关键词: THRSP基因, PCR-SSCP, 秦川牛, 分子标记

Abstract:

【Objective】 The study aimed at analysis of a C/T missense mutation founded by direct sequencing at THRSP gene exon1 184 bp locus and investigations of the correlation of the SNP with some meat traits in Qinchuan cattle. 【Method】 Four hundred and five Qinchuan cattles without genetic relationship at age of 18-20 months were selected in this study, and the cattle were kept under the same condition. Individual genotypes of THRSP gene were analyzed by PCR-SSCP method.General linear model (GLM) of SPSS statistical software was used to study the correlation between this SNP and partial meat traits of 93 Qinchuan cattle. 【Result】 This mutation leads to a missense mutation from Val (GTG) to Ala (GCG) at the 51th amino acid. In this locus, PIC (polymorphism information content) is 0.3583, belongs to moderate polymorphisms, He (heterozygosity) is 0.4676, Ne (number of effective allele) is 1.8783. The chi-square test showed that this locus is not in Hardy-Weinberg equilibrium (P＜0.01). Correlation analysis revealed that genotype in this locus has a significant correlation with tenderness and WHC (water holding capacity) traits. Multi-comparison indicated that the tenderness of AA genotype individual is significantly higher than that of AB (P＜0.05), and the WHC of BB is significantly higher than AB (P＜0.05). 【Conclusion】 This locus is a potential major QTN that affects tenderness and WHC or tightly linked to it, it could be a candidate molecular marker for the beef cattle breeding.

Key words: THRSP gene, PCR-SSCP, Qinchuan cattle, molecular marker