不同黑大豆种质资源种皮花色苷组成及抗氧化活性分析

doi:10.3864/j.issn.0578-1752.2010.24.013

中国农业科学 ›› 2010, Vol. 43 ›› Issue (24): 5088-5099 .doi: 10.3864/j.issn.0578-1752.2010.24.013

不同黑大豆种质资源种皮花色苷组成及抗氧化活性分析

张芳轩,张名位,张瑞芬,刘章雄,魏振承,邱丽娟,杨春英,张雁,唐小俊,邓媛元

(广东省农业科学院农业生物技术研究所/农业部功能食品重点开放实验室)

收稿日期:2010-04-19 修回日期:2010-09-29 出版日期:2010-12-15 发布日期:2010-12-15
通讯作者: 张瑞芬

Comparative Analysis of the Anthocyanin Profiles and Antioxidant Activity of Seed Coats from Different Black Soybean Germplasm Resources

ZHANG Fang-xuan, ZHANG Ming-wei, ZHANG Rui-fen, LIU Zhang-xiong, WEI Zhen-cheng, QIU Li-juan, YANG Chun-ying, ZHANG Yan, TANG Xiao-jun, DENG Yuan-yuan

(广东省农业科学院农业生物技术研究所/农业部功能食品重点开放实验室)

Received:2010-04-19 Revised:2010-09-29 Online:2010-12-15 Published:2010-12-15
Contact: ZHANG Rui-fen

摘要/Abstract

摘要：

【目的】了解不同黑大豆种质资源种皮中花色苷的组成、含量及其抗氧化能力分布情况。【方法】采用酸性甲醇浸提60个黑大豆种质种皮中的花色苷,通过与标准品对照,用HPLC法分析其种皮花色苷的组成及含量,同时用pH示差法测定各种质的总花色苷含量,采用氧自由基清除能力(ORAC)法测定各种质种皮的抗氧化活性。【结果】从60个黑大豆种质中共检测到飞燕草素-3-葡萄糖苷、矢车菊素-3-半乳糖苷、矢车菊素-3-葡萄糖苷、矮牵牛素-3-葡萄糖苷、芍药素-3-葡萄糖苷和锦葵素-3-葡萄糖苷６种花色苷组分。其中有44个品种中检测到上述全部6种花色苷组分,而其余16个品种只含有其中的4－5种。矢车菊素-3-葡萄糖苷是所有受试黑大豆种质中含量最高的花色苷组分。不同黑大豆种质间各单体花色苷和总花色苷含量及其ORAC值抗氧化能力差异均较大,其中总花色苷含量的变幅为98.8—2 132.5 mg/100g,ORAC值的变幅为212.5—1 834.6 μmol TE/g,且各种质总花色苷含量与其ORAC值呈显著正相关关系(r = 0.62, P＜0.001)。采用快速聚类法将60个黑大豆种质聚成营养和花色苷活性成分含量差异显著的三大类群。【结论】不同黑大豆种质种皮花色苷组成基本相同,但其各花色苷单体及总花色苷含量存在显著差异,花色苷是黑大豆种皮抗氧化作用的重要物质基础。

关键词: 黑大豆, 种皮花色苷, 高效液相色谱, ORAC

Abstract:

【Objective】 The anthocyanins profiles and antioxidant activity of seed coats from different black soybean varieties were investigated. 【Method】 Crude extract solution of black soybean seed coats anthocyanins of 60 varieties was obtained by soaking seed coats in acidified methanol. External standards were used to determine the composition and content of anthocyanins in each variety by HPLC gradient elution method. The oxygen radical scavenging capacities expressed as ORAC values of black soybean varieties were also evaluated. 【Result】 Six anthocyanins were found totally in 60 black soybean varieties including delphinidin-3-glucoside, cyanidin-3-galactoside, cyanidin-3-glucoside, petunidin-3-glucoside, peonidin-3-glucoside and malvidin-3- glucoside. All the 6 components were detected in 44 varieties of the black soybean, and only 4 or 5 anthocyanins were detectable in the other 16 varieties. Cyanidin-3-glucoside was the predominant anthocyanin in all the analyzed varieties. Total contents of anthocyanins and each component showed significant differences between varieties. Total anthocyanins content and ORAC in various black soybean varieties were highly different with the ranges of 98.8-2 132.5 mg/100g and 212.5-1 834.6 μmol TE/g, respectively. A significant correlation was found between anthocyanin concentration and ORAC values (r=0.62, P＜0.001). Sixty black soybean varieties were divided into three groups by K-means clustering analysis, which showed divers levels of nutritional or bioactive traits.【Conclusion】The components of anthocyanins in different black soybean varieties were similar, but things were different when it came to the content of individual anthocyanin. Anthocyanins are most significant antioxidant components in black soybean seed coats.

Key words: black soybean, seed coat anthocyanins, HPLC, ORAC

张芳轩,张名位,张瑞芬,刘章雄,魏振承,邱丽娟,杨春英,张雁,唐小俊,邓媛元
. 不同黑大豆种质资源种皮花色苷组成及抗氧化活性分析[J]. 中国农业科学, 2010, 43(24): 5088-5099 .

ZHANG Fang-xuan,ZHANG Ming-wei,ZHANG Rui-fen,LIU Zhang-xiong,WEI Zhen-cheng, QIU Li-juan,YANG Chun-ying,ZHANG Yan,TANG Xiao-jun,DENG Yuan-yuan
. Comparative Analysis of the Anthocyanin Profiles and Antioxidant Activity of Seed Coats from Different Black Soybean Germplasm Resources
[J]. Scientia Agricultura Sinica, 2010, 43(24): 5088-5099 .

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不同黑大豆种质资源种皮花色苷组成及抗氧化活性分析

Comparative Analysis of the Anthocyanin Profiles and Antioxidant Activity of Seed Coats from Different Black Soybean Germplasm Resources

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