Abstract:

【Objective】The aim of this experiment is to investigate the genes related to meat quality, and study its genetic basis and molecular mechanism.【Method】A subtracted cDNA library was constructed by using suppression subtractive hybridization, differential expressions of the genes were studied by using real-time quantitative PCR. 【Result】A total of 61 positive clones were screened. A total of 47 effective sequences were found by sequencing in the whole subtractive cDNA library. After comparisons with GenBank, GenBank ESTs librarys using the online software of the BLAST, the two unknown sequences were found. The expressions of the MYL1, LDHA, KPNA3, TPM3, HUMMLC2B, GNTN, and Oaz genes were studied by real-time quantitative PCR. The results showed that significant differences were found between the Lantang Pig and Large White Pig. The expressions of the MYL1, LDHA, KPNA3, TPM3, and HUMMLC2B genes were significantly higher in the Lantang Pig (P＜0.05), and the expressions of the GNTN and Oaz genes were significantly higher in the Large White Pig (P＜0.05). 【Conclusion】Many of these ESTs were highly homologous with the important genes related to meat quality traits, and significant differences of them were found between Lantang Pig and Large White Pig.