Agricultura Sinica, 2013, 46(15): 3220-3226.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2013.15.017

https://www.chinaagrisci.com/CN/Y2013/V46/I15/3220

参考文献

[1]Schouler C, Schaeffer B, Brée A, Mora A, Dahbi G, Biet F, Oswalde E, Mainil J, Blanco J, Schouleur M M. Diagnostic strategy for identifying avian pathogenic Escherichia coli based on four patterns of virulence genes. Journal of Clinical Microbiology, 2012, 50(5): 1673-1678.

[2]Tuntufye H N, Lebeer S, Gwakisa P S, Goddeeris B M. Identification of avian pathogenic Escherichia coli genes that are induced in vivo during infection in chickens. Applied and Environmental Microbiology, 2012, 78(9):3343-3351.

[3]Gonzalez J E, Keshavan N D. Messing with bacterial quorum sensing. Microbiology and Molecular Biology Reviews, 2006, 70:859-875.

[4]Han X G, Lu C P. Biological activity and identification of a peptide inhibitor of LuxS from Streptococcus suis serotype 2. FEMS Microbiology Letters, 2009, 294(1):16-23.

[5]Rickard A H, Palmer R J, Blehert D S, Campagna S R, Semmelhack M F, Egland P G, Bassler B L, Kolenbrander P E. Autoinducer 2: aconcentration-dependent signal for mutualistic bacterial biofilm growth. Molecular Microbiology , 2006, 60(6): 1446-1456.

[6]Sperandio V, Mellies J L, Nguyen W, Shin S, Kaper J B. Quorum sensing controls expression of the type III secretion gene transcription and protein secretion in enterohemorrhagic and enteropathogenic Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America, 1999, 96:15196-15201.

[7]Stroeher U H, Paton A W, Ogunniyi A D, Paton J C. Mutation of luxS of Streptococcus pneumoniae affects virulence in a mouse model. Infection and Immunity, 2003, 71:3206-3212.

[8]DeLisa M P, Wu C F, Wang L, Valdes J J, Bentley W E. DNA microarray-based identification of genes controlled by autoinducer 2-stimulated quorum sensing in Escherichia coli. Journal of Bacteriology, 2001, 183: 5239-5247.

[9]Surette M G, Miller M B, Bassler B L. Quorum sensing in Escherichia coli, Salmonella typhimurium, and vibrio harveyi: a new family of genes responsible for autoinducer production. Proceedings of the National Academy of Sciences of the United States of America, 1999, 96:1639-1644.

[10]Pei D H, Zhu J G. Mechanism of action of S-ribosylhomocysteinase (LuxS). Current Opinion in Chemical Biology, 2004, 8(5):492-497.

[11]Schauder S, Shokat K, Surette M G, Bassler B L. The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum-sensing signal molecule. Molecular Microbiology, 2001, 41(2):463-476.

[12]Keersmaecker S C J D, Sonck K, Vanderleyden J. Let LuxS speak up in AI-2 signaling. Trends in Microbiology, 2006, 14(3):114-119.

[13]韩先干, 白灏, 刘蕾, 陈文静, 丁铲, 胡青海, 祁克宗, 于圣青. 禽致病性大肠杆菌安徽分离株luxS和pfs基因的克隆、表达与细胞外合成信号分子AI-2活性检测. 微生物学报，2012, 52(9): 1167-1172.

Han X G, Bai H, Liu L, Chen W J, Ding C, Hu Q H, Qi K Z, Yu S Q. Cloning and expression of luxS and pfs in vitro biosynthesis autoinducer 2 of Avian pathogenic Escherichia coli from Anhui Province. Acta Microbiologica Sinica, 2012, 52(9): 1167-1172. (in Chinese)

[14]Wang Y, Zhang W, Wu Z, Lu C. Reduced virulence is an important characteristic of biofilm infection of Streptococcus suis. FEMS Microbiology Letters, 2011, 316: 36-43.

[15]Surette M G, Bassler B L. Regulation of autoinducer production in Salmonella typhimurium. Molecular Microbiology, 1999, 31(2): 585-595.

[16]Surette M G, Bassler B L. Quorum sensing in Escherichia coli and Salmonella typhimurium. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95(12):7046-7050.

[17]Han X G, Bai Hao, Liu Lei, Dong H, Liu R, Song J, Ding C, Qi K, Liu H, Yu S. The luxS gene functions in the pathogenesis of Avian pathogenic Escherichia coli. Microbial Pathogenesis, 2013, 55: 22-27.

[18]Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2－△△CT method. Methods, 2001, 25: 402-408.

[19]罗哲, 王敏, 杜鸿, 王菲, 孟彦辰, 倪斌, 徐顺高, 黄新祥. LuxS/AI-2对伤寒沙门菌基因表达的调节. 江苏大学学报, 2011, 21(1): 35-41.

