中国农业科学 ›› 2011, Vol. 44 ›› Issue (12): 2422-2430 .doi: 10.3864/j.issn.0578-1752.2011.12.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

长果种黄麻SRAP标记遗传连锁图谱的构建 及3个质量性状基因定位

陈 晖;陈美霞;陶爱芬;张广庆;徐建堂;祁建民;方平平
  

  1. 福建农林大学作物遗传育种综合利用教育部重点实验室
  • 收稿日期:2010-11-03 修回日期:2010-11-26 出版日期:2011-06-15 发布日期:2011-06-15
  • 通讯作者: 祁建民

Construction of a Molecular Linkage Map Using SRAP and Mapping of Three Qualitative Traits in Corchorus olitorius L.

CHEN Hui; CHEN Mei-xia; TAO Ai-fen; ZHANG Guang-qing; XU Jian-tang; QI Jian-min; FANG Ping-ping
  

  1. 福建农林大学作物遗传育种综合利用教育部重点实验室
  • Received:2010-11-03 Revised:2010-11-26 Online:2011-06-15 Published:2011-06-15

摘要:

【目的】构建黄麻遗传连锁图谱,定位质量性状基因,为今后有关黄麻基因组结构、重要农艺性状QTL定位、分子标记辅助育种和基因克隆等研究工作奠定基础。【方法】以甜麻(黄麻野生种)和宽叶长果(黄麻栽培品种)为杂交亲本,构建了187个F2单株作为作图群体,利用513对SRAP引物进行遗传图谱构建,并对3个质量性状基因(托叶色、叶柄色、叶缘色)进行了定位。【结果】122个SRAP多态性标记位点和这3个形态学标记被定位在该图谱上,初步构建的长果种黄麻遗传连锁图谱全长2 231.9 cM,包含10个连锁群,每个连锁群有2—38个标记位点,2个标记间平均间距为17.86 cM。【结论】该图谱上的标记位点均匀分布在10个连锁群上,没有出现标记位点聚集的现象,表明SRAP标记十分适合黄麻遗传图谱的构建。

Abstract:

【Objective】Jute (Corchorus olitorius L.) is one of the most economically important crops for fiber production. The present study was aimed to construct a molecular linkage map of jute and locate important qualitative traits, which will provide essential tools for investigating genomic structure and evolution, facilitating QTL mapping, marker-assisted selection and map-based cloning of important gene. 【Method】In this study, a population of F2 generation including 187 individual plants was derived from the cross between Corchorus aestuans L. (wild species) and wild leaf jute (cultivated species). A total of 513 sequence-related amplified polymorphisms (SRAP) primer pairs were used in constructing the molecular genetic linkage map and mapping 3 qualitative traits including color of stipule, color of stipe, and color of leaf margin.【Result】This genetic linkage map containing 10 linkage groups (LGs) was constructed using 122 SRAP loci and these 3 morphological markers, the map covered 2 231.9 cM with the average marker interval of 17.86 cM, every linkage group included 2-38 markers.【Conclusion】All the markers are distributed evenly among the linkage groups without clustering of loci. SRAP markers may be a better type of markers in map construction.