关键词: 杂交玉米, 农大108, 种子内生细菌, 群落多样性, 非培养方法

Abstract: 【Objective】 The objective of this study is to investigate the influence of genotypes of maize on seeds endophytic bacterial communities. 【Method】 Using the 16S rDNA clone library technique, this paper conducted a priliminary study on the community diversity of endophytic bacteria in seeds of reciprocal cross hybrid maize (Zea mays L., Nongda108) and their parental lines. 【Result】 The endophytic bacterial communities in 108A and 108B included 46 and 42 OTUs respectively, and the abundance of the first dominant bacterium Pseudomonas in 108A and 108B was 34.29% and 34.00%. The parental lines Huang C and 178 included 17 and 11 OTUs respectively, the abundance of Enterobacter and Burkholderia as the first dominant bacterium in which respectively was 49.13% and 89.72%. Roseateles, Enterobacter, Pseudomonas and Sphingomonas found in the seeds of 108A were also existed in its female parent Huang C. Pseudomonas, Ralstonia, Paenibacillus and Bacillus were also existed in its male parent 178. Acinetobacter, Pseudomonas, Ralstonia, Paenibacillus and Bacillus found in the seeds of 108B were also existed in its female parent 178. Roseateles, Citrobacter, Pantoea, Pseudomonas, Stenotrophomonas and Escherichia were also existed in its male parent Huang C. 【Conclusion】 In the seeds of reciprocal cross hybrid maize, Nongda 108 and their parental lines which are genetically related, the endophytic bacterial community structure has certain relevance.

Key words: hybrid maize, Nongda 108, seed endophytic bacteria, bacterial diversity, culture-independent method