Luo Z, Wang M, Du H, Wang F, Meng Y C, Ni B, Xu S G, Huang X X. Analysis of genes expression regulation controlled by luxS/AI-2 in Salmonella enterica serovar Typhi. Journal of Jiangsu University, 2011, 21(1): 35-41.(in Chinese)

[20]Jones M B, Blaser M J. Detection of a luxS-signaling molecule in Bacillus anthracis. Infection and Immunity, 2003, 71: 3914-3919.

[21]Miller M B, Skorupski K, Lenz D H, Taylor R K, Bassler B L. Parallel quorum sensing systems convergeto regulate virulence in vibrio cholera. Cell, 2002,110(3):303-314.

[22]Jenabian S M, Vogensen F K, Jespersen L. The quorum sensing luxS gene is induced in Lactobacillus acidophilus NCFM in response to Listeria monocytogenes. International Journal of Food Microbiology, 2011, 149, 269-273.

[23]Beeston A L, Surette M G. pfs-dependent regulation of autoinducer 2 production in Salmonella enterica Serovar Typhimurium. Journal of Bacteriology, 2002, 184(13):3450-3456.

[24]Han X G, Lu C P. Detection of autoinducer-2 and analysis of profile of luxS and pfs transcription in Streptococcus suis serotype 2. Current Microbiology, 2009, 58: 146-152.

[25]Nikhat Parveen, Kenneth A. Methylthioadenosine/S- adenosylhomocysteine nucleosidase, a critical enzyme for bacterial metabolism. Molecular Microbiology, 2011, 1: 7-20.

[26]Manjunath H, Englert D L, Shanna S, Cohn W B, Vogt C, Wood T K, Manson M D, Jayaraman A. Chemotaxis to the quorum-sensing signal AI-2 Requires the Tsr chemoreceptor and the periplasmic LsrB AI-2-Binding protein. Journal of Bacteriology, 2011, 1893: 768-773.

[27]Winzer K, Hardie K R, Burgess N , Doherty N, Kirke D, Holden M T G, Linforth R. LuxS: its role in central metabolism and the in vitro synthesis of 4-hydroxy-5-methyl-3(2H)-furanone. Microbiology, 2002, 148: 909-922.

[28]Hardie K R, Cooksley C, Green A D, Winzer K. Autoinducer 2 activity in Escherichia coli culture supernatants can be actively reduced despite maintenance of an active synthase LuxS. Microbiology, 2003, 149: 715-728.

影响禽致病性大肠杆菌信号分子AI-2产生的因素分析

Analysis of Factors Affecting the Activity of AI-2 of Avian Pathogenic Escherichia coli

PDF

赞

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

Metrics

本文评价

推荐阅读 0

[1]	翟晓虎,李翎旭,陈小竹,蒋怀德,贺卫华,姚大伟. 肉中猪源性成分Real-time PCR定量检测技术[J]. 中国农业科学, 2023, 56(1): 156-164.
[2]	白辉, 宋振君, 王永芳, 全建章, 马继芳, 刘磊, 李志勇, 董志平. 谷子抗锈病反应相关MYB转录因子的鉴定与表达[J]. 中国农业科学, 2019, 52(22): 4016-4026.
[3]	张双纳，李正男，范旭东，张尊平，任芳，胡国君，董雅凤. 苹果褪绿叶斑病毒RT-LAMP检测方法的建立[J]. 中国农业科学, 2018, 51(9): 1706-1716.
[4]	孙炳学,石延霞,朱发娣,谢学文,柴阿丽,李宝聚. 多主棒孢SdhB-H278R突变位点AS-real-time PCR 定量检测体系的建立[J]. 中国农业科学, 2018, 51(24): 4647-4658.
[5]	王慧飞，孙艳香，冯雪，张一名，杨江涛，马梅芳，陈光. 棉花精氨琥珀酸合成酶基因GhASS1的克隆及表达分析[J]. 中国农业科学, 2016, 49(7): 1242-1253.
[6]	曹新文，王秀珍，李永梅，赵李婧，马海霞，闫洁. 橡胶草法尼基焦磷酸合酶基因的克隆与功能分析[J]. 中国农业科学, 2016, 49(6): 1034-1046.
[7]	吕娟娟, 王进军, 高新菊, 沈慧敏. 二斑叶螨乙酰辅酶A羧化酶基因全长cDNA克隆及其生物信息学分析[J]. 中国农业科学, 2013, 46(8): 1736-1744.
[8]	白灏, 韩先干, 刘蕾, 单雪芹, 宋军, 刘瑞, 董洪亮, 刘海文, 丁铲, 于圣青. 信号分子AI-2对禽致病性大肠杆菌的调控作用[J]. 中国农业科学, 2012, 45(24): 5110-5116.
[9]	袁红朝, 秦红灵, 刘守龙, 童成立, 魏文学, 吴金水. 长期施肥对红壤性水稻土细菌群落结构和数量的影响[J]. 中国农业科学, 2011, 44(22): 4610-4617.
[10]	杨金丽,侯先志,高爱武. 蒙古绵羊瘤胃固、液相附着微生物的RT-PCR检测[J]. 中国农业科学, 2009, 42(6): 2126-2132 .