可感染茶尺蠖的重组家蚕核型多角体病毒的构建

doi:10.3864/j.issn.0578-1752.2012.16.008

摘要/Abstract

摘要： 【目的】构建能够感染家蚕和茶尺蠖的重组家蚕核型多角体病毒（rBmNPV），解决EoNPV扩增困难而不易获得的难题。【方法】通过Bac-to-Bac系统构建具备BmNPV的多角体蛋白基因（polh）表达盒和EoNPV的解旋酶基因（hel）表达盒的重组家蚕核型多角体病毒。【结果】家蚕或家蚕细胞扩增的rBmNPV可以感染茶尺蠖并致病，表明rBmNPV的宿主域拓展至茶尺蠖。【结论】成功构建了能够感染茶尺蠖的rBmNPV，为利用家蚕作为宿主来生产重组病毒杀虫剂打下基础。

关键词: EoNPV, BmNPV, 重组病毒, 宿主域

Abstract: 【Objective】The objective of this study is to construct recombinant BmNPV infecting both silkworm and Ecotropis oblique, and to solve the reproduction difficulty of EoNPV because of the breeding complexity.【Method】Recombinant BmNPV containing the polh expression box of BmNPV and the hel expression box of EoNPV were constructed by Bac-to-Bac system. 【Result】rBmNPV amplified from silkworm or BmN cell could infect E. oblique, which indicated the host domain of rBmNPV had been broaden to E. oblique. 【Conclusion】rBmNPV which could infect E. oblique was successfully constructed, thus laied a foundation of producing recombinant virus pesticide using silkworm as host.

Key words: EoNPV, BmNPV, recombinant virus, host domain

郭睿, 刘卫星, 潘中华, 薛仁宇, 曹广力, 朱越雄, 贡成良. 可感染茶尺蠖的重组家蚕核型多角体病毒的构建[J]. 中国农业科学, 2012, 45(16): 3288-3296.

GUO Rui, LIU Wei-Xing, PAN Zhong-Hua, XUE Ren-Yu, CAO Guang-Li, ZHU Yue-Xiong, GONG Cheng-Liang. Construction of Recombinant BmNPV Infecting Ecotropis oblique[J]. Scientia Agricultura Sinica, 2012, 45(16): 3288-3296.

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